• Use this probe to detect changes in plasma membrane polarity.
• Optimized for super-resolution microscopy applications

MemGlow™NR4A is a solvatchromic probe that changes its emission spectra in response to a change in membrane (solvent) polarity. The probe acts like an on/off switch that has been optimized for Super Resolution Point Accumulation for Imaging in Nanoscale Topography (SR-PAINT). Upon binding with a plasma membrane in a predominant liquid ordered (LO) phase, NR4A exhibits a 45-50 nm wavelength shift relative to the liquid disordered (Ld) phase, enabling investigators to examine the nanoscale distribution of local chemical polarity in plasma membranes.

Key features

• Ab: 554 nm, Em: 638 nm – orange/red – spectrum
• Polarity shift: <585 nm (ordered), >585 nm (disordered)
• Engineered for super-resolution microscopy and is not optimized for conventional microscopy; however, it can be used with good results

The biological activity of MG06 is assessed by the ability of the probe to efficiently label plasma membranes in live HEK293 cell culture.