• Biochemical analysis of alpha-actinin function
• Study the regulation of actin dynamics
• Positive control for actin binding/bundling assays

Alpha-actinin is a cytoskeletal actin-binding protein that cross-links actin filaments and anchors them to various cellular structures, contributing to cell shape, motility, and adhesion. It plays critical roles in muscle sarcomere organization and non-muscle cellular architecture, making it essential for structural integrity and signaling functions.

Alpha-actinin is purified from rabbit skeletal muscle

• Molecular weight: ~100 kDa
• Composition: when resuspended to 1 mg/ml, the buffer composition is 4 mM Tris-HCl, pH 7.6, 4 mM NaCl, 20 µM EDTA, 1% (w/v) sucrose, and 0.2% (w/v) dextran
• Supplied as a white lyophilized powder

Protein purity is determined by scanning densitometry of Coomassie Blue-stained protein on a 4-20% polyacrylamide gel. Purity is >85%

The biological activity of alpha-actinin is assessed by its ability to bind to F-actin filaments. An F-actin binding assay is performed. Under the assay conditions described below,>65% of alpha-actinin co-pellets with F-actin, while <10% pellets in the absence of F-actin (see datasheet for details)