BlastR™ Lysis/Dilution Buffer Kit

BlastR™ Lysis/Dilution Buffer Kit

$0.00

The BlastR™ lysis buffer is a a denaturing buffer that isolates protein from all cellular compartments and produces lysates compatible with western blotting applications.  When used in combination with BlastR dilution buffer it produces a cell lysate ideal for immunoprecipitation applications. 

This lysis system was developed to be used with BlastR filters, which  allows for fast and reliable preparation of genomic DNA (gDNA)-free cell lysate for western blot or immunoprecipitation. Unlike sonication or insulin needle gDNA shearing methods, the BlastR™ filter effectively removes gDNA contamination while having no effect on the integrity of the proteins in the lysate. 

Product Uses Include

  • Rapid isolation of protein extracts
  • Preparation of extracts from denaturing lysis buffers 

Validation Data: BlastR Rapid Filtration Kit White Paper

    Example results

    Comparison of BlastR™ versus commonly used non-denaturing and denaturing lysis buffers. Isolation of proteins from the membrane, cytoplasm, mitochondria, and nucleus.

    A431 cells were lysed with BlastR™, RIPA, mPER, IP lysis, Denaturing (1% SDS), and Laemmli lysis buffers. Isolation of proteins from the membrane, cytoplasmic, mitochondrial, and nuclear markers were determined using the respective compartment marker proteins. The western blot figure highlights the ability of BlastR™ buffer to isolate proteins from all cellular compartments similarly to other denaturing buffers. Unlike ofther denaturing buffers, the BlastR™ buffer is compatible with standard protein assays, which is critical when equivalent protein loading is essential.  BlastR™ buffer is also an excellent IP buffer when used with the BlastR™ dilution buffer as outlined in the datasheet. These features are outlined in the table below for easy comparison with available lysis buffers.

    table_for_blastR_buffer_1
    blastr_buffer_cell_compartments

     

    For more information contact:  signalseeker@cytoskeleton.com

    Associated Products:

    Signal-Seeker™ Phosphotyrosine Detection Kit (Cat. # BK160)

    Signal-Seeker™ Ubiquitination Detection Kit (Cat. # BK161)

    Signal-Seeker™ SUMOylation 2/3 Detection Kit (Cat. # BK162)

    Click on the pdf icon below to download the manual

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    If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com