SiR700-DNA is based on the far-red silicon rhodamine (SiR) fluorophore analogue SiR700 and the DNA minor groove binder bisbenzimide (Hoechst). SiR700-DNA allows the labelling of DNA in live cells with high specificity and low background without the need for genetic manipulation or over-expression. The key features of SiR700-DNA are i) far-red absorption and emission wavelengths, ii) cell permeability, iii) fluorogenic character, and iv) compatibility with super-resolution microscopy (e.g., STED & SIM). In addition, SiR700-DNA can be used for wide-field and confocal fluorescent imaging in living cells. The emission in the far-red wavelength minimizes phototoxicity and sample autofluorescence. SiR700-DNA is compatible with GFP and/or m-cherry fluorescent proteins. It can be imaged with standard Cy5 filter sets. This probe has multiple advantages (far-red, fluorogenic, cell permeable, low background, and high specificity for DNA) over existing probes such Syto61, DRAQ5, and Vybrant DyeCycle Ruby dyes. Probe quantity allows 35 – 140 staining experiments.*
For comparison information with other nuclear stains click here.
λabs 689 nm
λex 716 nm
εmax 1.0·105 mol-1·cm-1
MW 974.2 g/mol
*Based on the following conditions: 0.5 – 1 ml staining solution / staining experiments with 0.5 – 1 µM probe concentration. The number of staining experiments can be further increased by reducing volume or probe concentration.
Cytoskeleton, Inc. is the exclusive provider of Spirochrome, Ltd. products in North America.
Swiss 3T3 fibroblast stained by SiR-700 DNA (Cat. # CY-SC015) using widefield microscopy.
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