Enzymatically oxidize actin at methionine 44 & 47 (-actin nomenclature)
Study the role of M44/47 oxidation on actin function

Enzymatic redox control of actin at Met44/Met47 has been shown to destabilize F-actin in vivo and to play a key role in a growing number of cellular processes, including cytokinesis, axonal guidance, dendritic organization, synaptic development, heart and muscle development, and cell viability. Redox regulation at actin Met44/47 is mediated by the monooxygenase MICAL1 and the methionine sulfoxide reductase MsrB (SelR).

Human MSRB2 protein has been produced and purified from a bacterial expression system.

• Protein tag: N-terminal His tag for purification
• UniProt ID: Q9Y3D2
• Amino acids: aa 42-182
• Molecular weight: ~17 kDa
• Formulation: supplied as a lyophilized powder
• Composition: when resuspended to 1 mg/ml, the buffer composition is: 10 mM Tris pH 8.0, 50 mM NaCl, 2.5% sucrose, and 0.5% dextran

Protein purity is assessed by scanning densitometry of Coomassie Blue-stained protein on a 4-20% polyacrylamide gel. Purity is determined to be ≥90% pure.

The biological activity of MB201 is determined by its ability to reduce oxidized skeletal muscle actin MXA95 as determined by a subtilisin limited proteolysis assay (see datasheet). In this assay, MB201 modifies ≥90% of the actin.