• Fluorogenic, far-red stain for actin filaments in live cells
• Fluorogenic, far-red stain for actin filaments in paraformaldehyde fixed cells
• Compatible with multicolor imaging

SiR700-actin is based on the silicon rhodamine (SiR) fluorophore analogue SiR700 and the actin binding natural product jasplakinolide. SiR700-actin allows the labelling of F-actin in live cells with high specificity and low background

Key features

• Ab: 689 nm, Em: 716 nm – far-red spectrum
• Emission in far-red minimizes phototoxicity and sample autofluorescence
• Cell permeable
• Works on live or fixed cells
• Fluorogenic character: enhanced fluorescence when bound to F-actin
• Compatible with widefield, confocal, STED and SIM imaging

The biological activity of CY-SC013 is assessed by the ability of the probe to efficiently label actin filaments in live cell culture. After a wash step, cell staining is visible for several hours.