• Detect endogenous acetylation changes on a protein of interest (POI) in cells or tissue extracts
• Isolate the total acetylation profile from cells or tissues via immunoprecipitation (IP) for downstream proteomic analysis
• Confirm POI acetylation data generated from mass spec proteomics.

Cytoskeleton offers two Acetyl-Lysine Affinity beads: AAC02-beads and AAC03-beads. Both contain unique mouse anti-acetyl-lysine antibodies covalently linked to protein G beads and enrich a broad range of acetylated proteins. Used together, they provide an even more comprehensive enrichment profile.

While their specificities overlap, each has unique strengths—AAC02-beads may outperform AAC03-beads for specific targets. For a new protein of interest, test both separately to determine which gives the best results (see datasheet for examples).

AAC02-beads are an affinity reagent comprising a high affinity/specificity pan-acetyl-lysine antibody AAC02 covalently linked to agarose beads.

Key characteristics

• Broad enrichment of acetylated proteins
• No heavy/light chain leaching during IP
• Complimentary overlap with AAC03-beads AAC03-beads - see datasheet for details
• Sufficient reagent for 40 IP assays (1 mg lysate per IP)
• Consistent lot-to-lot performance
• Supplied as a lyophilized powder
• Buffer composition after rehydration: 200 mM PIPES, 50% glycerol, 5% sucrose, and 1% dextran

The biological activity of AAC02-beads is demonstrated by the reagent’s ability to IP a broad range of acetylated proteins from cell and tissue lysates (50 µl bead slurry to mg input lysate). Specificity is demonstrated by the fact that the IP acetylation profile increases by ≥10 fold in a lysate treated with the deacetylase inhibitors trichostatin A (TSA) and nicotinamide.