• Detect endogenous phosphotyrosine changes on a protein of interest (POI) in cells or tissue extracts
• Isolate the total phosphotyrosinated protein profile from cells or tissues via immunoprecipitation (IP) for downstream proteomic analysis
• Confirm POI pTyr data generated from mass spec proteomics

Phosphotyrosine (pTyr) post-translational modifications (PTMs) are crucial regulatory switches in cell signaling, controlling processes like growth, differentiation, and immune responses through reversible tyrosine phosphorylation. Dysregulation of these modifications is linked to diseases such as cancer, making them key therapeutic targets.

APY03-beads are an affinity reagent comprising a high-affinity/specificity pan-phosphotyrosine antibody APY03 covalently linked to agarose beads.

Key characteristics

• Broad enrichment of pTyr proteins
• No heavy/light chain leaching during IP
• Sufficient reagent for 40 IP assays (1 mg lysate per IP)
• Consistent lot-to-lot performance
• Supplied as a lyophilized powder
• Buffer composition after rehydration: 200 mM PIPES, 5% sucrose, and 1% dextran

The biological activity of APY03-beads is demonstrated by the ability of the reagent to enrich a broad range of tyrosine phosphorylated proteins in HeLa cells treated with the protein tyrosine phosphatase inhibitor, pervanadate. The pTyr signal is enhanced ≥10-15 fold over untreated lysates (30 µl beads: 1mg lysate).