Protein Assays

Umeki et al., 2013. Rapid nucleotide exchange renders asp-11 mutant actins resistant to depolymerizing activity of cofilin, leading to dominant toxicity in vivo. J. Biol. Chem. 288, 1739-1749.  

Used to accurately measure purified proteins prior to using in enzyme assays.

Bachran C, Schneider S, Riese SB, Bachran D, Urban R, Schellmann N, Zahn C, Sutherland M, Fuchs H. (2010). A lysine-free mutant of epidermal growth factor as targeting moiety of a targeted toxin. Life Sci. 88, 226-232.

Used to quantitate purified proteins prior to adding to tissue culture cells. 

Precision Red Protein Assay: 1x stock  (Cat. # ADV02)

Smithrithee R. et al. 2015. Human β-defensin-3 increases the expression of interleukin-37 through CCR6 in human keratinocytes. J. Dermatol. Sci. 77, 46-53.

Used to quantify the concentration of total protein in the lysates of keratinocytes incubated with various stimulants or inhibitors to equalize total protein levels before samples were analyzed by Western blotting to examine how these modulators affect expression levels of interleukin-37, a new member of the interleukin-1 family.

Boteanu R.M. et al. 2015. High-mobility group box 1 enhances the inflammatory process in diabetic lung. Arch. Biochem. Biophys. 583, 55-64. 

Used to quantify total protein content of extracts prior to loading on to western blots.

Castillo-Pichardo et al. 2013. Dietary grape polyphenol resveratrol increases mammary tumor growth and metastasis in immunocompromised mice. BMC Complement. Altern. Med. 13:6.
 

Used to quantify the concentration of total proteins from a variety of cell lysates, including MDA-MB-231-derived primary tumors, quiescent MDA-MB-231 or MDA-MB-435 cells treated with resveratrol, or mammary tumors for later analyses by either Western blotting for tumor-promoting molecules or Rac G-LISA activation assays to better understand how resveratrol affects breast cancer.