Conjugation of SiR-COOH dye to proteins or peptides
Conjugation of SiR-COOH dye to oligonucleotides or small molecules
Material
SiR-COOH is the 6-carboxylic acid form of SiR dye (Ab: 652, Em: 674). The carboxylic acid group facilitates EDC/NHS activation or other amide bond forming chemistries for labeling proteins, peptides or amine-bearing molecules
Key features
Ab: 652 nm, Em: 674 nm – far-red spectrum
Emission in far-red minimizes phototoxicity and sample autofluorescence
High extinction coefficient
High photostability
Compatible with widefield, confocal, STED and SIM microscopy
