Live Cell Imaging Reagents

Cytoskeleton offers several reagents for live-cell research including fluorescent proteins, cell permeable protein activators and inhibitors, as well as our recent addition of live cell imaging probes. 

Spirochrome Probes For Bioimaging

Live-cell imaging probes developed by Spirochrome are cell-permeable compounds which stain microtubules (SiR-Tubulin), F-actin (SiR-Actin), Lysosomes (SiR-Lysosome) and chromosomal DNA (SiR-DNA) in living cells. 

Cytoskeleton is excited to introduce the next generation of Spirochrome’s live cell imaging probes - the SPY™ probes. SPY™ probes improve upon the SiR live cell imaging technology while also expanding the fluorophore labeling options for the study of F-actin, microtubules, and DNA in living cells.

MemGlow™ Probes For Bioimaging: From The MemBright Family Of Probes

Cytoskeleton is proud to announce 5 new bright fluorogenic probes to visualize the cellular plasma membrane.  Originally developed as the MemBright probes,  MemGlow™ probes are highly photostable, fluorogenic, and non-toxic to cells.

MemGlow™ probes are highly efficient and allow scientists to visualize cellular nanostructures with nanomolar probe concentrations.

Browse the options below to learn more about these live cell imaging tools.

Cytoskeleton's live cell imaging products have been cited hundreds of times over the past two decades. A select few are described here. More individual product citations listed on each product page.

AuthorTitleJournalYearArticle Link
Baron, Desiree M et al.ALS-associated KIF5A mutations abolish autoinhibition resulting in a toxic gain of function.Cell reports2022ISSN 2211--1247
Lee, Gloria et al.Active cytoskeletal composites display emergent tunable contractility and restructuringSoft Matter2021ISSN 1744-6848
Cheng, Xianrui et al.Xenopus laevis egg extract preparation and live imaging methods for visualizing dynamic cytoplasmic organizationJournal of Visualized Experiments2021ISSN 1940-087X
Goldstein-Levitin, Alina et al.Intracellular functions and motile properties of bi-directional kinesin-5 cin8 are regulated by neck linker dockingeLife2021ISSN 2050-084X
Farhadi, Leila et al.Actin and microtubule crosslinkers tune mobility and control co-localization in a composite cytoskeletal networkSoft Matter2020ISSN 1744-6848
Aher, Amol et al.CLASP Mediates Microtubule Repair by Restricting Lattice Damage and Regulating Tubulin IncorporationCurrent Biology2020ISSN 1879-0445
Nolet, Felix E. et al.Nuclei determine the spatial origin of mitotic waveseLife2020ISSN 2050-084X
Rodríguez-García, Ruddi et al.Mechanisms of Motor-Independent Membrane Remodeling Driven by Dynamic MicrotubulesCurrent Biology2020ISSN 1879-0445
Chen, Keyu et al.Giant ankyrin-B suppresses stochastic collateral axon branching through direct interaction with microtubulesJournal of Cell Biology2020ISSN 1540-8140
Nakos, Konstantinos et al.Septin 2/6/7 complexes tune microtubule plus-end growth and EB1 binding in a concentration- And filament-dependent mannerMolecular Biology of the Cell2019ISSN 1939-4586
Nakos, Konstantinos et al.Regulation of microtubule plus end dynamics by septin 9Cytoskeleton2019ISSN 1949-3592
Guedes-Dias, Pedro et al.Kinesin-3 Responds to Local Microtubule Dynamics to Target Synaptic Cargo Delivery to the PresynapseCurrent Biology2019ISSN 0960-9822
Zhu, Yili et al.An in vitro Microscopy-based Assay for Microtubule-binding and Microtubule-crosslinking by Budding Yeast Microtubule-associated ProteinBio-Protocol2018ISSN 2331--8325
Melo, Esther et al.HtrA1 Mediated Intracellular Effects on Tubulin Using a Polarized RPE Disease ModelEBioMedicine2018ISSN 2352-3964
Aher, Amol et al.CLASP Suppresses Microtubule Catastrophes through a Single TOG DomainDevelopmental Cell2018ISSN 1878-1551
Colin, Alexandra et al.Actin-Network Architecture Regulates Microtubule DynamicsCurrent Biology2018ISSN 0960-9822
McClintock, Mark A. et al.RNA-directed activation of cytoplasmic dynein-1 in reconstituted transport RNPseLife2018ISSN 2050-084X
Fan, Yuanwei et al.The Arabidopsis SPIRAL2 Protein Targets and Stabilizes Microtubule Minus EndsCurrent Biology2018ISSN 0960-9822
Arellano-Santoyo, Hugo et al.A Tubulin Binding Switch Underlies Kip3/Kinesin-8 Depolymerase ActivityDevelopmental Cell2017ISSN 1878-1551
Kandel, Mikhail E. et al.Label-Free Imaging of Single Microtubule Dynamics Using Spatial Light Interference MicroscopyACS Nano2017ISSN 1936-086X
Zhang, Rui et al.Interplay of structure, elasticity, and dynamics in actin-based nematic materialsProceedings of the National Academy of Sciences of the United States of America2017ISSN 1091-6490
Britto, Mishan et al.Schizosaccharomyces pombe kinesin-5 switches direction using a steric blocking mechanismProceedings of the National Academy of Sciences of the United States of America2016ISSN 1091-6490
Taberner, Núria et al.Reconstituting Functional Microtubule-Barrier InteractionsMethods in Cell Biology2014ISSN 0091-679X
DeBerg, Hannah A. et al.Motor domain phosphorylation modulates kinesin-1 transportJournal of Biological Chemistry2013ISSN 0021-9258
Rostovtseva, Tatiana K. et al.Membrane Lipid Composition Regulates Tubulin Interaction with Mitochondrial Voltage-dependent Anion Channel *Journal of Biological Chemistry2012ISSN 0021--9258
Hara, Masatoshi et al.Greatwall kinase and cyclin B-Cdk1 are both critical constituents of M-phase-promoting factorNature Communications2012ISSN 2041-1723
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    ER Flipper-TR Kit, a probe for measuring plasma-membrane tension.

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  2. Exoenzyme C3 transferase protein: His tagged:Clostridium botulinum recombinant CT03
    Exoenzyme C3 transferase protein: His tagged:Clostridium botulinum recombinant
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  3. Fibronectin (Green fluorescent, HiLyte 488) FNR02
    Fibronectin (Green fluorescent, HiLyte 488)
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  4. Flipper-TR Kit for fluorescence cell membrane microscopy CY-SC020
    Flipper-TR Kit, a probe for measuring plasma-membrane tension.
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  5. Lyso Flipper-TR Kit for fluorescence cell membrane microscopy CY-SC022

    Lyso Flipper-TR Kit, a probe for measuring plasma-membrane tension.

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  6. Mito Flipper-TR Kit for fluorescence cell membrane microscopy CY-SC023

    Mito Flipper-TR Kit, a probe for measuring plasma-membrane tension.

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