• Monitor tubulin polymerization in vitro via kinetic optical density increase
• Dependable assay for identification of anti-cancer compounds and MAPs targeting microtubule dynamics
• Identification of microtubule-binding proteins (MAPs)
• Identification of anti-tubulin ligands

Kit content (sufficient for 24 assays)

• Tubulin protein (97% pure)
• Tubulin glycerol buffer
• Guanosine triphosphate (GTP)
• Paclitaxel (polymerization enhancer)
• General tubulin buffer
• DMSO
• Half area 96 well plate
• Detailed protocol

Equipment & materials required

• Protein or compound of interest
• Temperature controlled spectrophotometer capable of reading a 96 well plate at 340 nm in kinetic mode

This kinetic assay measures tubulin polymerization dynamics by measuring the increase in light scattering (absorbance) as tubulin polymerizes into microtubules. It is designed to monitor the real-time kinetics of microtubule assembly and disassembly, supporting studies of cellular processes and drug screening.

Key features

• Absorbance-based readout at 340 nm
• 400 µg tubulin per assay/5% glycerol, 24 assays
• 100 µl volume per assay
• Coefficient of variation (cv): 14%