• In vitro actin-binding protein (ABP) identification
• Biochemical characterization of APBs
• Detect ABPs from cell or tissue extracts or purified proteins

Kit contents (30 – 100 assays)

• Non-muscle (β and γ) actin substrate
• Alpha actinin-positive control ABP
• Bovine serum albumin (BSA)-negative control
• Comprehensive assay buffers (8 buffers)
• Detailed protocol

Equipment & materials required

• Purified protein or lysate of interest
• Ultracentrifuge capable of processing 50-100 µl sample volumes at 150,000 x g
• SDS-PAGE equipment
• Protein detection system e.g. Coomassie or western blot reagents

The actin-binding protein (ABP) spin-down assay kit is a convenient and reliable tool for determining whether your protein of interest (POI) binds to filamentous actin (F-actin) or monomeric actin (G-actin). This assay uses a simple centrifugation-based method to separate F-actin and G-actin, allowing you to detect interactions with your POI from either purified protein samples or cell lysates.

Key characteristics

• Detection method: SDS-PAGE or Western blot
• Actin substrate: non-muscle actin
• Timeframe: Approximately 5 – 10 hours, depending on detection method, typically performed over two days