Kits & Assays

Cytoskeleton's Biochem Kits™ are comprehensive kits for assaying different aspects of signal transduction processes and cytoskeletal biochemistry.  These kits help scientists produce publication quality data in a short period of time (Click citation tab above for examples).  These kits come with all the reagents needed for the assay as well as detailed instruction on how to use them, so you will be ready to perform experiments as soon as the kits arrives!

 

For more information about our Biochem Kits please click the About tab above.

Kit-CatImg

About Cytoskeleton's Biochem™ kits

Biochem™ kits are an extremely quick way to become familiar with some of the most useful in vitro and in vivo assays in signal transduction and cytoskeletal research. Each kit is carefully quality controlled and very user friendly and will therefore save time and money even for experienced cytoskeletal researchers. Cytoskeleton provides Biochem kits for actin, tubulin, intermediate filament, small G-protein, signal transduction and molecular motor research.

If you are new to the field of cytoskeletal research, the Biochem™ kits will prove to be an invaluable introductory aid, providing many useful technical tips. For example, if you are new to the field of microtubule research, you may not know that tubulin requires GTP, Mg2+ and >50 mM PIPES for stability and that taxol (a microtubule stabilizing drug) added too quickly will cause aberrant tubulin arrangements to form. If either of these points are overlooked, the results could lead to inappropriate experimental interpretation.Biochem™ kits are designed to overcome this bottleneck in research.

As an example: If you wanted to know whether a specific protein or compound interacts with actin filaments and/or monomer, you could order one of several Biochem kits specializing in actin research:

1) Actin binding protein assay Biochem™ kit (Cat. # BK001 or BK013): This kit provides clear instructions and all the reagents and controls necessary to determine the ability of a given protein to bind to actin filaments. It should be noted that in some cases actin binding proteins have a low affinity for actin filaments. Therefore, it is wise to couple this assay with the Actin polymerization assay Biochem™ kit in order to obtain a more complete profile of your potential actin binding protein.

2) Actin polymerization assay Biochem™ kit (Cat. # BK003): This kit uses modified actin to follow polymerization kinetics. There are many types of actin binding proteins, including nucleating, capping, severing, side-binding and monomer sequestering actin binding proteins. All of these can be characterized by their effect on actin polymerization. Cytoskeleton has adapted this powerful assay into a user friendly Biochem™ kit format.

Biochem™ kits are also a great way to optimize your research time and money. When you buy a Biochem™ kit, you obtain all the necessary proteins, buffers, controls and instructions that you require to perform your research with the highest degree of confidence. Furthermore, Cytoskeleton Inc guarantees that the cost of each kit is substantially less than the sum of the component parts purchased separately.

Many publications cite the use of Cytoskeleton's kits in the Materials and Methods section of papers. Usually the citation is associated with a particular result in the form of a graph or image that helps the authors present their findings. This indicates the utility of the Kits to produce publication quality data in a short timeframe thus helping improve the productivity of your efforts. Example citations for the BK011P kit is shown, but more citations are available on individual product pages.


AuthorTitleJournalYearArticle Link
Kumar N, Manoj et al.Benzothiazole-[1,2,3]triazolo[5,1-a]isoindoles: Synthesis, anticancer activity, bioavailability and in silico studies against Gama-Tubulin proteinJournal of Molecular Structure2022ISSN 0022-2860
Yao, Yongfang et al.Angiogenesis and anti-leukaemia activity of novel indole derivatives as potent colchicine binding site inhibitorsJournal of Enzyme Inhibition and Medicinal Chemistry2022ISSN 1475-6374
Wang, Zhan et al.Design, synthesis and biological evaluation of colchicine glycoconjugates as tubulin polymerization inhibitorsBioorganic and Medicinal Chemistry2022ISSN 1464-3391
Ma, Juan et al.Discovery of Novel 3,4-Dihydro-2(1H)-Quinolinone Sulfonamide Derivatives as New Tubulin Polymerization Inhibitors with Anti-Cancer ActivityMolecules2022ISSN 1420-3049
Würtz, Martin et al.Modular assembly of the principal microtubule nucleator γ-TuRCNature Communications2022ISSN 2041-1723
Liu, Chao et al.Synthesis and biological evaluation of BU-4664L derivatives as potential anticancer agentsBioorganic and Medicinal Chemistry Letters2022ISSN 1464-3405
Wang, Guangcheng et al.Design, synthesis and biological evaluation of novel 2-phenyl-4,5,6,7-tetrahydro-1H-​indole derivatives as potential anticancer agents and tubulin polymerization inhibitorsArabian Journal of Chemistry2022ISSN 1878-5352
Patel, Onisha et al.Structural basis for small molecule targeting of Doublecortin Like Kinase 1 with DCLK1-IN-1Communications Biology2021ISSN 2399-3642
Fareed, Momen R. et al.New multi-targeted antiproliferative agents: Design and synthesis of ic261-based oxindoles as potential tubulin, ck1 and egfr inhibitorsPharmaceuticals2021ISSN 1424-8247
Rahimzadeh Oskuei, Sara et al.Design, synthesis and biological evaluation of novel imidazole-chalcone derivatives as potential anticancer agents and tubulin polymerization inhibitorsBioorganic Chemistry2021ISSN 1090-2120
Liang, Dong et al.Identification of anthelmintic parbendazole as a therapeutic molecule for HNSCC through connectivity map-based drug repositioningActa Pharmaceutica Sinica B2021ISSN 2211-3843
Sun, Chiao Yin et al.LMBD1 protein participates in cell mitosis by regulating microtubule assemblyBiochemical Journal2021ISSN 1470-8728
Wang, Chao et al.Design, synthesis and biological evaluation of 1-Aryl-5-(4-arylpiperazine-1-carbonyl)-1H-tetrazols as novel microtubule destabilizersJournal of Enzyme Inhibition and Medicinal Chemistry2021ISSN 1475-6374
Würtz, Martin et al.Reconstitution of the recombinant human γ-tubulin ring complexOpen Biology2021ISSN 2046-2441
Grohmann, Christoph et al.Preclinical small molecule WEHI-7326 overcomes drug resistance and elicits response in patient-derived xenograft models of human treatment-refractory tumorsCell Death and Disease2021ISSN 2041-4889
Yang, Mei et al.C118P, a novel microtubule inhibitor with anti-angiogenic and vascular disrupting activities, exerts anti-tumor effects against hepatocellular carcinomaBiochemical Pharmacology2021
Lead optimization of novel quinolone chalcone compounds by a structure-activity relationship (SAR) study to increase efficacy and metabolic stability2021Article Link
Shawky, Ahmed M. et al.Novel pyrrolizines bearing 3,4,5-trimethoxyphenyl moiety: design, synthesis, molecular docking, and biological evaluation as potential multi-target cytotoxic agentsJournal of Enzyme Inhibition and Medicinal Chemistry2021ISSN 1475-6374
Woo, Jung AA et al.β-arrestin1 promotes tauopathy by transducing GPCR signaling, disrupting microtubules and autophagyLife science alliance2021ISSN 2575--1077
Liu, Qian et al.Identification of a lathyrane-type diterpenoid EM-E-11-4 as a novel paclitaxel resistance reversing agent with multiple mechanisms of actionAging2020ISSN 1945-4589
Abdel-Rahman, Somaya A. et al.Cyclohepta[ b]thiophenes as Potential Antiproliferative Agents: Design, Synthesis, in Vitro, and in Vivo Anticancer EvaluationACS Pharmacology and Translational Science2020ISSN 2575-9108
Barnes, Natalie G. et al.A 2-step synthesis of Combretastatin A-4 and derivatives as potent tubulin assembly inhibitorsBioorganic and Medicinal Chemistry2020ISSN 1464-3391
Palumbo, Valeria et al.Drosophila Morgana is an Hsp90-interacting protein with a direct role in microtubule polymerizationJournal of Cell Science2020ISSN 1477-9137
Mirzaei, Salimeh et al.Synthesis, structure-activity relationship and molecular docking studies of novel quinoline-chalcone hybrids as potential anticancer agents and tubulin inhibitorsJournal of Molecular Structure2020ISSN 0022-2860
Chen, Shih Yin et al.Exosomal 2′,3′-CNP from mesenchymal stem cells promotes hippocampus CA1 neurogenesis/neuritogenesis and contributes to rescue of cognition/learning deficiencies of damaged brainStem Cells Translational Medicine2020ISSN 2157-6580
Song, Ming Yu et al.Exploring diverse-ring analogues on combretastatin a4 (Ca-4) olefin as microtubule-targeting agentsInternational Journal of Molecular Sciences2020ISSN 1422-0067
Ullah, Imran et al.An Antiparasitic Compound from the Medicines for Malaria Venture Pathogen Box Promotes Leishmania Tubulin PolymerizationACS Infectious Diseases2020ISSN 2373-8227
Tariq, Ammarah et al.In vitro reconstitution of branching microtubule nucleationeLife2020ISSN 2050-084X
Zhernov, Ilia et al.Intrinsically Disordered Domain of Kinesin-3 Kif14 Enables Unique Functional DiversityCurrent Biology2020ISSN 1879-0445
Zdioruk, Mykola et al.A new inhibitor of tubulin polymerization kills multiple cancer cell types and reveals p21-mediated mechanism determining cell death after mitotic catastropheCancers2020ISSN 2072-6694
Skidmore, Lillian et al.ARX788, a site-specific anti-HER2 antibody–drug conjugate, demonstrates potent and selective activity in HER2-low and T-DM1–resistant breast and gastric cancersMolecular Cancer Therapeutics2020ISSN 1538-8514
Wang, Yanming et al.Antibody-drug conjugate using ionized CYS-linker-mmae as the potent payload shows optimal therapeutic safetyCancers2020ISSN 2072-6694
Baker, Stacey J. et al.A Contaminant Impurity, Not Rigosertib, Is a Tubulin Binding AgentMolecular Cell2020ISSN 1097-4164
Morita, Ken et al.Allosteric Activators of Protein Phosphatase 2A Display Broad Antitumor Activity Mediated by Dephosphorylation of MYBL2Cell2020ISSN 1097-4172
Dillon, Gregory M. et al.Acute inhibition of the CNS-specific kinase TTBK1 significantly lowers tau phosphorylation at several disease relevant sitesPLoS ONE2020ISSN 1932-6203
Zhai, Min'an et al.3,5-Diaryl-1H-pyrazolo[3,4-b]pyridines as potent tubulin polymerization inhibitors: Rational design, synthesis and biological evaluationEuropean Journal of Medicinal Chemistry2019ISSN 1768-3254
Su, Wenhui et al.Cdc42 is involved in NC1 peptide-regulated BTB dynamics through actin and microtubule cytoskeletal reorganizationFASEB journal : official publication of the Federation of American Societies for Experimental Biology2019ISSN 1530-6860
Wang, Yanming et al.Novel silyl ether-based acid-cleavable antibody-MMAE conjugates with appropriate stability and efficacyCancers2019ISSN 2072-6694
Cui, Ying Jie et al.Synthesis of novel pyrazole derivatives and their tumor cell growth inhibitory activityMolecules2019ISSN 1420-3049
Shaik, Thoukhir B. et al.Evaluation of Anticancer and Anti-Mitotic Properties of Quinazoline and Quinazolino-Benzothiadiazine DerivativesAnti-Cancer Agents in Medicinal Chemistry2019ISSN 1871-5206
Sonawane, Vinay et al.Cink4T, a quinazolinone-based dual inhibitor of Cdk4 and tubulin polymerization, identified via ligand-based virtual screening, for efficient anticancer therapyEuropean Journal of Medicinal Chemistry2019ISSN 1768-3254
Xia, Xiaoyu et al.Leukemia Cell Cycle Chemical Profiling Identifies the G2-Phase Leukemia Specific Inhibitor Leusin-1ACS Chemical Biology2019ISSN 1554-8937
Behbahani, Fatemeh Shaebani et al.Synthesis and biological evaluation of novel benzo[c]acridine-diones as potential anticancer agents and tubulin polymerization inhibitorsArchiv der Pharmazie2019ISSN 1521-4184
Karimikia, Ehsan et al.Colchicine-like β-acetamidoketones as inhibitors of microtubule polymerization: Design, synthesis and biological evaluation of in vitro anticancer activityIranian Journal of Basic Medical Sciences2019ISSN 2008-3874
Patterson, Jesse C. et al.VISAGE Reveals a Targetable Mitotic Spindle Vulnerability in Cancer CellsCell Systems2019ISSN 2405-4720
Mao, Bai Ping et al.CaMSAP2 is a microtubule minus-end targeting protein that regulates BTB dynamics through cytoskeletal organizationEndocrinology2019ISSN 1945-7170
Eberle-Singh, Jaime A. et al.Effective delivery of a microtubule polymerization inhibitor synergizes with standard regimens in models of pancreatic ductal adenocarcinomaClinical Cancer Research2019ISSN 1557-3265
Lukinavičius, Gražvydas et al.Fluorescent dyes and probes for super-resolution microscopy of microtubules and tracheoles in living cells and tissuesChemical Science2018ISSN 2041-6539
Tian, Zhenhua et al.Biological activity and interaction mechanism of the diketopiperazine derivatives as tubulin polymerization inhibitorsRSC Advances2018ISSN 2046-2069
Simpkins, Scott W. et al.Predicting bioprocess targets of chemical compounds through integration of chemical-genetic and genetic interactionsPLoS Computational Biology2018ISSN 1553-7358
Nixon, Gemma L. et al.Repurposing and reformulation of the antiparasitic agent flubendazole for treatment of cryptococcal meningoencephalitis, a neglected fungal diseaseAntimicrobial Agents and Chemotherapy2018ISSN 1098-6596
Sato-Kaneko, Fumi et al.Discovery of a Novel Microtubule Targeting Agent as an Adjuvant for Cancer ImmunotherapyBioMed Research International2018ISSN 2314-6141
Dilworth, David et al.The prolyl isomerase FKBP25 regulates microtubule polymerization impacting cell cycle progression and genomic stabilityNucleic Acids Research2018ISSN 1362-4962
Huan, L C et al.Exploration of Some Thiazolidine-2, 4-dione and 2-Oxoindoline Derivatives Incorporating 3, 4, 5-Trimethoxybenzyl Moiety as Novel Anticancer AgentsLetters in Drug …2018Article Link
Qi, Jianguo et al.Synthesis and biological evaluation of N-substituted 3-oxo-1,2,3,4-tetrahydro-quinoxaline-6-carboxylic acid derivatives as tubulin polymerization inhibitorsEuropean Journal of Medicinal Chemistry2018ISSN 1768-3254
Senese, Silvia et al.Microtubins: A novel class of small synthetic microtubule targeting drugs that inhibit cancer cell proliferationOncotarget2017ISSN 1949-2553
Wieczorek, Anna et al.Synthesis and evaluation of biological properties of ferrocenyl-podophyllotoxin conjugatesDalton Transactions2017ISSN 1477-9234
Sun, Maolin et al.Synthesis and bioevaluation of N,4-diaryl-1,3-thiazole-2-amines as tubulin inhibitors with potent antiproliferative activityPLoS ONE2017ISSN 1932-6203
Zollo, Massimo et al.PRUNE is crucial for normal brain development and mutated in microcephaly with neurodevelopmental impairmentBrain2017ISSN 1460-2156
Chen, Minfeng et al.Pericyte-targeting prodrug overcomes tumor resistance to vascular disrupting agentsJournal of Clinical Investigation2017ISSN 1558-8238
Tantak, Mukund P. et al.Design and synthesis of bis(indolyl)ketohydrazide-hydrazones: Identification of potent and selective novel tubulin inhibitorsEuropean Journal of Medicinal Chemistry2017ISSN 1768-3254
Xu, Qile et al.Design, synthesis and structure-Activity relationship of 3,6-diaryl-7H-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazines as novel tubulin inhibitorsScientific Reports2017ISSN 2045-2322
Kowalczyk, Karolina et al.Colchicine metallocenyl bioconjugates showing high antiproliferative activities against cancer cell linesDalton Transactions2017ISSN 1477-9234
Wu, Yue et al.Design and synthesis of 5-aryl-4-(4-arylpiperazine-1-carbonyl)-2H-1,2,3-triazole derivatives as colchicine binding site inhibitorsScientific Reports2017ISSN 2045-2322
Fu, Ying et al.The contrasting catalytic efficiency and cancer cell antiproliferative activity of stereoselective organoruthenium transfer hydrogenation catalystsDalton Transactions2016ISSN 1477-9234
Cai, De et al.YSL-12, a novel microtubule-destabilizing agent, exerts potent anti-tumor activity against colon cancer in vitro and in vivoCancer Chemotherapy and Pharmacology2016ISSN 1432-0843
Magalhaes, Luma G. et al.Discovery of a series of acridinones as mechanism-based tubulin assembly inhibitors with anticancer activityPLoS ONE2016ISSN 1932-6203
Hasanpourghadi, Mohadeseh et al.Targeting of tubulin polymerization and induction of mitotic blockage by Methyl 2-(5-fluoro-2-hydroxyphenyl)-1H-benzo[d]imidazole-5-carboxylate (MBIC) in human cervical cancer HeLa cellJournal of Experimental and Clinical Cancer Research2016ISSN 1756-9966
Pearson, Brandon L. et al.Identification of chemicals that mimic transcriptional changes associated with autism, brain aging and neurodegenerationNature Communications2016ISSN 2041-1723
Yoshitake, Jun et al.Modification of tau by 8-nitroguanosine 3′,5′-cyclic monophosphate (8-nitro-cGMP): Effects of nitric oxide-linked chemical modification on tau aggregationJournal of Biological Chemistry2016ISSN 1083-351X
Thapa, Pritam et al.Far-Red Light-Activatable Prodrug of Paclitaxel for the Combined Effects of Photodynamic Therapy and Site-Specific Paclitaxel ChemotherapyJournal of Medicinal Chemistry2016ISSN 1520-4804
Fukuda, Yasunori et al.Tubulin is a molecular target of the Wnt-activating chemical probeBMC Biochemistry2016ISSN 1471-2091
Kamal, Ahmed et al.Design, synthesis and antiproliferative activity of the new conjugates of E7010 and resveratrol as tubulin polymerization inhibitorsOrganic and Biomolecular Chemistry2016ISSN 1477-0520
Fawzy, Iten M. et al.Newly Designed and Synthesized Curcumin Analogs with in vitro Cytotoxicity and Tubulin Polymerization ActivityChemical biology & drug design2015ISSN 1747--0285
Mu, Yan et al.The novel tubulin polymerization inhibitor MHPT exhibits selective anti-tumor activity against rhabdomyosarcoma in vitro and in vivoPLoS ONE2015ISSN 1932-6203
Xu, Qile et al.Synthesis and biological evaluation of 3-alkyl-1,5-diaryl-1H-pyrazoles as rigid analogues of combretastatin A-4 with potent antiproliferative activityPLoS ONE2015ISSN 1932-6203
Seashore-Ludlow, Brinton et al.Harnessing connectivity in a large-scale small-molecule sensitivity datasetCancer Discovery2015ISSN 2159-8290
Remers, William A. et al.Synthesis and Antitumor Activity of Heterocycles Related to CarbendazimJournal of Heterocyclic Chemistry2015ISSN 1943--5193
Sheldon, Jonathon E. et al.Photoswitchable anticancer activity via trans-cis isomerization of a combretastatin A-4 analogOrganic and Biomolecular Chemistry2015ISSN 1477-0520
Calles, Antonio et al.Tivantinib (ARQ 197) efficacy is independent of MET inhibition in non-small-cell lung cancer cell linesMolecular Oncology2015ISSN 1878-0261
Yan, Jun et al.A novel synthetic compound exerts effective anti-tumour activity in vivo via the inhibition of tubulin polymerisation in A549 cellsBiochemical Pharmacology2015ISSN 1873-2968
Wu, Shaoyu et al.Bis-cyclopropane analog of disorazole C1 is a microtubuledestabilizing agent active in ABCB1-overexpressing human colon cancer cellsOncotarget2015ISSN 1949-2553
Shigehiro, Tsukasa et al.Efficient Drug Delivery of Paclitaxel Glycoside: A Novel Solubility Gradient Encapsulation into Liposomes Coupled with Immunoliposomes PreparationPLOS ONE2014ISSN 1932--6203
Choi, Bo Hwa et al.Suprafenacine, an Indazole-Hydrazide Agent, Targets Cancer Cells Through Microtubule DestabilizationPLOS ONE2014ISSN 1932--6203
Senese, S. et al.Chemical dissection of the cell cycle: Probes for cell biology and anti-cancer drug developmentCell Death and Disease2014ISSN 2041-4889
Lutz, Vanessa et al.SAR studies on hydropentalene derivatives - Important core units of biologically active tetramic acid macrolactams and ptychanolidesBioorganic and Medicinal Chemistry2014ISSN 1464-3391
Mei, Mei et al.A new 2α,5α,10β,14β-tetraacetoxy-4(20),11-taxadiene (SIA) derivative overcomes paclitaxel resistance by inhibiting MAPK signaling and increasing paclitaxel accumulation in breast cancer cellsPLoS ONE2014ISSN 1932-6203
Bio, Moses et al.Far-red light activatable, multifunctional prodrug for fluorescence optical imaging and combinational treatmentJournal of Medicinal Chemistry2014ISSN 1520-4804
Mäki-Jouppila, Jenni H.E. et al.Centmitor-1, a novel acridinyl-acetohydrazide, possesses similar molecular interaction field and antimitotic cellular phenotype as rigosertib, on 01910.NaMolecular Cancer Therapeutics2014ISSN 1538-8514
Zhang, Qiu et al.Anti-tumor selectivity of a novel Tubulin and HSP90 dual-targeting inhibitor in non-small cell lung cancer modelsBiochemical Pharmacology2013ISSN 1873-2968
Bio, Moses et al.Site-specific and far-red-light-activatable prodrug of combretastatin A-4 using photo-unclick chemistryJournal of Medicinal Chemistry2013ISSN 0022-2623
Zach, Frank et al.The retinitis pigmentosa 28 protein FAM161A is a novel ciliary protein involved in intermolecular protein interaction and microtubule associationHuman molecular genetics2012ISSN 1460--2083
Sidhaye, Venkataramana K. et al.A Novel Role for Aquaporin-5 in Enhancing Microtubule Organization and StabilityPLOS ONE2012ISSN 1932--6203
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Fitzgerald, Daniel P. et al.TPI-287, a new taxane family member, reduces the brain metastatic colonization of breast cancer cellsMolecular Cancer Therapeutics2012ISSN 1535-7163
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Question 1:  How can Cytoskeleton’s Biochem Kits help me in my research?

Question 2:  How can I use the Biochem Kits to investigate whether my protein interacts with the cytoskeleton or its regulatory components?

 


Question 1: How can Cytoskeleton’s Biochem Kits help me in my research?

Answer 1:Cytoskeleton's Biochem Kits are an extremely quick way to become familiar with some of the most useful in vitro and in vivo assays for assaying different aspects of cytoskeletal biochemistry and signal transduction. These kits come with all the reagents needed for your assay as well as detailed instruction on how to use them, so you will be ready to do your assays as soon as you have the kits!  Each kit is carefully quality controlled and very user friendly and will therefore save time and money even for experienced cytoskeletal researchers.  Furthermore, Cytoskeleton, Inc. guarantees that the cost of each kit is substantially less than the sum of the component parts purchased separately.  Cytoskeleton, Inc. provides Biochem Kits for actin, tubulin, G-protein activation, G-protein signal transduction and molecular motor research.

If you are new to the field of cytoskeletal research, the Biochem Kits will prove to be an invaluable introductory aid, providing many useful technical tips. For example, if you are new to the field of microtubule research, you may not know that tubulin requires GTP, Mg2+ and >50 mM PIPES for stability and that taxol (a microtubule stabilizing drug) added too quickly will cause aberrant tubulin arrangements to form. If any of these points are overlooked, the results could lead to inappropriate experimental interpretation.Biochem kits are designed to overcome this bottleneck in research.

Question 2: How can I use the Biochem Kits to investigate whether my protein interacts with the cytoskeleton or its regulatory components?

Answer 2:Cytoskeleton, Inc. has a variety of kits that can help the researcher examine how recombinant proteins, drugs or even cell lysates either interact and/or affect the function of cytoskeletal proteins including monomers and polymers of actin and tubulin. 

Some examples of kits specifically designed for this purpose include the actin binding protein assay Biochem Kit (Cat. # BK001 or BK013) and the tubulin binding protein assay Biochem Kit (Cat.  # BK029). 

Actin binding protein assay Biochem kit (Cat.  # BK001 or BK013): This kit provides clear instructions and all the reagents and controls necessary to determine the ability of a given protein to bind to actin monomers versus actin filaments.  F-actin binding can be measured by using a spin down assay where centrifugation is used to separate F-actin from G-actin by differential sedimentation.  This kit can be used to determine (i) whether a test protein binds F-actin or affects the equilibrium between G-actin and F-actin, (ii) whether a test protein has G-actin sequestering or F-actin polymerization enhancing activity or (iii) whether a test protein of interest can bundle F-actin.  We suggest coupling this assay with the Actin polymerization assay Biochem kit in order to obtain a more complete profile of your potential actin binding protein.

Tubulin binding protein assay Biochem Kit(Cat. # BK029):This kit provides clear instructions and all the reagents and controls necessary to determine the ability of a given protein to bind to tubulin monomers versus tubulin polymers (microtubules).  Tubulin binding can be measured by using a spin down assay where centrifugation is used to separate microtubules from tubulin monomers by differential sedimentation.  This kit can be used to: (i) identify novel microtubule associated proteins (MAPs), (ii) confirm in vivodata suggesting a given protein is a MAP, (iii) characterize MAPs, (iv) identify/characterize MAP regulating proteins or (v) identify/characterize compounds that inhibit MAP binding to microtubules.  We suggest coupling this assay with the tubulin polymerization assay Biochem Kit (Cat. # BK006P or BK011P) in order to obtain a more complete profile of your potential tubulin binding protein. 

 

Actin polymerization assay Biochem kit (Cat. # BK003): This kit uses modified actin to follow polymerization kinetics. There are many types of actin binding proteins, including nucleating, capping, severing, side-binding and monomer sequestering actin binding proteins. All of these can be characterized by their effect on one or more of the three stages of actin polymerization: nucleation, growth and steady-state equilibrium.  A compound’s effects on depolymerization can also be evaluated. Cytoskeleton has adapted this powerful assay into a user friendly Biochem kit format.

Tubulin polymerization assay Biochem kit (Cat. # BK006P or BK001P): We offer both absorbance-based and fluorescence-based tubulin polymerization kits to follow polymerization kinetics.  The absorbance-based kit (Cat. # BK006P) takes advantage of the fact that light is scattered by microtubules to an extent that is proportional to the concentration of microtubule polymer.  The fluorescence-based kit (Cat. # BK011P) measures polymerization as a function of fluorescence enhancement following the incorporation of a fluorescent reporter into microtubules as polymerization occurs.  There are many proteins and drugs that either enhance or inhibit polymerization or depolymerization.  These compounds can be characterized by their effect on one or more of the three stages of tubulin polymerization: nucleation, growth, and steady-state equilibrium.  A compound’s effects on depolymerization can also be evaluated. Cytoskeleton has adapted these powerful assays into user friendly Biochem Kit formats.

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