• Study in vitro regulation of Cdc42 cell signaling mechanisms
• Serves as a substrate for Cdc42 GEF assays and drug screens
• Serves as a substrate for Cdc42 GAP assays and drug screens

Cdc42 is a small GTP-binding protein in the Rho family that regulates cell polarity, cytoskeletal organization, and signal transduction. It is best known for controlling the formation of filopodia—thin, actin-rich projections that help cells sense their environment and guide directional movement.

Wild-type human Cdc42 protein is produced in a bacterial expression system.

• Protein tag: N-terminal GST tag for purification
• Molecular weight: approximate mwt 45 kDa
• Formulation: supplied as a lyophilized powder
• Composition: when resuspended to 1 mg/ml, the buffer composition is: 10 mM Tris pH 7.5, 20 mM NaCl, 0.3 mM MgCl2, 1.0% sucrose, and 0.2% dextran

Protein purity is assessed by scanning densitometry of Coomassie Blue-stained protein on a 4-20% polyacrylamide gel. Purity was determined to be ≥90% pure.

The biological activity of CDG01 is determined by testing its ability to serve as a substrate for the nucleotide exchange factor hDbs GE01. Quality control standards require that, in the standard assay, hDbs catalyzes nucleotide exchange on Cdc42 at a rate greater than 1.5 mmol mant-GTP per mol Cdc42 per second.