MemGlow™ Probes for Bioimaging: from the MemBright family of probes

New bright fluorogenic MemGlow™ probes* for cell membranes now available!

Cytoskeleton is proud to announce 3 new bright fluorogenic probes to visualize the cellular plasma membrane.  Originally developed as the MemBright probes,  MemGlow™ probes provide high photostability, exhibit excellent fluorescent quantum yields, and some of them are super-resolution compatible.

MemGlow™ probes are chemically composed of cyanine or BODIPY dyes bearing zwitterionic amphiphilic anchors for enhanced retention in the plasma membrane.

The fluorogenic nature of MemGlow™ probes translates to minimal fluorescent emission until their contact with the plasma membrane elicits integration into the lipid bilayer. This characteristic creates minimal background, bright plasma membrane localization, and a no-wash-required application.

MemGlow™ probes are highly efficient and allow scientists to visualize cellular nanostructures with nanomolar probe concentrations. 

Features and advantages of MemGlow™ probes:

  • Bright and fluorogenic
  • Simple staining protocol and low working concentration
  • Compatible with live and fixed cell staining
  • Compatible with ex vivo and fixed tissue staining 
  • MemGlow 560 and 640 are super-resolution compatible
  • Non-toxic probes permit long-term imaging and re-imaging of live cells
  • Efficient labeling of filopodia and nanotubes at nanomolar concentrations
  • No dye quenching steps required
  • Do not alter sample biology
  • Utilize cyanine or BODIPY dyes with zwitterionic membrane anchor groups
  • Superior to many existing plasma membrane dyes

For an in-depth look at MemGlow™ probes (part of the MemBright family) view their groundbreaking publication by clicking this  link. 

Turn-on mechanism of MemGlow™ probes. MemGlow™ probes are self-quenched nanoparticles until integration with the plasma membrane enables their excitation.