The BlastR™ lysis filtration system allows users to capture proteins from all cellular compartments using a denaturing buffer to obtain a more complete protein profile relative to standard non-denaturing buffers. One significant drawback with denaturing buffers is the viscous genomic DNA contamination that can interfere with protein analysis, migration of proteins on an SDS-PAGE gel, and interference with immunoprecipitation assays.
BlastR™ filters remove viscous genomic DNA from denaturing cell lysis buffer extracts while reducing processing time to less than one minute. The proprietary filtration technology is based on a compressible polyurethane filter with optimal pore size for capturing genomic DNA. The compressible nature of the filter allows for maximal recovery of sample which is usually 90% of the original volume.
Two versions are available: 1) filters plus buffers version (Cat. # BLR01, includes; BlastR™ Lysis Buffer), which was developed as an integrated component of the Signal-Seeker™ PTM detection product line. The BlastR™ lysis buffer was optimized as a "universal" immunoprecipitation buffer for low abundance PTM modified proteins. Additionally, it effectively isolates proteins from all cellular compartments making it useful for western blot applications. 2) Filters only (Cat. # BLR02, which allows the operator to use their own denaturing buffer such as guanidine, urea or high SDS based extraction buffers that usually produce copious viscosity.
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BlastR™ Lysis/Dilution Buffer Kit