Three classes of proteins tightly regulate small G-proteins: Guanine Nucleotide Exchange Factors (GEFs), GTPase-Activating Proteins (GAPs), and Guanine Nucleotide Dissociation Inhibitors (GDIs).

GEFs activate small GTPases by facilitating the exchange of GDP for GTP.
GAPs inactivate GTPases by accelerating the hydrolysis of GTP to GDP
GDIs maintain GTPases in their inactive GDP-bound state by preventing GDP dissociation.

Cyto offers a range of in vitro assay kits for quantitatively measuring GEF and GAP activity. The kits are ideal for biochemical characterization and high-throughput drug discovery screening. Additionally, we offer a RhoA ELISA assay for the specific detection and quantification of total RhoA.

Three classes of proteins tightly regulate small G-proteins: Guanine Nucleotide Exchange Factors (GEFs), GTPase-Activating Proteins (GAPs), and Guanine Nucleotide Dissociation Inhibitors (GDIs).

GEFs activate small GTPases by facilitating the exchange of GDP for GTP.
GAPs inactivate GTPases by accelerating the hydrolysis of GTP to GDP
GDIs maintain GTPases in their inactive GDP-bound state by preventing GDP dissociation.

Small G-protein GEF/GAP & ELISA kits

Three classes of proteins tightly regulate small G-proteins: Guanine Nucleotide Exchange Factors (GEFs), GTPase-Activating Proteins (GAPs), and Guanine Nucleotide Dissociation Inhibitors (GDIs).

GEFs activate small GTPases by facilitating the exchange of GDP for GTP.
GAPs inactivate GTPases by accelerating the hydrolysis of GTP to GDP
GDIs maintain GTPases in their inactive GDP-bound state by preventing GDP dissociation.

Three classes of proteins tightly regulate small G-proteins: Guanine Nucleotide Exchange Factors (GEFs), GTPase-Activating Proteins (GAPs), and Guanine Nucleotide Dissociation Inhibitors (GDIs).

GEFs activate small GTPases by facilitating the exchange of GDP for GTP.
GAPs inactivate GTPases by accelerating the hydrolysis of GTP to GDP
GDIs maintain GTPases in their inactive GDP-bound state by preventing GDP dissociation.