• Efficiently extract proteins from all cellular compartments with superior protein recovery
• Create lysates for downstream applications: immunoprecipitation, SDS-PAGE, Western blot
• Make cell extracts that are compatible with all Signal Seeker IP kits (required genomic DNA removal using BlastR® filters)

Kit contents (sufficient for 100 x 500 µl lysates)

• BlastR® lysis buffer
• BlastR® dilution buffer

Equipment & materials required

• Cell culture or tissue samples

The BlastR® lysis buffer system produces efficient extraction of proteins from all cellular compartments in eukaryotic cells. The buffer is compatible with standard colorimetric protein assays. This system performs optimally when used in conjunction with the BlastR® filter system.

Key characteristics

• Highly efficient total protein extraction from cell and tissue samples
• Compatible with many downstream applications: IP, SDS-PAGE, Western blot (once lysate viscosity is reduced)
• Superior protein extraction profile to popular lysis buffers, including RIPA