• Measure in vitro cardiac actin polymerization kinetics via fluorescence increase
• Actin-binding protein (ABP) effect on actin dynamics
• Drug effects on cardiac actin-ABP interactions

Bovine cardiac actin AD99 is labeled with pyrene (N-1-pyrene iodoacetamide) at the reactive cysteine 374 residue.

• Ab: 360 nm, Em: 405 nm
• Labeling stoichiometry: ~0.2-0.4 pyrenes per actin monomer
• Supplied as a lyophilized powder

Protein purity is assessed by scanning densitometry of Coomassie Blue-stained protein on a12% polyacrylamide gel. Purity was determined to be >99% pure.

The biological activity of pyrene-labeled actin has been determined by its ability to polymerize into filaments in vitro efficiently. Cardiac pyrene actin exhibits a 4-6-fold increase in fluorescence (Ex: 360 nm, Em: 405 nm) upon polymerization, equivalent to >90% polymerization under the experimental conditions.