• Detect endogenous acetylation changes on a protein of interest (POI) in cells or tissue extracts
• Isolate the total acetylation profile from cells or tissues via immunoprecipitation (IP) for downstream proteomic analysis
• Confirm POI acetylation data generated from mass spec proteomics

Cytoskeleton offers two Acetyl-Lysine Affinity beads: AAC02-beads and AAC03-beads. Both contain mouse anti-acetyl-lysine antibodies covalently linked to protein G beads and enrich a broad range of acetylated proteins. Used together, they provide a more comprehensive enrichment profile.

While their specificities overlap, each has unique strengths—AAC02-beads may outperform AAC03-beads for specific targets. For a new protein of interest, test both separately to determine which gives the best results (see Detailed Methods and Validated Applications for examples).

AAC04-beads is a combo of Cat # AAC02-beads and Cat # AAC03-beads.

Key characteristics

• 2 tubes of each affinity bead AAC02-beads & AAC03-beads
• Broad enrichment of acetylated proteins
• No heavy/light chain leaching during IP
• Complimentary overlap with AAC02-beads AAC02-beads - see datasheet for details
• Sufficient reagent for 40 IP assays (1 mg lysate per IP)
• Consistent lot-to-lot performance
• Supplied as a lyophilized powder
• Buffer composition after rehydration: 200 mM PIPES, 50% glycerol, 5% sucrose, and 1% dextran

AAC04-beads contain 2 separate tubes, each of AAC02/AAC03-beads, allowing the end-user the versatility to examine both affinity beads and a mix of the two to determine which conditions are optimal for their application.