F-actin Visualization Biochem Kit (fluorescence format)

• Visualization of actin cytoskeleton organization and cell morphology
• Co-staining with nuclear dyes for cell structure analysis
• Assessment of actin morphology changes in response to treatments

Kit contents (300 assays)

• Rhodamine phalloidin
• Wash buffer
• Permeabilization buffer
• Fixative solution
• Mounting medium
• Dark box

Equipment & materials required

• Coverslips
• Cell culture
• Fluorescence microscope and image recording equipment: ex:535 nm, Em: 585 nm

This fluorescent phalloidin-based actin staining kit enables precise visualization of F-actin in fixed cells. It includes all necessary fixation buffers for consistent, high-specificity results.

Key characteristics

• Bright, specific labeling: Phalloidin probe (Ex: 535 nm, Em: 585 nm) selectively binds polymerized actin, minimizing background noise
• Cross-isotype and species compatibility: Binding unaffected by actin isoforms or species differences
• Streamlined workflow: Standardized staining protocol reduces variability across experiments
• Long-lasting signal: Anti-fade mounting medium preserves fluorescence for imaging