• Fluorogenic, far-red stain for DNA in live cells
• Fluorogenic, far-red DNA staining in fixed cells
• Compatible with multicolor imaging

SiR700-DNA is based on the silicon rhodamine (SiR) fluorophore analogue SiR700 and the DNA minor groove binder bisbenzimide (Hoechst). SiR700-DNA allows the labelling of DNA in live cells with high specificity and low background.

Key features

• Ab: 689 nm, Em: 716 nm – far-red spectrum
• Emission in far-red minimizes phototoxicity and sample autofluorescence
• Cell permeable
• Non-toxic
• Works on live or fixed cells
• Fluorogenic character: enhanced fluorescence when bound to DNA
• Compatible with widefield, confocal, STED and SIM imaging

The biological activity of CY-SC015 is assessed by the ability of the probe to efficiently label DNA (nucleus) in live cell culture. After an optional wash step, cell staining is visible for several hours. Omit the wash step for time lapse imaging.