Protein Assays

Developed by scientists at Cytoskeleton, the Advanced Protein Assay (Cat. # ADV01) and the Precision Red Advanced Protein Assay (cat. # ADV02) are highly recommended for measuring purified protein and total cell protein alike. The advantages of ADV01 are the low detection limit (0.25 µg protein/ml sample) and the highly similar readings between the same amounts of different purified proteins. ADV02 is less sensitive but more compatible with detergents, and retains the same similarity between different purified proteins. In comparison to the Bradford assay these assays read different purified proteins with two fold greater accuracy which allows the user to rely on the simple A = Ecl  formula to convert the OD into a real protein concentration.

adv01-sds

For protein assay protocols, click here

For more information view ours datasheets, or contact Technical Support at tservice@cytoskeleton.com .

Protein Assay Product Citations

AuthorTitleJournalYearArticle Link
Velázquez-Vega, Luis E. et al.Efficacy and delivery strategies of the dual Rac/Cdc42 inhibitor MBQ-167 in HER2 overexpressing breast cancerTranslational Oncology2024
Cruz-Nova, Pedro et al.131I-C19 Iodide Radioisotope and Synthetic I-C19 Compounds as K-Ras4B–PDE6δ Inhibitors: A Novel Approach against Colorectal Cancer—Biological Characterization, Biokinetics and DosimetryMolecules2022
Hassin, Ori et al.Different hotspot p53 mutants exert distinct phenotypes and predict outcome of colorectal cancer patientsNature Communications 2022 13:12022
Xu, Weiyi et al.Paxillin promotes breast tumor collective cell invasion through maintenance of adherens junction integrityMolecular Biology of the Cell2022
Xu, Weiyi et al.Paxillin promotes breast tumor collective cell invasion through maintenance of adherens junction integrityMolecular biology of the cell2022
Shields, Mario A. et al.Gα13 loss in Kras/Tp53 mouse model of pancreatic tumorigenesis promotes tumors susceptible to rapamycinCell Reports2022
Datta, Gaurav et al.Endolysosome Localization of ERα Is Involved in the Protective Effect of 17α-Estradiol against HIV-1 gp120-Induced Neuronal InjuryThe Journal of neuroscience : the official journal of the Society for Neuroscience2021
Hryniewicz-Jankowska, Anita et al.Article the effect of neddylation inhibition on inflammation-induced mmp9 gene expression in esophageal squamous cell carcinomaInternational Journal of Molecular Sciences2021
Le, Anh Hoang et al.Cyri-a limits invasive migration through macropinosome formation and integrin uptake regulationJournal of Cell Biology2021
Nehme, Jamil et al.High dietary protein and fat contents exacerbate hepatic senescence and SASP in miceFEBS Journal2021
Liao, Liang et al.Disrupting RhoA activity by blocking Arhgef3 expression mitigates microglia-induced neuroinflammation post spinal cord contusionJournal of Neuroimmunology2021
Dolmatova, Elena V et al.Endothelial Poldip2 regulates sepsis-induced lung injury via Rho pathway activationCardiovascular Research2021
Rojas-Colón, Luis A. et al.4R-cembranoid confers neuroprotection against LPS-induced hippocampal inflammation in miceJournal of Neuroinflammation2021
Bordeleau, Francois et al.Tissue transglutaminase 2 regulates tumor cell tensional homeostasis by increasing contractilityJournal of Cell Science2020
Papalazarou, Vassilis et al.The creatine–phosphagen system is mechanoresponsive in pancreatic adenocarcinoma and fuels invasion and metastasisNature Metabolism2020
Dias Gomes, Martim et al.Polarity signaling ensures epidermal homeostasis by coupling cellular mechanics and genomic integrityNature Communications2019
Stephens, Matthew et al.Mesenteric lymphatic alterations observed during DSS induced intestinal inflammation are driven in a TLR4-PAMP/DAMP discriminative mannerFrontiers in Immunology2019
Cederquist, Gustav Y. et al.Specification of positional identity in forebrain organoidsNature Biotechnology2019
Tedeschi, Andrea et al.ADF/Cofilin-Mediated Actin Turnover Promotes Axon Regeneration in the Adult CNSNeuron2019
Kikuchi, Daniel S. et al.Poldip2 mediates blood-brain barrier disruption in a model of sepsis-associated encephalopathyJournal of Neuroinflammation2019
Umesalma, Shaikamjad et al.RABL6A inhibits tumor-suppressive PP2A/AKT signaling to drive pancreatic neuroendocrine tumor growthJournal of Clinical Investigation2019
Paonessa, Francesco et al.Microtubules Deform the Nuclear Membrane and Disrupt Nucleocytoplasmic Transport in Tau-Mediated Frontotemporal DementiaCell Reports2019
Juin, Amelie et al.N-WASP Control of LPAR1 Trafficking Establishes Response to Self-Generated LPA Gradients to Promote Pancreatic Cancer Cell MetastasisDevelopmental Cell2019
Cruz-Nova, Pedro et al.The small organic molecule C19 binds and strengthens the KRAS4b-PDEδ complex and inhibits growth of colorectal cancer cells in vitro and in vivo 11 Medical and Health Sciences 1112 Oncology and CarcinogenesisBMC Cancer2018
Casique-Aguirre, Diana et al.KRas4B-PDE6δ complex stabilization by small molecules obtained by virtual screening affects Ras signaling in pancreatic cancer 06 Biological Sciences 0601 Biochemistry and Cell BiologyBMC Cancer2018
McQueeney, Kelley E. et al.Targeting ovarian cancer and endothelium with an allosteric PTP4A3 phosphatase inhibitorOncotarget2018
Zhu, Yili et al.An in vitro Microscopy-based Assay for Microtubule-binding and Microtubule-crosslinking by Budding Yeast Microtubule-associated ProteinBio-Protocol2018
Proto, Jonathan D. et al.Regulatory T Cells Promote Macrophage Efferocytosis during Inflammation ResolutionImmunity2018
Mangiatordi, Giuseppe Felice et al.Automated identification of structurally heterogeneous and patentable antiproliferative hits as potential tubulin inhibitorsChemical Biology and Drug Design2018
Meiring, Joyce C.M. et al.Co-polymers of Actin and Tropomyosin Account for a Major Fraction of the Human Actin CytoskeletonCurrent Biology2018
Alhasani, Reem Hasaballah et al.Gypenosides protect retinal pigment epithelium cells from oxidative stressFood and Chemical Toxicology2018
Patnaik, Sarita Rani et al.RPGR protein complex regulates proteasome activity and mediates store-operated calcium entryOncotarget2018
Borin, Mirta et al.Rac1 activation links tau hyperphosphorylation and Aß dysmetabolism in Alzheimer's diseaseActa Neuropathologica Communications2018
Zhang, Xiaoqing et al.Alterations of MEK1/2-ERK1/2, IFNγ and Smad2/3 associated Signalling pathways during cryopreservation of ASCs affect their differentiation towards VSMC-like cellsStem Cell Research2018
Horita, Henrick et al.Utilizing a comprehensive immunoprecipitation enrichment system to identify an endogenous post-translational modification profile for target proteinsJournal of Visualized Experiments2018
Hendricks, Louie et al.Milk fat globule-epidermal growth factor-factor VIII–derived peptide MSP68 is a cytoskeletal immunomodulator of neutrophils that inhibits Rac1Journal of Surgical Research2017
Huang, Jia Lin et al.Lipoprotein-biomimetic nanostructure enables efficient targeting delivery of siRNA to Ras-activated glioblastoma cells via macropinocytosisNature Communications2017
Albalawi, Aishah et al.Protective effect of carnosic acid against acrylamide-induced toxicity in RPE cellsFood and Chemical Toxicology2017
Mangiatordi, Giuseppe Felice et al.Novel chemotypes targeting tubulin at the colch***** binding site and unbiasing P-glycoproteinEuropean Journal of Medicinal Chemistry2017
Chen, Lihua et al.Smooth muscle-alpha actin inhibits vascular smooth muscle cell proliferation and migration by inhibiting rac1 activityPLoS ONE2016
Zan, G. Y. et al.p38 mitogen-activated protein kinase activation in amygdala mediates κ opioid receptor agonist U50,488H-induced conditioned place aversionNeuroscience2016
Lampi, Marsha C. et al.Simvastatin ameliorates matrix stiffness-mediated endothelial monolayer disruptionPLoS ONE2016
Chen, Weihua et al.High RhoA expression at the tumor front in clinically localized prostate cancer and association with poor tumor differentiationOncology Letters2016
Castillo-Pichardo, Linette et al.Dietary grape polyphenol resveratrol increases mammary tumor growth and metastasis in immunocompromised mice2013
Zilberter, Misha et al.Dietary energy substrates reverse early neuronal hyperactivity in a mouse model of Alzheimer's diseaseJournal of Neurochemistry2013
Takefuji, Mikito et al.RhoGEF12 controls cardiac remodeling by integrating G protein- and integrindependent signaling cascadesJournal of Experimental Medicine2013
Gil-Henn, H. et al.Arg/Abl2 promotes invasion and attenuates proliferation of breast cancer in vivoOncogene2013
Liu, Yao et al.Actin polymerization-dependent increase in synaptic Arc/Arg3.1 expression in the Amygdala is crucial for the expression of aversive memory associated with drug withdrawalJournal of Neuroscience2012
Padró, Teresa et al.LDL-induced impairment of human vascular smooth muscle cells repair function is reversed by HMG-CoA reductase inhibitionPLoS ONE2012
Ray, Anasuya et al.Hedgehog signaling pathway regulates the growth of ovarian cancer spheroid forming cellsInternational Journal of Oncology2011
Hook, Jeff et al.Functional identity of the gamma tropomyosin gene: Implications for embryonic development, reproduction and cell viabilityBioArchitecture2011
Romero, Ana M. et al.Chronic ethanol exposure alters the levels, assembly, and cellular organization of the actin cytoskeleton and microtubules in hippocampal neurons in primary cultureToxicological Sciences2010
Aguilar, Hector N. et al.Quantification of rapid myosin regulatory light chain phosphorylation using high-throughput in-cell western assays: Comparison to western immunoblotsPLoS ONE2010

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