Phosphotyrosine tools
Cyto’s phosphotyrosine (pTyr) tools are engineered for exceptional accuracy and sensitivity, empowering you to detect and analyze even the rarest pTyr modifications with confidence.
Compared to phospho-serine/threonine, pTyr is a low-abundance PTM, hence standard enrichment methods like IMAC often fail to retain pTyr modifications—leading to data loss.
Cyto’s pTyr tools help you overcome these limitations and ensure your research captures the full picture.
- Superior affinity beads: Signal-Seeker® affinity beads efficiently enrich low-abundance phosphotyrosine-modified proteins (<1% of total target proteins) using covalently bound anti-phosphotyrosine antibody (Clone 27B10) on Protein G agarose. Validated for broad target capture, they ensure high specificity and performance. Notably, the beads prevent antibody chain leaching during IP assays, delivering clean, reliable results for phosphotyrosine protein analysis.
- Simplify the complex: Comprehensive Signal-Seeker® kits provide optimized lysis buffers, high-quality affinity reagents, and essential inhibitors to ensure accurate preservation and detection of pTyr-modified proteins. These kits utilize immunoprecipitation (IP) followed by western blot analysis to examine your specific protein of interest (POI). Simply supply your experimental cell line or tissue sample along with a high-quality antibody against your POI—and begin investigating its post-translational modifications (PTMs) with confidence.
- High-quality antibodies: Customers love our Signal-Seeker® phosphotyrosine-antibody, cost-effective and second to none in performance (see their data here).
Cyto’s phosphotyrosine (pTyr) tools are engineered for exceptional accuracy and sensitivity, empowering you to detect and analyze even the rarest pTyr modifications with confidence.
Compared to phospho-serine/threonine, pTyr is a low-abundance PTM, hence standard enrichment methods like IMAC often fail to retain pTyr modifications—leading to data loss.
Cyto’s pTyr tools help you overcome these limitations and ensure your research captures the full picture.
- Superior affinity beads: Signal-Seeker® affinity beads efficiently enrich low-abundance phosphotyrosine-modified proteins (<1% of total target proteins) using covalently bound anti-phosphotyrosine antibody (Clone 27B10) on Protein G agarose. Validated for broad target capture, they ensure high specificity and performance. Notably, the beads prevent antibody chain leaching during IP assays, delivering clean, reliable results for phosphotyrosine protein analysis.
- Simplify the complex: Comprehensive Signal-Seeker® kits provide optimized lysis buffers, high-quality affinity reagents, and essential inhibitors to ensure accurate preservation and detection of pTyr-modified proteins. These kits utilize immunoprecipitation (IP) followed by western blot analysis to examine your specific protein of interest (POI). Simply supply your experimental cell line or tissue sample along with a high-quality antibody against your POI—and begin investigating its post-translational modifications (PTMs) with confidence.
- High-quality antibodies: Customers love our Signal-Seeker® phosphotyrosine-antibody, cost-effective and second to none in performance (see their data here).
