Product Uses Include
- Cdc42 biochemistry
- Cdc42 GTPase assays
- Cdc42 nucleotide exchange assays
- Cdc42 binding studies
Material
The human Cdc42 protein has been produced in a bacterial expression system. The protein is supplied as a lyophilized powder. When it is reconstituted in distilled water to 1 mg/ml, the protein is in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl2, 0.5% sucrose, 0.1% dextran. Protein concentration is determined by the Precision Red Advanced Protein Assay Reagent, Cat. # ADV02.
The recombinant protein is 50 kDa, consisting of the 22 kDa Cdc42 protein plus a 28 kDa GST tag in the amino-terminus.
For other forms of Cdc42 as well as many other purified small G-proteins, see our main small G-protein product page.
Purity
Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. GST-Cdc42 samples are >90% pure.
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Figure 1: GST-Cdc42 protein purity determination. A 20 µg sample of CDG01 (GST-Cdc42 molecular weight approx. 50 kDa) was separated by electrophoresis in a 12% SDS-PAGE system. The protein was stained with Coomassie Blue.
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Biological Activity
Biological activity of the protein is determined by demonstrating that in GTP bound CDG01 associates with PAK protein while the GDP bound form of CDG01 does not associate with PAK protein in a pull down assay.
For product Datasheets and MSDSs please click on the PDF links below. For additional information, click on the FAQs tab above or contact our Technical Support department at tservice@cytoskeleton.com
Product Description
MSDS
Datasheet
Cat #
Cdc42 protein: GSt tagged: human wid type
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CDG01
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Lesser, C. F. and Miller, S. I. (2001). Expression of microbial virulence proteins in Saccharomyces cerevisiae models mammalian infection. EMBO J. 20, 1840-1849.
Slater, S. J., Seiz, J. L., Stagliano, B. A. and Stubbs, C. D. (2001). Interaction of protein kinase C isozymes with Rho GTPases. Biochemistry 40, 4437-4445.
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