Kits & Assays

Cytoskeleton's Biochem Kits™ are comprehensive kits for assaying different aspects of signal transduction processes and cytoskeletal biochemistry.  These kits help scientists produce publication quality data in a short period of time (Click citation tab above for examples).  These kits come with all the reagents needed for the assay as well as detailed instruction on how to use them, so you will be ready to perform experiments as soon as the kits arrives!

 

For more information about our Biochem Kits please click the About tab above.

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About Cytoskeleton's Biochem™ kits

Biochem™ kits are an extremely quick way to become familiar with some of the most useful in vitro and in vivo assays in signal transduction and cytoskeletal research. Each kit is carefully quality controlled and very user friendly and will therefore save time and money even for experienced cytoskeletal researchers. Cytoskeleton provides Biochem kits for actin, tubulin, intermediate filament, small G-protein, signal transduction and molecular motor research.

If you are new to the field of cytoskeletal research, the Biochem™ kits will prove to be an invaluable introductory aid, providing many useful technical tips. For example, if you are new to the field of microtubule research, you may not know that tubulin requires GTP, Mg2+ and >50 mM PIPES for stability and that taxol (a microtubule stabilizing drug) added too quickly will cause aberrant tubulin arrangements to form. If either of these points are overlooked, the results could lead to inappropriate experimental interpretation.Biochem™ kits are designed to overcome this bottleneck in research.

As an example: If you wanted to know whether a specific protein or compound interacts with actin filaments and/or monomer, you could order one of several Biochem kits specializing in actin research:

1) Actin binding protein assay Biochem™ kit (Cat. # BK001 or BK013): This kit provides clear instructions and all the reagents and controls necessary to determine the ability of a given protein to bind to actin filaments. It should be noted that in some cases actin binding proteins have a low affinity for actin filaments. Therefore, it is wise to couple this assay with the Actin polymerization assay Biochem™ kit in order to obtain a more complete profile of your potential actin binding protein.

2) Actin polymerization assay Biochem™ kit (Cat. # BK003): This kit uses modified actin to follow polymerization kinetics. There are many types of actin binding proteins, including nucleating, capping, severing, side-binding and monomer sequestering actin binding proteins. All of these can be characterized by their effect on actin polymerization. Cytoskeleton has adapted this powerful assay into a user friendly Biochem™ kit format.

Biochem™ kits are also a great way to optimize your research time and money. When you buy a Biochem™ kit, you obtain all the necessary proteins, buffers, controls and instructions that you require to perform your research with the highest degree of confidence. Furthermore, Cytoskeleton Inc guarantees that the cost of each kit is substantially less than the sum of the component parts purchased separately.

Many publications cite the use of Cytoskeleton's kits in the Materials and Methods section of papers. Usually the citation is associated with a particular result in the form of a graph or image that helps the authors present their findings. This indicates the utility of the Kits to produce publication quality data in a short timeframe thus helping improve the productivity of your efforts. Example citations for the BK011P kit is shown, but more citations are available on individual product pages.



AuthorTitleJournalYearArticle Link
Aggarwal, Ranjana et al.Design, Synthesis, and In Vitro Cytotoxic Studies of Some Novel Arylidene-Hydrazinyl-Thiazoles as Anticancer and Apoptosis-Inducing AgentsACS Omega2024
Zou, Zheng et al.Pocket-based lead optimization strategy for the design and synthesis of tubulin polymerization inhibitorsResults in Chemistry2024
Pospíšilová, Jana et al.Atropisomeric 1-phenylbenzimidazoles affecting microtubule organization: influence of axial chiralityOrganic & Biomolecular Chemistry2024
Abdelmegeed, Heba et al.Exploring the antitumor potential of novel quinoline derivatives via tubulin polymerization inhibition in breast cancer; design, synthesis and molecular dockingRSC Advances2024
Řehulka, Jiří et al.Click estradiol dimers with novel aromatic bridging units: synthesis and anticancer evaluationJournal of Enzyme Inhibition and Medicinal Chemistry2024
Wang, Chao et al.Design, synthesis, and bioevaluation of 1h-pyrrolo[3,2-c]pyridine derivatives as colchicine-binding site inhibitors with potent anticancer activitiesJournal of Enzyme Inhibition and Medicinal Chemistry2024
Ezelarab, Hend A.A. et al.New antiproliferative 3-substituted oxindoles inhibiting EGFR/VEGFR-2 and tubulin polymerizationMolecular Diversity2024
El-Zoghbi, Mona S. et al.Design, Synthesis and Mechanistic Study of New Dual Targeting HDAC/Tubulin InhibitorsFuture Medicinal Chemistry2024
Shahjahan, S. et al.Oxindoline Containing Thiazolidine-4-one Tethered Triazoles Act as Antimitotic Agents by Targeting Microtubule DynamicsChemistrySelect2024
Wang, Chao et al.3-aryl-4-(3,4,5-trimethoxyphenyl)pyridines inhibit tubulin polymerisation and act as anticancer agentsJournal of Enzyme Inhibition and Medicinal Chemistry2024
Xiu, Yutao et al.Design, synthesis, and bioevaluation of diarylpyrimidine derivatives as novel microtubule destabilizersFrontiers in Chemistry2024
El-Sayed, Ashraf S.A. et al.Bioprocessing of Epothilone B from Aspergillus fumigatus under solid state fermentation: Antiproliferative activity, tubulin polymerization and cell cycle analysisBMC Microbiology2024
Sun, Ning et al.The study of hon***** as a natural product-based antimicrobial agent and its potential interaction with FtsZ proteinFrontiers in Microbiology2024
Patrón, Lilian A. et al.Novel Brain-Penetrant, Small-Molecule Tubulin Destabilizers for the Treatment of GlioblastomaBiomedicines2024
Bouzriba, Chahrazed et al.Design, synthesis and biological evaluation of new 2,6-difluorinated phenyl 4-(2-oxoimidazolidin-1-yl)benzenesulfonates as new antimicrotubule agentsBioorganic Chemistry2024
Lin, H.-Y ; et al.From Sea to Science: Coral Aquaculture for Sustainable Anticancer Drug DevelopmentMarine Drugs 2024
Patterson, Jesse C. et al.Plk1 Inhibitors and Abiraterone Synergistically Disrupt Mitosis and Kill Cancer Cells of Disparate Origin Independently of Androgen Receptor SignalingCancer Research2023
Payton, Marc et al.Small-molecule inhibition of kinesin KIF18A reveals a mitotic vulnerability enriched in chromosomally unstable cancersNature Cancer2023
Grzywa, Renata et al.Isothiocyanates as Tubulin Polymerization Inhibitors—Synthesis and Structure–Activity Relationship StudiesInternational Journal of Molecular Sciences2023
Hou, Zhenyan et al.S-72, a Novel Orally Available Tubulin Inhibitor, Overcomes Pac******* Resistance via Inactivation of the STING Pathway in Breast CancerPharmaceuticals2023
Liang, Baoxia et al.Novel Indole-Containing Hybrids Derived from Millepachine: Synthesis, Biological Evaluation and Antitumor Mechanism StudyMolecules2023
Wang, Chao et al.Design, synthesis, and biological evaluation of 4-aryl-9H-carbazoles as tubulin polymerization inhibitors with potent anticancer activitiesArabian Journal of Chemistry2023
Horgan, Mark James et al.Identification of Novel β-Tubulin Inhibitors Using a Combined In Silico/In Vitro ApproachJournal of Chemical Information and Modeling2023
Li, Yuan-Yuan et al.Tetrabromobisphenol A-bis(2,3-dibromopropyl ether) impairs Postnatal Testis Development in Mice: The Microtubule Cytoskeleton as a Sensitive TargetEnvironment & Health2023
Dong, Haiyang et al.Design, synthesis and biological evaluation of tetrahydroquinoxaline sulfonamide derivatives as colch***** binding site inhibitorsRSC Advances2023
Morishita, Jun et al.Identification of a small RhoA GTPase inhibitor effective in fission yeast and human cellsOpen Biology2023
Yao, Yongfang et al.Angiogenesis and anti-leukaemia activity of novel indole derivatives as potent colch***** binding site inhibitorsJournal of Enzyme Inhibition and Medicinal Chemistry2022
Wang, Zhan et al.Design, synthesis and biological evaluation of colch***** glycoconjugates as tubulin polymerization inhibitorsBioorganic and Medicinal Chemistry2022
Ma, Juan et al.Discovery of Novel 3,4-Dihydro-2(1H)-Quinolinone Sulfonamide Derivatives as New Tubulin Polymerization Inhibitors with Anti-Cancer ActivityMolecules2022
Liu, Chao et al.Synthesis and biological evaluation of BU-4664L derivatives as potential anticancer agentsBioorganic and Medicinal Chemistry Letters2022
Kumar N, Manoj et al.Benzothiazole-[1,2,3]triazolo[5,1-a]isoindoles: Synthesis, anticancer activity, bioavailability and in silico studies against Gama-Tubulin proteinJournal of Molecular Structure2022
Woo, Jung AA et al.β-arrestin1 promotes tauopathy by transducing GPCR signaling, disrupting microtubules and autophagyLife science alliance2021
Yang, Mei et al.C118P, a novel microtubule inhibitor with anti-angiogenic and vascular disrupting activities, exerts anti-tumor effects against hepatocellular carcinomaBiochemical Pharmacology2021
Sun, Chiao Yin et al.LMBD1 protein participates in cell mitosis by regulating microtubule assemblyBiochemical Journal2021
Liang, Dong et al.Identification of anthelmintic parbendazole as a therapeutic molecule for HNSCC through connectivity map-based drug repositioningActa Pharmaceutica Sinica B2021
Rahimzadeh Oskuei, Sara et al.Design, synthesis and biological evaluation of novel imidazole-chalcone derivatives as potential anticancer agents and tubulin polymerization inhibitorsBioorganic Chemistry2021
Wang, Chao et al.Design, synthesis and biological evaluation of 1-Aryl-5-(4-arylpiperazine-1-carbonyl)-1H-tetrazols as novel microtubule destabilizersJournal of Enzyme Inhibition and Medicinal Chemistry2021
Patel, Onisha et al.Structural basis for small molecule targeting of Doublecortin Like Kinase 1 with DCLK1-IN-1Communications Biology2021
Fareed, Momen R. et al.New multi-targeted antiproliferative agents: Design and synthesis of ic261-based oxindoles as potential tubulin, ck1 and egfr inhibitorsPharmaceuticals2021
Zhernov, Ilia et al.Intrinsically Disordered Domain of Kinesin-3 Kif14 Enables Unique Functional DiversityCurrent Biology2020
Baker, Stacey J. et al.A Contaminant Impurity, Not Rigosertib, Is a Tubulin Binding AgentMolecular Cell2020
Skidmore, Lillian et al.ARX788, a site-specific anti-HER2 antibody–drug conjugate, demonstrates potent and selective activity in HER2-low and T-DM1–resistant breast and gastric cancersMolecular Cancer Therapeutics2020
Wang, Yanming et al.Antibody-drug conjugate using ionized CYS-linker-mmae as the potent payload shows optimal therapeutic safetyCancers2020
Morita, Ken et al.Allosteric Activators of Protein Phosphatase 2A Display Broad Antitumor Activity Mediated by Dephosphorylation of MYBL2Cell2020
Dillon, Gregory M. et al.Acute inhibition of the CNS-specific kinase TTBK1 significantly lowers tau phosphorylation at several disease relevant sitesPLoS ONE2020
Ullah, Imran et al.An Antiparasitic Compound from the Medicines for Malaria Venture Pathogen Box Promotes Leishmania Tubulin PolymerizationACS Infectious Diseases2020
Song, Ming Yu et al.Exploring diverse-ring analogues on combretastatin a4 (Ca-4) olefin as microtubule-targeting agentsInternational Journal of Molecular Sciences2020
Chen, Shih Yin et al.Exosomal 2′,3′-CNP from mesenchymal stem cells promotes hippocampus CA1 neurogenesis/neuritogenesis and contributes to rescue of cognition/learning deficiencies of damaged brainStem Cells Translational Medicine2020
Zdioruk, Mykola et al.A new inhibitor of tubulin polymerization kills multiple cancer cell types and reveals p21-mediated mechanism determining cell death after mitotic catastropheCancers2020
Mirzaei, Salimeh et al.Synthesis, structure-activity relationship and molecular docking studies of novel quinoline-chalcone hybrids as potential anticancer agents and tubulin inhibitorsJournal of Molecular Structure2020
Palumbo, Valeria et al.Drosophila Morgana is an Hsp90-interacting protein with a direct role in microtubule polymerizationJournal of Cell Science2020
Barnes, Natalie G. et al.A 2-step synthesis of Combretastatin A-4 and derivatives as potent tubulin assembly inhibitorsBioorganic and Medicinal Chemistry2020
Mao, Bai Ping et al.CaMSAP2 is a microtubule minus-end targeting protein that regulates BTB dynamics through cytoskeletal organizationEndocrinology2019
Behbahani, Fatemeh Shaebani et al.Synthesis and biological evaluation of novel benzo[c]acridine-diones as potential anticancer agents and tubulin polymerization inhibitorsArchiv der Pharmazie2019
Cui, Ying Jie et al.Synthesis of novel pyrazole derivatives and their tumor cell growth inhibitory activityMolecules2019
Xia, Xiaoyu et al.Leukemia Cell Cycle Chemical Profiling Identifies the G2-Phase Leukemia Specific Inhibitor Leusin-1ACS Chemical Biology2019
Wang, Yanming et al.Novel silyl ether-based acid-cleavable antibody-MMAE conjugates with appropriate stability and efficacyCancers2019
Shaik, Thoukhir B. et al.Evaluation of Anticancer and Anti-Mitotic Properties of Quinazoline and Quinazolino-Benzothiadiazine DerivativesAnti-Cancer Agents in Medicinal Chemistry2019
Nixon, Gemma L. et al.Repurposing and reformulation of the antiparasitic agent flubendazole for treatment of cryptococcal meningoencephalitis, a neglected fungal diseaseAntimicrobial Agents and Chemotherapy2018
Qi, Jianguo et al.Synthesis and biological evaluation of N-substituted 3-oxo-1,2,3,4-tetrahydro-quinoxaline-6-carboxylic acid derivatives as tubulin polymerization inhibitorsEuropean Journal of Medicinal Chemistry2018
Simpkins, Scott W. et al.Predicting bioprocess targets of chemical compounds through integration of chemical-genetic and genetic interactionsPLoS Computational Biology2018
Sato-Kaneko, Fumi et al.Discovery of a Novel Microtubule Targeting Agent as an Adjuvant for Cancer ImmunotherapyBioMed Research International2018
Dilworth, David et al.The prolyl isomerase FKBP25 regulates microtubule polymerization impacting cell cycle progression and genomic stabilityNucleic Acids Research2018
Tian, Zhenhua et al.Biological activity and interaction mechanism of the diketopiperazine derivatives as tubulin polymerization inhibitorsRSC Advances2018
Huan, L C et al.Exploration of Some Thiazolidine-2, 4-dione and 2-Oxoindoline Derivatives Incorporating 3, 4, 5-Trimethoxybenzyl Moiety as Novel Anticancer AgentsLetters in Drug …2018
Lukinavičius, Gražvydas et al.Fluorescent dyes and probes for super-resolution microscopy of microtubules and tracheoles in living cells and tissuesChemical Science2018
Chen, Minfeng et al.Pericyte-targeting prodrug overcomes tumor resistance to vascular disrupting agentsJournal of Clinical Investigation2017
Wieczorek, Anna et al.Synthesis and evaluation of biological properties of ferrocenyl-podophyllotoxin conjugatesDalton Transactions2017
Zollo, Massimo et al.PRUNE is crucial for normal brain development and mutated in microcephaly with neurodevelopmental impairmentBrain2017
Kowalczyk, Karolina et al.Colch***** metallocenyl bioconjugates showing high antiproliferative activities against cancer cell linesDalton Transactions2017
Tantak, Mukund P. et al.Design and synthesis of bis(indolyl)ketohydrazide-hydrazones: Identification of potent and selective novel tubulin inhibitorsEuropean Journal of Medicinal Chemistry2017
Sun, Maolin et al.Synthesis and bioevaluation of N,4-diaryl-1,3-thiazole-2-amines as tubulin inhibitors with potent antiproliferative activityPLoS ONE2017
Wu, Yue et al.Design and synthesis of 5-aryl-4-(4-arylpiperazine-1-carbonyl)-2H-1,2,3-triazole derivatives as colch***** binding site inhibitorsScientific Reports2017
Pearson, Brandon L. et al.Identification of chemicals that mimic transcriptional changes associated with autism, brain aging and neurodegenerationNature Communications2016
Fukuda, Yasunori et al.Tubulin is a molecular target of the Wnt-activating chemical probeBMC Biochemistry2016
Thapa, Pritam et al.Far-Red Light-Activatable Prodrug of Pac******* for the Combined Effects of Photodynamic Therapy and Site-Specific Pac******* ChemotherapyJournal of Medicinal Chemistry2016
Yoshitake, Jun et al.Modification of tau by 8-nitroguanosine 3′,5′-cyclic monophosphate (8-nitro-cGMP): Effects of nitric oxide-linked chemical modification on tau aggregationJournal of Biological Chemistry2016
Hasanpourghadi, Mohadeseh et al.Targeting of tubulin polymerization and induction of mitotic blockage by Methyl 2-(5-fluoro-2-hydroxyphenyl)-1H-benzo[d]imidazole-5-carboxylate (MBIC) in human cervical cancer HeLa cellJournal of Experimental and Clinical Cancer Research2016
Cai, De et al.YSL-12, a novel microtubule-destabilizing agent, exerts potent anti-tumor activity against colon cancer in vitro and in vivoCancer Chemotherapy and Pharmacology2016
Remers, William A. et al.Synthesis and Antitumor Activity of Heterocycles Related to CarbendazimJournal of Heterocyclic Chemistry2015
Fawzy, Iten M. et al.Newly Designed and Synthesized Curcumin Analogs with in vitro Cytotoxicity and Tubulin Polymerization ActivityChemical biology & drug design2015
Seashore-Ludlow, Brinton et al.Harnessing connectivity in a large-scale small-molecule sensitivity datasetCancer Discovery2015
Yan, Jun et al.A novel synthetic compound exerts effective anti-tumour activity in vivo via the inhibition of tubulin polymerisation in A549 cellsBiochemical Pharmacology2015
Sheldon, Jonathon E. et al.Photoswitchable anticancer activity via trans-cis isomerization of a combretastatin A-4 analogOrganic and Biomolecular Chemistry2015
Calles, Antonio et al.Tivantinib (ARQ 197) efficacy is independent of MET inhibition in non-small-cell lung cancer cell linesMolecular Oncology2015
Choi, Bo Hwa et al.Suprafenacine, an Indazole-Hydrazide Agent, Targets Cancer Cells Through Microtubule DestabilizationPLOS ONE2014
Shigehiro, Tsukasa et al.Efficient Drug Delivery of Pac******* Glycoside: A Novel Solubility Gradient Encapsulation into Liposomes Coupled with Immunoliposomes PreparationPLOS ONE2014
Senese, S. et al.Chemical dissection of the cell cycle: Probes for cell biology and anti-cancer drug developmentCell Death and Disease2014
Bio, Moses et al.Far-red light activatable, multifunctional prodrug for fluorescence optical imaging and combinational treatmentJournal of Medicinal Chemistry2014
Mäki-Jouppila, Jenni H.E. et al.Centmitor-1, a novel acridinyl-acetohydrazide, possesses similar molecular interaction field and antimitotic cellular phenotype as rigosertib, on 01910.NaMolecular Cancer Therapeutics2014
Bio, Moses et al.Site-specific and far-red-light-activatable prodrug of combretastatin A-4 using photo-unclick chemistryJournal of Medicinal Chemistry2013
Sidhaye, Venkataramana K. et al.A Novel Role for Aquaporin-5 in Enhancing Microtubule Organization and StabilityPLOS ONE2012
Zach, Frank et al.The retinitis pigmentosa 28 protein FAM161A is a novel ciliary protein involved in intermolecular protein interaction and microtubule associationHuman molecular genetics2012
Fitzgerald, Daniel P. et al.TPI-287, a new taxane family member, reduces the brain metastatic colonization of breast cancer cellsMolecular Cancer Therapeutics2012
Nguyen, Tam Luong et al.Evading Pgp activity in drug-resistant cancer cells: A structural and functional study of antitubulin furan metotica compoundsMolecular Cancer Therapeutics2012
Dyrager, Christine et al.Inhibitors and promoters of tubulin polymerization: synthesis and biological evaluation of chalcones and related dienones as potential anticancer agentsBioorganic & medicinal chemistry2011
Hwang, Ji Hwan et al.Induction of tubulin polymerization and apoptosis in malignant mesothelioma cells by a new compound JBIR-23Cancer letters2011
Kim, Insook et al.Zinc stimulates tau S214 phosphorylation by the activation of Raf/mitogen-activated protein kinase-kinase/extracellular signal-regulated kinase pathwayNeuroReport2011
Kawaratani, Yasuyuki et al.New microtubule polymerization inhibitors comprising a nitrooxymethylphenyl groupBioorganic and Medicinal Chemistry2011
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Question 1:  How can Cytoskeleton’s Biochem Kits help me in my research?

Question 2:  How can I use the Biochem Kits to investigate whether my protein interacts with the cytoskeleton or its regulatory components?

 


Question 1: How can Cytoskeleton’s Biochem Kits help me in my research?

Answer 1:Cytoskeleton's Biochem Kits are an extremely quick way to become familiar with some of the most useful in vitro and in vivo assays for assaying different aspects of cytoskeletal biochemistry and signal transduction. These kits come with all the reagents needed for your assay as well as detailed instruction on how to use them, so you will be ready to do your assays as soon as you have the kits!  Each kit is carefully quality controlled and very user friendly and will therefore save time and money even for experienced cytoskeletal researchers.  Furthermore, Cytoskeleton, Inc. guarantees that the cost of each kit is substantially less than the sum of the component parts purchased separately.  Cytoskeleton, Inc. provides Biochem Kits for actin, tubulin, G-protein activation, G-protein signal transduction and molecular motor research.

If you are new to the field of cytoskeletal research, the Biochem Kits will prove to be an invaluable introductory aid, providing many useful technical tips. For example, if you are new to the field of microtubule research, you may not know that tubulin requires GTP, Mg2+ and >50 mM PIPES for stability and that taxol (a microtubule stabilizing drug) added too quickly will cause aberrant tubulin arrangements to form. If any of these points are overlooked, the results could lead to inappropriate experimental interpretation.Biochem kits are designed to overcome this bottleneck in research.

Question 2: How can I use the Biochem Kits to investigate whether my protein interacts with the cytoskeleton or its regulatory components?

Answer 2:Cytoskeleton, Inc. has a variety of kits that can help the researcher examine how recombinant proteins, drugs or even cell lysates either interact and/or affect the function of cytoskeletal proteins including monomers and polymers of actin and tubulin. 

Some examples of kits specifically designed for this purpose include the actin binding protein assay Biochem Kit (Cat. # BK001 or BK013) and the tubulin binding protein assay Biochem Kit (Cat.  # BK029). 

Actin binding protein assay Biochem kit (Cat.  # BK001 or BK013): This kit provides clear instructions and all the reagents and controls necessary to determine the ability of a given protein to bind to actin monomers versus actin filaments.  F-actin binding can be measured by using a spin down assay where centrifugation is used to separate F-actin from G-actin by differential sedimentation.  This kit can be used to determine (i) whether a test protein binds F-actin or affects the equilibrium between G-actin and F-actin, (ii) whether a test protein has G-actin sequestering or F-actin polymerization enhancing activity or (iii) whether a test protein of interest can bundle F-actin.  We suggest coupling this assay with the Actin polymerization assay Biochem kit in order to obtain a more complete profile of your potential actin binding protein.

Tubulin binding protein assay Biochem Kit(Cat. # BK029):This kit provides clear instructions and all the reagents and controls necessary to determine the ability of a given protein to bind to tubulin monomers versus tubulin polymers (microtubules).  Tubulin binding can be measured by using a spin down assay where centrifugation is used to separate microtubules from tubulin monomers by differential sedimentation.  This kit can be used to: (i) identify novel microtubule associated proteins (MAPs), (ii) confirm in vivodata suggesting a given protein is a MAP, (iii) characterize MAPs, (iv) identify/characterize MAP regulating proteins or (v) identify/characterize compounds that inhibit MAP binding to microtubules.  We suggest coupling this assay with the tubulin polymerization assay Biochem Kit (Cat. # BK006P or BK011P) in order to obtain a more complete profile of your potential tubulin binding protein. 

 

Actin polymerization assay Biochem kit (Cat. # BK003): This kit uses modified actin to follow polymerization kinetics. There are many types of actin binding proteins, including nucleating, capping, severing, side-binding and monomer sequestering actin binding proteins. All of these can be characterized by their effect on one or more of the three stages of actin polymerization: nucleation, growth and steady-state equilibrium.  A compound’s effects on depolymerization can also be evaluated. Cytoskeleton has adapted this powerful assay into a user friendly Biochem kit format.

Tubulin polymerization assay Biochem kit (Cat. # BK006P or BK001P): We offer both absorbance-based and fluorescence-based tubulin polymerization kits to follow polymerization kinetics.  The absorbance-based kit (Cat. # BK006P) takes advantage of the fact that light is scattered by microtubules to an extent that is proportional to the concentration of microtubule polymer.  The fluorescence-based kit (Cat. # BK011P) measures polymerization as a function of fluorescence enhancement following the incorporation of a fluorescent reporter into microtubules as polymerization occurs.  There are many proteins and drugs that either enhance or inhibit polymerization or depolymerization.  These compounds can be characterized by their effect on one or more of the three stages of tubulin polymerization: nucleation, growth, and steady-state equilibrium.  A compound’s effects on depolymerization can also be evaluated. Cytoskeleton has adapted these powerful assays into user friendly Biochem Kit formats.

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