Cytoskeleton's Small G-protein Activation Assays measure the GTP-bound form of the protein from a cell or tissue extract.
This table outlines some important considerations when deciding which GTPase activation assay is right for you and summarizes what Cytoskeleton feels will be key decision points when choosing between pull-downs and G-LISAs as a means of measuring activation of small G-proteins. For more information and help with deciding which of Cytoskeleton’s activation assays are right for you, please check out the informational links on this page or contact our technical support team at firstname.lastname@example.org or 303-322-2254, ext 316.
Traditional pull-down assays use methods familiar to most labs and therefore provide a familiar point of entry.
The G-LISA assay is simple to use and has many advantages that should be considered (see below for comparisons).
Consider the equipment requirements prior to deciding upon an assay format.
Starting material required per assay
300 - 2000 µg per assay
If starting material is limited, e.g. primary cells, the G-LISA assay is required.
5 – 50 µg per assay
Cost issues to consider:
Cost per assay: The G-LISA is the best value per assay.
Cost per kit: The traditional pull-down assay starter kits generally provide the lowest cost per kit. NOTE: Consider the combo kit as a cost effective choice.
Available in 96 well format. Each well can be broken off and used individually.
Centrifugation steps and western blot analysis makes this assay most suitable for low sample numbers (≤10).
<10 samples: either assay
>10 samples: G-LISA recommended
NOTE: Dose responses and time courses are often recommended and will greatly increase the number of samples being analyzed.
Amenable to high sample numbers (>10) or dose response/time-course analyses. Wells can be detached and used individually.
Quantification of results
Western blot quantification has a very narrow linear range. Also, multiple manipulations of the samples result in a somewhat variable assay.
G-LISA assays are recommended for simple, reproducible quantification of results.
Provides a simple numeric readout
Reagents required that are not in the kits
The G-LISA assay provides all reagents necessary to perform 96 activation assays. Wells can be detached and used individually.
Cytoskeleton's Activation Assays have been cited hundreds of times over the past two decades. Example citations for RhoA G-LISA kit, BK124, is shown, but more citations are available on individual product pages. Please use the "Citations" tab on each individual product page.
|Swaminathan, Bhairavi et al.||Endothelial Notch signaling directly regulates the small GTPase RND1 to facilitate Notch suppression of endothelial migration||Scientific Reports||2022||ISSN 2045-2322|
|Nath, Anu S. et al.||Modulation of the cell membrane lipid milieu by peroxisomal β-oxidation induces Rho1 signaling to trigger inflammatory responses||Cell Reports||2022||ISSN 2211-1247|
|Shoda, Tetsuo et al.||Desmoplakin and periplakin genetically and functionally contribute to eosinophilic esophagitis||Nature Communications||2021||ISSN 2041-1723|
|Zhou, Qun et al.||Inflammatory Immune Cytokine TNF-α Modulates Ezrin Protein Activation via FAK/RhoA Signaling Pathway in PMVECs Hyperpermeability||Frontiers in Pharmacology||2021||ISSN 1663-9812|
|Mao, De Yu et al.||CLIC1 and CLIC4 mediate endothelial S1P receptor signaling to facilitate Rac1 and RhoA activity and function||Science Signaling||2021||ISSN 1937-9145|
|Ma, Yuanyuan et al.||Ror2-mediated non-canonical Wnt signaling regulates Cdc42 and cell proliferation during tooth root development||Development (Cambridge)||2021||ISSN 1477-9129|
|Gurusamy, Malarvizhi et al.||G-protein-coupled receptor P2Y10 facilitates chemokine-induced CD4 T cell migration through autocrine/paracrine mediators||Nature Communications||2021||ISSN 2041-1723|
|Jozic, Ivan et al.||Glucocorticoid-mediated induction of caveolin-1 disrupts cytoskeletal organization, inhibits cell migration and re-epithelialization of non-healing wounds||Communications Biology||2021||ISSN 2399-3642|
|Rong, Zhouyi et al.||Activation of FAK/Rac1/Cdc42-GTPase signaling ameliorates impaired microglial migration response to Aβ42 in triggering receptor expressed on myeloid cells 2 loss-of-function murine models||FASEB Journal||2020||ISSN 1530-6860|
|Lachowski, Dariusz et al.||G Protein-Coupled Estrogen Receptor Regulates Actin Cytoskeleton Dynamics to Impair Cell Polarization||Frontiers in Cell and Developmental Biology||2020||ISSN 2296-634X|
|Hasan, Wan Nuraini Wan et al.||Annatto-derived tocotrienol promotes mineralization of MC3T3-E1 cells by enhancing BMP-2 protein expression via inhibiting RhoA activation and HMG-CoA reductase gene expression||Drug Design, Development and Therapy||2020||ISSN 1177-8881|
|Krueger, Irena et al.||Reelin amplifies glycoprotein VI activation and alphaiib beta3 integrin outside-in signaling via PLC Gamma 2 and Rho GTPases||Arteriosclerosis, Thrombosis, and Vascular Biology||2020||ISSN 1524-4636|
|Porter, Lauren et al.||SUN1/2 Are Essential for RhoA/ROCK-Regulated Actomyosin Activity in Isolated Vascular Smooth Muscle Cells||Cells||2020||ISSN 2073-4409|
|Fox, Megan E. et al.||Dendritic remodeling of D1 neurons by RhoA/Rho-kinase mediates depression-like behavior||Molecular Psychiatry||2020||ISSN 1476-5578|
|Bhosle, Vikrant K. et al.||SLIT2/ROBO1-signaling inhibits macropinocytosis by opposing cortical cytoskeletal remodeling||Nature Communications||2020||ISSN 2041-1723|
|Salgado-Lucio, Monica L. et al.||FAK regulates actin polymerization during sperm capacitation via the ERK2/GEF-H1/RhoA signaling pathway||Journal of Cell Science||2020||ISSN 1477-9137|
|Majolée, Jisca et al.||CSN5 inhibition triggers inflammatory signaling and Rho/ROCK-dependent loss of endothelial integrity||Scientific Reports||2019||ISSN 2045-2322|
|Dias Gomes, Martim et al.||Polarity signaling ensures epidermal homeostasis by coupling cellular mechanics and genomic integrity||Nature Communications||2019||ISSN 2041-1723|
|Li, Xu et al.||A positive feedback loop of profilin-1 and RhoA/ROCK1 promotes endothelial dysfunction and oxidative stress||Oxidative Medicine and Cellular Longevity||2018||ISSN 1942-0994|
|Kapfhamer, Joshua et al.||The Gα q/11 -provoked induction of Akr1c18 in murine luteal cells is mediated by phospholipase C||Molecular and Cellular Endocrinology||2018||ISSN 1872-8057|
|Sun, Xiao Qing et al.||Total flavones of Rhododendron simsii Planch flower protect isolated rat heart from ischaemia-reperfusion injury and its mechanism of UTR-RhoA-ROCK pathway inhibition||Journal of Pharmacy and Pharmacology||2018||ISSN 2042-7158|
|Santhana Kumar, Karthiga et al.||TGF-β Determines the Pro-migratory Potential of bFGF Signaling in Medulloblastoma||Cell Reports||2018||ISSN 2211-1247|
|Tagashira, Toru et al.||Afadin facilitates vascular endothelial growth factor-induced network formation and migration of vascular endothelial cells by inactivating Rho-associated kinase through ARHGAP29||Arteriosclerosis, Thrombosis, and Vascular Biology||2018||ISSN 1524-4636|
|Ng, Tze Hann et al.||The Rho signalling pathway mediates the pathogenicity of AHPND-causing V. parahaemolyticus in shrimp||Cellular Microbiology||2018||ISSN 1462-5822|
|Vautrin-Glabik, Alexia et al.||IP3R3 silencing induced actin cytoskeletal reorganization through ARHGAP18/RhoA/mDia1/FAK pathway in breast cancer cell lines||Biochimica et Biophysica Acta - Molecular Cell Research||2018||ISSN 1879-2596|
|Schillaci, Odessa et al.||Exosomes from metastatic cancer cells transfer amoeboid phenotype to non-metastatic cells and increase endothelial permeability: Their emerging role in tumor heterogeneity||Scientific Reports||2017||ISSN 2045-2322|
|Kempf, Anissa et al.||Control of Cell Shape, Neurite Outgrowth, and Migration by a Nogo-A/HSPG Interaction||Developmental Cell||2017||ISSN 1878-1551|
|Yu, Yonghao et al.||Hydrogen-rich medium ameliorates lipopolysaccharide-induced barrier dysfunction via rhoa-mdia1 signaling in caco-2 cells||Shock||2016||ISSN 1540-0514|
|Lu, Wen Juan et al.||Senescence Mediated by p16INK4a Impedes Reprogramming of Human Corneal Endothelial Cells into Neural Crest Progenitors||Scientific Reports||2016||ISSN 2045-2322|
|Rom, Slava et al.||PARP inhibition in leukocytes diminishes inflammation via effects on integrins/cytoskeleton and protects the blood-brain barrier||Journal of Neuroinflammation||2016||ISSN 1742-2094|
|Xiao, Bin et al.||Extracellular translationally controlled tumor protein promotes colorectal cancer invasion and metastasis through Cdc42/JNK/ MMP9 signaling||Oncotarget||2016||ISSN 1949-2553|
|Jackson, William D. et al.||Very-low and low-density lipoproteins induce neutral lipid accumulation and impair migration in monocyte subsets||Scientific Reports||2016||ISSN 2045-2322|
|López-Posadas, Rocío et al.||Rho-A prenylation and signaling link epithelial homeostasis to intestinal inflammation||Journal of Clinical Investigation||2016||ISSN 1558-8238|
|Liu, Chunqiao et al.||A secreted WNT-ligand-binding domain of FZD5 generated by a frameshift mutation causes autosomal dominant coloboma||Human Molecular Genetics||2016||ISSN 1460-2083|
|Tang, Xiao et al.||HCLOCK Causes Rho-Kinase-Mediated Endothelial Dysfunction and NF-κ B-Mediated Inflammatory Responses||Oxidative Medicine and Cellular Longevity||2015||ISSN 1942-0994|
|Cascio, Graciela et al.||CXCL12 Regulates through JAK1 and JAK2 Formation of Productive Immunological Synapses||The Journal of Immunology||2015||ISSN 0022--1767|
|Manukyan, Arkadi et al.||A complex of p190RhoGAP-A and anillin modulates RhoA-GTP and the cytokinetic furrow in human cells||Journal of Cell Science||2015||ISSN 1477-9137|
|Rom, Slava et al.||The dual action of poly(ADP-ribose) polymerase -1 (PARP-1) inhibition in HIV-1 infection: HIV-1 ltr inhibition and diminution in Rho GTPase activity||Frontiers in Microbiology||2015||ISSN 1664-302X|
|Freeman, Spencer A. et al.||Toll-like receptor ligands sensitize B-cell receptor signalling by reducing actin-dependent spatial confinement of the receptor||Nature Communications||2015||ISSN 2041-1723|
|Rom, Slava et al.||Poly(ADP-ribose) polymerase-1 inhibition in brain endothelium protects the blood-brain barrier under physiologic and neuroinflammatory conditions||Journal of Cerebral Blood Flow and Metabolism||2015||ISSN 1559-7016|
|Ochoa-Alvarez, Jhon A. et al.||Antibody and lectin target podoplanin to inhibit oral squamous carcinoma cell migration and viability by distinct mechanisms||Oncotarget||2015||ISSN 1949-2553|
|Skrbic, Biljana et al.||Lack of collagen VIII reduces fibrosis and promotes early mortality and cardiac dilatation in pressure overload in mice||Cardiovascular Research||2015||ISSN 1755-3245|
|Aifuwa, Ivie et al.||Senescent stromal cells induce cancer cell migration via inhibition of RhoA/ROCK/myosin-based cell contractility||Oncotarget||2015||ISSN 1949-2553|
|Zhu, Ying Ting et al.||Knockdown of both p120 catenin and kaiso promotes expansion of human corneal endothelial monolayers via rhoa-rock-noncanonical BMP-NFκB pathway||Investigative Ophthalmology and Visual Science||2014||ISSN 1552-5783|
|Chen, Xiaofei et al.||The TMEFF2 tumor suppressor modulates integrin expression, RhoA activation and migration of prostate cancer cells||Biochimica et Biophysica Acta - Molecular Cell Research||2014||ISSN 1879-2596|
|Herr, Michael J. et al.||Tetraspanin CD9 regulates cell contraction and actin arrangement via RhoA in human vascular smooth muscle cells||PLoS ONE||2014||ISSN 1932-6203|
|Biechler V., Stefanie V. et al.||The impact of flow-induced forces on the morphogenesis of the outflow tract||Frontiers in Physiology||2014||ISSN 1664-042X|
|Zhang, Di et al.||Co-expression of delta-catenin and RhoA is significantly associated with a malignant lung cancer phenotype||International Journal of Clinical and Experimental Pathology||2014||ISSN 1936-2625|
|Mackay, Joanna L. et al.||Simultaneous and independent tuning of RhoA and Rac1 activity with orthogonally inducible promoters||Integrative Biology (United Kingdom)||2014||ISSN 1757-9708|
|Beveridge, Ryan D. et al.||The leukemia-associated Rho guanine nucleotide exchange factor LARG is required for efficient replication stress signaling||Cell Cycle||2014||ISSN 1551-4005|
|Ahn, Bum Ju et al.||Ninjurin1 enhances the basal motility and transendothelial migration of immune cells by inducing protrusive membrane dynamics||Journal of Biological Chemistry||2014||ISSN 1083-351X|
|Papke, Christina L. et al.||Smooth muscle hyperplasia due to loss of smooth muscle α-actin is driven by activation of focal adhesion kinase, altered p53 localization and increased levels of platelet-derived growth factor receptor-β||Human Molecular Genetics||2013||ISSN 0964-6906|
|Kanazawa, Yasushi et al.||The Rho-kinase inhibitor fasudil restores normal motor nerve conduction velocity in diabetic rats by assuring the proper localization of adhesion-related molecules in myelinating Schwann cells||Experimental neurology||2013||ISSN 1090--2430|
|Tan, Hong et al.||Fluid flow forces and rhoA regulate fibrous development of the atrioventricular valves||Developmental Biology||2013||ISSN 1095-564X|
|DiScipio, Richard G. et al.||Complement C3a signaling mediates production of angiogenic factors in mesenchymal stem cells||Journal of Biomedical Science and Engineering||2013||ISSN 1937--6871|
|Kalia, Manjula et al.||Japanese Encephalitis Virus Infects Neuronal Cells through a Clathrin-Independent Endocytic Mechanism||Journal of Virology||2013||ISSN 0022--538X|
|Yang, Jian et al.||Inhibition of farnesyl pyrophosphate synthase attenuates angiotensin II-induced cardiac hypertrophy and fibrosis in vivo||International Journal of Biochemistry and Cell Biology||2013||ISSN 1357-2725|
|Rom, Slava et al.||Selective activation of cannabinoid receptor 2 in leukocytes suppresses their engagement of the brain endothelium and protects the blood-brain barrier||American Journal of Pathology||2013||ISSN 0002-9440|
|Dubash, Adi D. et al.||The GEF Bcr activates RhoA/MAL signaling to promote keratinocyte differentiation via desmoglein-1||Journal of Cell Biology||2013||ISSN 0021-9525|
|Elali, Ayman et al.||Liver X receptor activation enhances blood-brain barrier integrity in the ischemic brain and increases the abundance of ATP-binding cassette transporters ABCB1 and ABCC1 on brain capillary cells||Brain Pathology||2012||ISSN 1015-6305|
|Zhu, Ying Ting et al.||Nuclear p120 catenin unlocks mitotic block of contactinhibited human corneal endothelial monolayers without disrupting adherent junctions||Journal of Cell Science||2012||ISSN 0021-9533|
|Greco, Carolina M. et al.||Chemotactic effect of prorenin on human aortic smooth muscle cells: a novel function of the (pro)renin receptor||Cardiovascular Research||2012||ISSN 0008--6363|
|Dhaliwal, Anandika et al.||Cellular Cytoskeleton Dynamics Modulates Non-Viral Gene Delivery through RhoGTPases||2012||PMID 22509380|
|Ridgway, Lon D. et al.||Heparanase-induced GEF-H1 signaling regulates the cytoskeletal dynamics of brain metastatic breast cancer cells||Molecular Cancer Research||2012||ISSN 1541-7786|
|Chen, Si Meng et al.||Inhibition of tumor cell growth, proliferation and migration by X-387, a novel active-site inhibitor of mTOR||Biochemical Pharmacology||2012||ISSN 0006-2952|
|Chen, Guang et al.||Inhibition of chemokine (CXC motif) ligand 12/chemokine (CXC motif) receptor 4 axis (CXCL12/CXCR4)-mediated cell migration by targeting mammalian target of rapamycin (mTOR) pathway in human gastric carcinoma cells (Journal of Biological Chemistry (2012) 2||Journal of Biological Chemistry||2012||ISSN 0021-9258|
|Howe, Grant A. et al.||RhoB controls endothelial cell morphogenesis in part via negative regulation of RhoA||Vascular Cell||2012||ISSN 2045--824X|
|Yang, Seungwon et al.||The RhoA-ROCK-PTEN pathway as a molecular switch for anchorage dependent cell behavior||Biomaterials||2012||ISSN 1878--5905|
|Garrido‐Gómez, Tamara et al.||Annexin A2 is critical for embryo adhesiveness to the human endometrium by RhoA activation through F‐actin regulation||The FASEB Journal||2012||ISSN 0892--6638|
|Zhou, Zhigang et al.||HSV-mediated gene transfer of C3 transferase inhibits Rho to promote axonal regeneration||Experimental Neurology||2012||ISSN 0014--4886|
|McCoy, Kelly L. et al.||Protease-activated receptor 1 (PAR1) coupling to G(q/11) but not to G(i/o) or G(12/13) is mediated by discrete amino acids within the receptor second intracellular loop||Cellular signalling||2012||ISSN 1873--3913|
|Ramseyer, Vanesa D. et al.||Tumor necrosis factor α decreases nitric oxide synthase type 3 expression primarily via Rho/Rho kinase in the thick ascending limb||Hypertension (Dallas, Tex. : 1979)||2012||ISSN 1524--4563|
|Takefuji, Mikito et al.||G13-mediated signaling pathway is required for pressure overload-induced cardiac remodeling and heart failure||Circulation||2012||ISSN 0009-7322|
|Jin, Wanzhu et al.||Increased SRF transcriptional activity in human and mouse skeletal muscle is a signature of insulin resistance||Journal of Clinical Investigation||2011||ISSN 0021-9738|
|Aguilar, Hector N. et al.||Phos-tag-based analysis of myosin regulatory light chain phosphorylation in human uterine myocytes||PLoS ONE||2011||ISSN 1932-6203|
|Mammoto, Tadanori et al.||Mechanochemical Control of Mesenchymal Condensation and Embryonic Tooth Organ Formation||Developmental Cell||2011||ISSN 1534-5807|
|Ganguly, Riya et al.||Adiponectin Increases LPL Activity via RhoA/ROCK-Mediated Actin Remodelling in Adult Rat Cardiomyocytes||Endocrinology||2011||ISSN 0013--7227|
|Musso, Alessandra et al.||Relevance of the mevalonate biosynthetic pathway in the regulation of bone marrow mesenchymal stromal cell-mediated effects on T-cell proliferation and B-cell survival||Haematologica||2011||ISSN 1592--8721|
|Yang, Enyue et al.||Fluoride induces vascular contraction through activation of RhoA/Rho kinase pathway in isolated rat aortas||Environmental toxicology and pharmacology||2010||ISSN 1872--7077|
|Ridgway, Lon D. et al.||Modulation of GEF-H1 Induced Signaling by Heparanase in Brain Metastatic Melanoma Cells||Journal of cellular biochemistry||2010||ISSN 0730-2312|
|Nini, Lylia et al.||Accurate and reproducible measurements of RhoA activation in small samples of primary cells||Analytical biochemistry||2010||ISSN 1096--0309|
|Rapier, Rebecca et al.||The extracellular matrix microtopography drives critical changes in cellular motility and Rho A activity in colon cancer cells||Cancer Cell International||2010||ISSN 1475-2867|
|Lichtenstein, Mathieu P. et al.||Secretase-independent and RhoGTPase/PAK/ERK-dependent regulation of cytoskeleton dynamics in astrocytes by NSAIDs and derivatives||Journal of Alzheimer's disease : JAD||2010||ISSN 1875--8908|
|Romero, Ana M. et al.||Chronic ethanol exposure alters the levels, assembly, and cellular organization of the actin cytoskeleton and microtubules in hippocampal neurons in primary culture||Toxicological Sciences||2010||ISSN 1096-6080|
|Hammar, Eva et al.||Role of the Rho-ROCK (Rho-Associated Kinase) Signaling Pathway in the Regulation of Pancreatic β-Cell Function||Endocrinology||2009||ISSN 0013--7227|
|Liu, Yang et al.||Ablation of p120-catenin enhances invasion and metastasis of human lung cancer cells||Cancer Science||2009||ISSN 1347-9032|
|Seifert, Jennifer L. et al.||Differential activation of Rac1 and RhoA in neuroblastoma cell fractions||Neuroscience letters||2009||ISSN 0304--3940|
|Chastre, Eric et al.||TRIP6, a novel molecular partner of the MAGI-1 scaffolding molecule, promotes invasiveness||The FASEB Journal||2009||ISSN 1530--6860|
|Mercer, Jason et al.||Vaccinia virus uses macropinocytosis and apoptotic mimicry to enter host cells||Science (New York, N.Y.)||2008||ISSN 1095--9203|
|Gien, Jason et al.||Chronic intrauterine pulmonary hypertension increases endothelial cell Rho kinase activity and impairs angiogenesis in vitro||American Journal of Physiology - Lung Cellular and Molecular Physiology||2008||ISSN 1040-0605|
|Ramirez, Servio H. et al.||Activation of Peroxisome Proliferator-Activated Receptor γ (PPARγ) Suppresses Rho GTPases in Human Brain Microvascular Endothelial Cells and Inhibits Adhesion and Transendothelial Migration of HIV-1 Infected Monocytes||The Journal of Immunology||2008||ISSN 0022--1767|
|Korobova, Farida et al.||Arp2/3 complex is important for filopodia formation, growth cone motility, and neuritogenesis in neuronal cells||Molecular biology of the cell||2008||ISSN 1939--4586|
|Moore, Simon W. et al.||Rho inhibition recruits DCC to the neuronal plasma membrane and enhances axon chemoattraction to netrin 1||Development||2008||ISSN 0950--1991|
|Kinoshita, Nagatoki et al.||Apical Accumulation of Rho in the Neural Plate Is Important for Neural Plate Cell Shape Change and Neural Tube Formation||Molecular Biology of the Cell||2008||ISSN 1059-1524|
|Sequeira, Linda et al.||Rho GTPases in PC-3 prostate cancer cell morphology, invasion and tumor cell diapedesis||Clinical & experimental metastasis||2008||ISSN 0262--0898|
|Schreibelt, Gerty et al.||Reactive oxygen species alter brain endothelial tight junction dynamics via RhoA, PI3 kinase, and PKB signaling||FASEB journal : official publication of the Federation of American Societies for Experimental Biology||2007||ISSN 1530--6860|
|Keely, Patricia J. et al.||Investigating integrin regulation and signaling events in three-dimensional systems||Methods in enzymology||2007||ISSN 0076--6879|
|Tanaka, Shigeru et al.||Neural expression of G protein-coupled receptors GPR3, GPR6, and GPR12 up-regulates cyclic AMP levels and promotes neurite outgrowth||The Journal of biological chemistry||2007||ISSN 0021--9258|
|Rupp, Paul A. et al.||A role for RhoA in the two-phase migratory pattern of post-otic neural crest cells||Developmental Biology||2007||ISSN 0012-1606|
|Bradley, William D. et al.||Integrin Signaling through Arg Activates p190RhoGAP by Promoting Its Binding to p120RasGAP and Recruitment to the Membrane||Molecular Biology of the Cell||2006||ISSN 1059-1524|
Answer 1: Cytoskeleton, Inc. offers a large number of G-LISA and traditional pull-down activation assays to study the biology and biochemistry of small G-proteins. Our G-LISA activation assays provide an improved method of measuring the activity of small G-proteins utilizing a simple and quick protocol in 96-well format to provide extremely accurate results. To complement our G-LISA line of activation assays, we also offer the most efficiently designed and complete traditional pull-down activation assays available.
Based on enzyme-linked immunosorbent assay (ELISA) technology, our G-LISA activation assays utilize a 96 well format with 12 x 8 well strips that provide the flexibility to run 1 to 96 wells (each well is a condition/treatment) at one time. This flexibility is especially important if any concentration, dose or time course analyses will be performed. Pull-downs are limited to the number of wells in each gel (usually 10-15) that will be run. The G-LISA assays are also more accurate and quantitative than pull-downs with greater sensitivity while using less material per well. The isotype specificity depends on the antibodies being used to capture and visualize the small G-proteins. We have designed both types of activation assays to specifically target RhoA and Rac1. Each of these assays can be easily modified to study RhoB and RhoC or Rac2 and Rac3, respectively. We also offer activation assays for Cdc42, Ras, and RalA. For a quick “look and see” experiment with a few treatment conditions (one drug concentration and time point), pull-downs are convenient. Pull-downs also utilize methods that are very familiar to most labs and so offer a convenient entry point for small G-protein activation assays. Besides the standard sized pull-down kits (50-80 assays, depending on protein), we also offer a starter-sized kit (20 assays) for RhoA, Rac1, Cdc42, and Ras. To screen for multiple small G-proteins, we recommend the Combo starter pull-down kit which provides 10 assays each for RhoA, Rac1, and Cdc42.
For an interactive guide to help choose the activation assay that is right for you, click here.
Answer 2:Yes, the G-LISA activation assays are well-cited in the scientific literature. Please see the “Citations” tab above for links to some current citations for our RhoA, Rac and Cdc42 G-LISA activation assays.
G-LISA Arf1 Activation Assay Biochem Kit (Colorimetric Based)
G-LISA Cdc42 Activation Assay Biochem Kit (Colorimetric format)
G-LISA Cdc42 Activation Assay Biochem Kit (Colorimetric format)
G-LISA RhoA Activation Assay Biochem Kit (Colorimetric format)