• Detect endogenous SUMO1 changes on a protein of interest (POI) in cells or tissue extracts
• Isolate the total SUMO1 modified protein profile from cells or tissues via immunoprecipitation (IP) for downstream proteomic analysis
• Confirm POI SUMOylation data generated from mass spec proteomics

SUMO1 is a small ubiquitin-like modifier protein involved in post-translational modifications that regulate protein stability, localization, and activity. Its conjugation to target proteins influences key cellular processes, including transcriptional regulation, DNA repair, and stress response pathways.

ASM11-beads are an affinity reagent comprising a high-affinity/specificity pan-SUMO1 antibody ASM01 covalently linked to agarose beads.

Key characteristics

• Broad enrichment of SUMO1 modified proteins
• No heavy/light chain leaching during IP
• Sufficient reagent for 30 IP assays (1 mg lysate per IP)
• Consistent lot-to-lot performance
• Supplied as a lyophilized powder
• Buffer composition after rehydration: 200 mM PIPES, 50% glycerol, 5% sucrose, and 1% dextran

The biological activity of ASM11-beads is demonstrated by the ability of the reagent to enrich a broad range of SUMO1-modified proteins in HAP1 wild type (WT) cells, with SUMO1 signal absent in HAP1 SUMO1 knockout (KO) cells (40 µl beads: 1mg lysate).