As part of the Signal-Seeker™ product line, SUMO1 IP control beads (CIG03) have been developed as highly specific negative control beads for Cytoskeleton's SUMOylation 1 antibody-based affinity bead products. These control beads are far superior to beads alone, because it is a better representative of the actual IP condition. The IgG antibodies are covalently attached to minimize antibody heavy and light chain leaching, which makes interpretation of results easier. Additionally, these control beads are effective for pre-clearing lysates without risk of contamination with IgG antibody. IgG control beads (CIG03) are included in our comprehensive Signal-Seeker™ SUMOylation 1 Detection Kits and utilizing these kits are recommend for first time SUMO1 detectiion.
Application 1: Utilization of acetyl-lysine control beads
SUMO1 IP control beads are compatible with SUMOylation 1 Affinity Beads and Signal-Seeker SUMOylation 1 Detection Kits. Note these beads do not have cross reactivity with SUMO1 (Figure 1).
Figure 1: HAP1 wildtype (WT) or SUMO1 knockout (KO) lysate, was obtained using BlastR lysis and filter system. 1 mg of each lysate were incubated with 40 µg of SUMO1 affinity beads (ASM11-beads (Cytoskeleton)), and conjugated SUMO1 IP control beads (CIG03). Samples were separated by SDS-PAGE and transferred to PVDF. Enriched SUMO1 samples were analyzed by western blot using SUMO1 (ASM01 (Cytoskeleton)) antibody at 1:5000. Mouse-HRP secondary at 1:20,000 in 5% milk was used.
Each package contains enough IgG control beads for 12 reactions.
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Signal-Seeker™ SUMOylation 1 Detection Kit (Cat. # BK165)
Signal-Seeker™ SUMO1 Affinity Beads (Cat.# ASM11-beads)
Signal-Seeker™: BlastR™ Rapid Lysate Prep Kit (Cat. # BLR01)