Cytoskeleton's Biochem Kits™ are comprehensive kits for assaying different aspects of signal transduction processes and cytoskeletal biochemistry. These kits help scientists produce publication quality data in a short period of time (Click citation tab above for examples). These kits come with all the reagents needed for the assay as well as detailed instruction on how to use them, so you will be ready to perform experiments as soon as the kits arrives!
For more information about our Biochem Kits please click the About tab above.
About Cytoskeleton's Biochem™ kits
Biochem™ kits are an extremely quick way to become familiar with some of the most useful in vitro and in vivo assays in signal transduction and cytoskeletal research. Each kit is carefully quality controlled and very user friendly and will therefore save time and money even for experienced cytoskeletal researchers. Cytoskeleton provides Biochem kits for actin, tubulin, intermediate filament, small G-protein, signal transduction and molecular motor research.
If you are new to the field of cytoskeletal research, the Biochem™ kits will prove to be an invaluable introductory aid, providing many useful technical tips. For example, if you are new to the field of microtubule research, you may not know that tubulin requires GTP, Mg2+ and >50 mM PIPES for stability and that taxol (a microtubule stabilizing drug) added too quickly will cause aberrant tubulin arrangements to form. If either of these points are overlooked, the results could lead to inappropriate experimental interpretation.Biochem™ kits are designed to overcome this bottleneck in research.
As an example: If you wanted to know whether a specific protein or compound interacts with actin filaments and/or monomer, you could order one of several Biochem kits specializing in actin research:
1) Actin binding protein assay Biochem™ kit (Cat. # BK001 or BK013): This kit provides clear instructions and all the reagents and controls necessary to determine the ability of a given protein to bind to actin filaments. It should be noted that in some cases actin binding proteins have a low affinity for actin filaments. Therefore, it is wise to couple this assay with the Actin polymerization assay Biochem™ kit in order to obtain a more complete profile of your potential actin binding protein.
2) Actin polymerization assay Biochem™ kit (Cat. # BK003): This kit uses modified actin to follow polymerization kinetics. There are many types of actin binding proteins, including nucleating, capping, severing, side-binding and monomer sequestering actin binding proteins. All of these can be characterized by their effect on actin polymerization. Cytoskeleton has adapted this powerful assay into a user friendly Biochem™ kit format.
Biochem™ kits are also a great way to optimize your research time and money. When you buy a Biochem™ kit, you obtain all the necessary proteins, buffers, controls and instructions that you require to perform your research with the highest degree of confidence. Furthermore, Cytoskeleton Inc guarantees that the cost of each kit is substantially less than the sum of the component parts purchased separately.
Many publications cite the use of Cytoskeleton's kits in the Materials and Methods section of papers. Usually the citation is associated with a particular result in the form of a graph or image that helps the authors present their findings. This indicates the utility of the Kits to produce publication quality data in a short timeframe thus helping improve the productivity of your efforts. Example citations for the BK011P kit is shown, but more citations are available on individual product pages.
|Kumar N, Manoj et al.||Benzothiazole-[1,2,3]triazolo[5,1-a]isoindoles: Synthesis, anticancer activity, bioavailability and in silico studies against Gama-Tubulin protein||Journal of Molecular Structure||2022||ISSN 0022-2860|
|Yao, Yongfang et al.||Angiogenesis and anti-leukaemia activity of novel indole derivatives as potent colchicine binding site inhibitors||Journal of Enzyme Inhibition and Medicinal Chemistry||2022||ISSN 1475-6374|
|Wang, Zhan et al.||Design, synthesis and biological evaluation of colchicine glycoconjugates as tubulin polymerization inhibitors||Bioorganic and Medicinal Chemistry||2022||ISSN 1464-3391|
|Ma, Juan et al.||Discovery of Novel 3,4-Dihydro-2(1H)-Quinolinone Sulfonamide Derivatives as New Tubulin Polymerization Inhibitors with Anti-Cancer Activity||Molecules||2022||ISSN 1420-3049|
|Würtz, Martin et al.||Modular assembly of the principal microtubule nucleator γ-TuRC||Nature Communications||2022||ISSN 2041-1723|
|Liu, Chao et al.||Synthesis and biological evaluation of BU-4664L derivatives as potential anticancer agents||Bioorganic and Medicinal Chemistry Letters||2022||ISSN 1464-3405|
|Wang, Guangcheng et al.||Design, synthesis and biological evaluation of novel 2-phenyl-4,5,6,7-tetrahydro-1H-indole derivatives as potential anticancer agents and tubulin polymerization inhibitors||Arabian Journal of Chemistry||2022||ISSN 1878-5352|
|Patel, Onisha et al.||Structural basis for small molecule targeting of Doublecortin Like Kinase 1 with DCLK1-IN-1||Communications Biology||2021||ISSN 2399-3642|
|Fareed, Momen R. et al.||New multi-targeted antiproliferative agents: Design and synthesis of ic261-based oxindoles as potential tubulin, ck1 and egfr inhibitors||Pharmaceuticals||2021||ISSN 1424-8247|
|Rahimzadeh Oskuei, Sara et al.||Design, synthesis and biological evaluation of novel imidazole-chalcone derivatives as potential anticancer agents and tubulin polymerization inhibitors||Bioorganic Chemistry||2021||ISSN 1090-2120|
|Liang, Dong et al.||Identification of anthelmintic parbendazole as a therapeutic molecule for HNSCC through connectivity map-based drug repositioning||Acta Pharmaceutica Sinica B||2021||ISSN 2211-3843|
|Sun, Chiao Yin et al.||LMBD1 protein participates in cell mitosis by regulating microtubule assembly||Biochemical Journal||2021||ISSN 1470-8728|
|Wang, Chao et al.||Design, synthesis and biological evaluation of 1-Aryl-5-(4-arylpiperazine-1-carbonyl)-1H-tetrazols as novel microtubule destabilizers||Journal of Enzyme Inhibition and Medicinal Chemistry||2021||ISSN 1475-6374|
|Würtz, Martin et al.||Reconstitution of the recombinant human γ-tubulin ring complex||Open Biology||2021||ISSN 2046-2441|
|Grohmann, Christoph et al.||Preclinical small molecule WEHI-7326 overcomes drug resistance and elicits response in patient-derived xenograft models of human treatment-refractory tumors||Cell Death and Disease||2021||ISSN 2041-4889|
|Yang, Mei et al.||C118P, a novel microtubule inhibitor with anti-angiogenic and vascular disrupting activities, exerts anti-tumor effects against hepatocellular carcinoma||Biochemical Pharmacology||2021|
|Lead optimization of novel quinolone chalcone compounds by a structure-activity relationship (SAR) study to increase efficacy and metabolic stability||2021||Article Link|
|Shawky, Ahmed M. et al.||Novel pyrrolizines bearing 3,4,5-trimethoxyphenyl moiety: design, synthesis, molecular docking, and biological evaluation as potential multi-target cytotoxic agents||Journal of Enzyme Inhibition and Medicinal Chemistry||2021||ISSN 1475-6374|
|Woo, Jung AA et al.||β-arrestin1 promotes tauopathy by transducing GPCR signaling, disrupting microtubules and autophagy||Life science alliance||2021||ISSN 2575--1077|
|Liu, Qian et al.||Identification of a lathyrane-type diterpenoid EM-E-11-4 as a novel paclitaxel resistance reversing agent with multiple mechanisms of action||Aging||2020||ISSN 1945-4589|
|Abdel-Rahman, Somaya A. et al.||Cyclohepta[ b]thiophenes as Potential Antiproliferative Agents: Design, Synthesis, in Vitro, and in Vivo Anticancer Evaluation||ACS Pharmacology and Translational Science||2020||ISSN 2575-9108|
|Barnes, Natalie G. et al.||A 2-step synthesis of Combretastatin A-4 and derivatives as potent tubulin assembly inhibitors||Bioorganic and Medicinal Chemistry||2020||ISSN 1464-3391|
|Palumbo, Valeria et al.||Drosophila Morgana is an Hsp90-interacting protein with a direct role in microtubule polymerization||Journal of Cell Science||2020||ISSN 1477-9137|
|Mirzaei, Salimeh et al.||Synthesis, structure-activity relationship and molecular docking studies of novel quinoline-chalcone hybrids as potential anticancer agents and tubulin inhibitors||Journal of Molecular Structure||2020||ISSN 0022-2860|
|Chen, Shih Yin et al.||Exosomal 2′,3′-CNP from mesenchymal stem cells promotes hippocampus CA1 neurogenesis/neuritogenesis and contributes to rescue of cognition/learning deficiencies of damaged brain||Stem Cells Translational Medicine||2020||ISSN 2157-6580|
|Song, Ming Yu et al.||Exploring diverse-ring analogues on combretastatin a4 (Ca-4) olefin as microtubule-targeting agents||International Journal of Molecular Sciences||2020||ISSN 1422-0067|
|Ullah, Imran et al.||An Antiparasitic Compound from the Medicines for Malaria Venture Pathogen Box Promotes Leishmania Tubulin Polymerization||ACS Infectious Diseases||2020||ISSN 2373-8227|
|Tariq, Ammarah et al.||In vitro reconstitution of branching microtubule nucleation||eLife||2020||ISSN 2050-084X|
|Zhernov, Ilia et al.||Intrinsically Disordered Domain of Kinesin-3 Kif14 Enables Unique Functional Diversity||Current Biology||2020||ISSN 1879-0445|
|Zdioruk, Mykola et al.||A new inhibitor of tubulin polymerization kills multiple cancer cell types and reveals p21-mediated mechanism determining cell death after mitotic catastrophe||Cancers||2020||ISSN 2072-6694|
|Skidmore, Lillian et al.||ARX788, a site-specific anti-HER2 antibody–drug conjugate, demonstrates potent and selective activity in HER2-low and T-DM1–resistant breast and gastric cancers||Molecular Cancer Therapeutics||2020||ISSN 1538-8514|
|Wang, Yanming et al.||Antibody-drug conjugate using ionized CYS-linker-mmae as the potent payload shows optimal therapeutic safety||Cancers||2020||ISSN 2072-6694|
|Baker, Stacey J. et al.||A Contaminant Impurity, Not Rigosertib, Is a Tubulin Binding Agent||Molecular Cell||2020||ISSN 1097-4164|
|Morita, Ken et al.||Allosteric Activators of Protein Phosphatase 2A Display Broad Antitumor Activity Mediated by Dephosphorylation of MYBL2||Cell||2020||ISSN 1097-4172|
|Dillon, Gregory M. et al.||Acute inhibition of the CNS-specific kinase TTBK1 significantly lowers tau phosphorylation at several disease relevant sites||PLoS ONE||2020||ISSN 1932-6203|
|Zhai, Min'an et al.||3,5-Diaryl-1H-pyrazolo[3,4-b]pyridines as potent tubulin polymerization inhibitors: Rational design, synthesis and biological evaluation||European Journal of Medicinal Chemistry||2019||ISSN 1768-3254|
|Su, Wenhui et al.||Cdc42 is involved in NC1 peptide-regulated BTB dynamics through actin and microtubule cytoskeletal reorganization||FASEB journal : official publication of the Federation of American Societies for Experimental Biology||2019||ISSN 1530-6860|
|Wang, Yanming et al.||Novel silyl ether-based acid-cleavable antibody-MMAE conjugates with appropriate stability and efficacy||Cancers||2019||ISSN 2072-6694|
|Cui, Ying Jie et al.||Synthesis of novel pyrazole derivatives and their tumor cell growth inhibitory activity||Molecules||2019||ISSN 1420-3049|
|Shaik, Thoukhir B. et al.||Evaluation of Anticancer and Anti-Mitotic Properties of Quinazoline and Quinazolino-Benzothiadiazine Derivatives||Anti-Cancer Agents in Medicinal Chemistry||2019||ISSN 1871-5206|
|Sonawane, Vinay et al.||Cink4T, a quinazolinone-based dual inhibitor of Cdk4 and tubulin polymerization, identified via ligand-based virtual screening, for efficient anticancer therapy||European Journal of Medicinal Chemistry||2019||ISSN 1768-3254|
|Xia, Xiaoyu et al.||Leukemia Cell Cycle Chemical Profiling Identifies the G2-Phase Leukemia Specific Inhibitor Leusin-1||ACS Chemical Biology||2019||ISSN 1554-8937|
|Behbahani, Fatemeh Shaebani et al.||Synthesis and biological evaluation of novel benzo[c]acridine-diones as potential anticancer agents and tubulin polymerization inhibitors||Archiv der Pharmazie||2019||ISSN 1521-4184|
|Karimikia, Ehsan et al.||Colchicine-like β-acetamidoketones as inhibitors of microtubule polymerization: Design, synthesis and biological evaluation of in vitro anticancer activity||Iranian Journal of Basic Medical Sciences||2019||ISSN 2008-3874|
|Patterson, Jesse C. et al.||VISAGE Reveals a Targetable Mitotic Spindle Vulnerability in Cancer Cells||Cell Systems||2019||ISSN 2405-4720|
|Mao, Bai Ping et al.||CaMSAP2 is a microtubule minus-end targeting protein that regulates BTB dynamics through cytoskeletal organization||Endocrinology||2019||ISSN 1945-7170|
|Eberle-Singh, Jaime A. et al.||Effective delivery of a microtubule polymerization inhibitor synergizes with standard regimens in models of pancreatic ductal adenocarcinoma||Clinical Cancer Research||2019||ISSN 1557-3265|
|Lukinavičius, Gražvydas et al.||Fluorescent dyes and probes for super-resolution microscopy of microtubules and tracheoles in living cells and tissues||Chemical Science||2018||ISSN 2041-6539|
|Tian, Zhenhua et al.||Biological activity and interaction mechanism of the diketopiperazine derivatives as tubulin polymerization inhibitors||RSC Advances||2018||ISSN 2046-2069|
|Simpkins, Scott W. et al.||Predicting bioprocess targets of chemical compounds through integration of chemical-genetic and genetic interactions||PLoS Computational Biology||2018||ISSN 1553-7358|
|Nixon, Gemma L. et al.||Repurposing and reformulation of the antiparasitic agent flubendazole for treatment of cryptococcal meningoencephalitis, a neglected fungal disease||Antimicrobial Agents and Chemotherapy||2018||ISSN 1098-6596|
|Sato-Kaneko, Fumi et al.||Discovery of a Novel Microtubule Targeting Agent as an Adjuvant for Cancer Immunotherapy||BioMed Research International||2018||ISSN 2314-6141|
|Dilworth, David et al.||The prolyl isomerase FKBP25 regulates microtubule polymerization impacting cell cycle progression and genomic stability||Nucleic Acids Research||2018||ISSN 1362-4962|
|Huan, L C et al.||Exploration of Some Thiazolidine-2, 4-dione and 2-Oxoindoline Derivatives Incorporating 3, 4, 5-Trimethoxybenzyl Moiety as Novel Anticancer Agents||Letters in Drug …||2018||Article Link|
|Qi, Jianguo et al.||Synthesis and biological evaluation of N-substituted 3-oxo-1,2,3,4-tetrahydro-quinoxaline-6-carboxylic acid derivatives as tubulin polymerization inhibitors||European Journal of Medicinal Chemistry||2018||ISSN 1768-3254|
|Senese, Silvia et al.||Microtubins: A novel class of small synthetic microtubule targeting drugs that inhibit cancer cell proliferation||Oncotarget||2017||ISSN 1949-2553|
|Wieczorek, Anna et al.||Synthesis and evaluation of biological properties of ferrocenyl-podophyllotoxin conjugates||Dalton Transactions||2017||ISSN 1477-9234|
|Sun, Maolin et al.||Synthesis and bioevaluation of N,4-diaryl-1,3-thiazole-2-amines as tubulin inhibitors with potent antiproliferative activity||PLoS ONE||2017||ISSN 1932-6203|
|Zollo, Massimo et al.||PRUNE is crucial for normal brain development and mutated in microcephaly with neurodevelopmental impairment||Brain||2017||ISSN 1460-2156|
|Chen, Minfeng et al.||Pericyte-targeting prodrug overcomes tumor resistance to vascular disrupting agents||Journal of Clinical Investigation||2017||ISSN 1558-8238|
|Tantak, Mukund P. et al.||Design and synthesis of bis(indolyl)ketohydrazide-hydrazones: Identification of potent and selective novel tubulin inhibitors||European Journal of Medicinal Chemistry||2017||ISSN 1768-3254|
|Xu, Qile et al.||Design, synthesis and structure-Activity relationship of 3,6-diaryl-7H-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazines as novel tubulin inhibitors||Scientific Reports||2017||ISSN 2045-2322|
|Kowalczyk, Karolina et al.||Colchicine metallocenyl bioconjugates showing high antiproliferative activities against cancer cell lines||Dalton Transactions||2017||ISSN 1477-9234|
|Wu, Yue et al.||Design and synthesis of 5-aryl-4-(4-arylpiperazine-1-carbonyl)-2H-1,2,3-triazole derivatives as colchicine binding site inhibitors||Scientific Reports||2017||ISSN 2045-2322|
|Fu, Ying et al.||The contrasting catalytic efficiency and cancer cell antiproliferative activity of stereoselective organoruthenium transfer hydrogenation catalysts||Dalton Transactions||2016||ISSN 1477-9234|
|Cai, De et al.||YSL-12, a novel microtubule-destabilizing agent, exerts potent anti-tumor activity against colon cancer in vitro and in vivo||Cancer Chemotherapy and Pharmacology||2016||ISSN 1432-0843|
|Magalhaes, Luma G. et al.||Discovery of a series of acridinones as mechanism-based tubulin assembly inhibitors with anticancer activity||PLoS ONE||2016||ISSN 1932-6203|
|Hasanpourghadi, Mohadeseh et al.||Targeting of tubulin polymerization and induction of mitotic blockage by Methyl 2-(5-fluoro-2-hydroxyphenyl)-1H-benzo[d]imidazole-5-carboxylate (MBIC) in human cervical cancer HeLa cell||Journal of Experimental and Clinical Cancer Research||2016||ISSN 1756-9966|
|Pearson, Brandon L. et al.||Identification of chemicals that mimic transcriptional changes associated with autism, brain aging and neurodegeneration||Nature Communications||2016||ISSN 2041-1723|
|Yoshitake, Jun et al.||Modification of tau by 8-nitroguanosine 3′,5′-cyclic monophosphate (8-nitro-cGMP): Effects of nitric oxide-linked chemical modification on tau aggregation||Journal of Biological Chemistry||2016||ISSN 1083-351X|
|Thapa, Pritam et al.||Far-Red Light-Activatable Prodrug of Paclitaxel for the Combined Effects of Photodynamic Therapy and Site-Specific Paclitaxel Chemotherapy||Journal of Medicinal Chemistry||2016||ISSN 1520-4804|
|Fukuda, Yasunori et al.||Tubulin is a molecular target of the Wnt-activating chemical probe||BMC Biochemistry||2016||ISSN 1471-2091|
|Kamal, Ahmed et al.||Design, synthesis and antiproliferative activity of the new conjugates of E7010 and resveratrol as tubulin polymerization inhibitors||Organic and Biomolecular Chemistry||2016||ISSN 1477-0520|
|Fawzy, Iten M. et al.||Newly Designed and Synthesized Curcumin Analogs with in vitro Cytotoxicity and Tubulin Polymerization Activity||Chemical biology & drug design||2015||ISSN 1747--0285|
|Mu, Yan et al.||The novel tubulin polymerization inhibitor MHPT exhibits selective anti-tumor activity against rhabdomyosarcoma in vitro and in vivo||PLoS ONE||2015||ISSN 1932-6203|
|Xu, Qile et al.||Synthesis and biological evaluation of 3-alkyl-1,5-diaryl-1H-pyrazoles as rigid analogues of combretastatin A-4 with potent antiproliferative activity||PLoS ONE||2015||ISSN 1932-6203|
|Seashore-Ludlow, Brinton et al.||Harnessing connectivity in a large-scale small-molecule sensitivity dataset||Cancer Discovery||2015||ISSN 2159-8290|
|Remers, William A. et al.||Synthesis and Antitumor Activity of Heterocycles Related to Carbendazim||Journal of Heterocyclic Chemistry||2015||ISSN 1943--5193|
|Sheldon, Jonathon E. et al.||Photoswitchable anticancer activity via trans-cis isomerization of a combretastatin A-4 analog||Organic and Biomolecular Chemistry||2015||ISSN 1477-0520|
|Calles, Antonio et al.||Tivantinib (ARQ 197) efficacy is independent of MET inhibition in non-small-cell lung cancer cell lines||Molecular Oncology||2015||ISSN 1878-0261|
|Yan, Jun et al.||A novel synthetic compound exerts effective anti-tumour activity in vivo via the inhibition of tubulin polymerisation in A549 cells||Biochemical Pharmacology||2015||ISSN 1873-2968|
|Wu, Shaoyu et al.||Bis-cyclopropane analog of disorazole C1 is a microtubuledestabilizing agent active in ABCB1-overexpressing human colon cancer cells||Oncotarget||2015||ISSN 1949-2553|
|Shigehiro, Tsukasa et al.||Efficient Drug Delivery of Paclitaxel Glycoside: A Novel Solubility Gradient Encapsulation into Liposomes Coupled with Immunoliposomes Preparation||PLOS ONE||2014||ISSN 1932--6203|
|Choi, Bo Hwa et al.||Suprafenacine, an Indazole-Hydrazide Agent, Targets Cancer Cells Through Microtubule Destabilization||PLOS ONE||2014||ISSN 1932--6203|
|Senese, S. et al.||Chemical dissection of the cell cycle: Probes for cell biology and anti-cancer drug development||Cell Death and Disease||2014||ISSN 2041-4889|
|Lutz, Vanessa et al.||SAR studies on hydropentalene derivatives - Important core units of biologically active tetramic acid macrolactams and ptychanolides||Bioorganic and Medicinal Chemistry||2014||ISSN 1464-3391|
|Mei, Mei et al.||A new 2α,5α,10β,14β-tetraacetoxy-4(20),11-taxadiene (SIA) derivative overcomes paclitaxel resistance by inhibiting MAPK signaling and increasing paclitaxel accumulation in breast cancer cells||PLoS ONE||2014||ISSN 1932-6203|
|Bio, Moses et al.||Far-red light activatable, multifunctional prodrug for fluorescence optical imaging and combinational treatment||Journal of Medicinal Chemistry||2014||ISSN 1520-4804|
|Mäki-Jouppila, Jenni H.E. et al.||Centmitor-1, a novel acridinyl-acetohydrazide, possesses similar molecular interaction field and antimitotic cellular phenotype as rigosertib, on 01910.Na||Molecular Cancer Therapeutics||2014||ISSN 1538-8514|
|Zhang, Qiu et al.||Anti-tumor selectivity of a novel Tubulin and HSP90 dual-targeting inhibitor in non-small cell lung cancer models||Biochemical Pharmacology||2013||ISSN 1873-2968|
|Bio, Moses et al.||Site-specific and far-red-light-activatable prodrug of combretastatin A-4 using photo-unclick chemistry||Journal of Medicinal Chemistry||2013||ISSN 0022-2623|
|Zach, Frank et al.||The retinitis pigmentosa 28 protein FAM161A is a novel ciliary protein involved in intermolecular protein interaction and microtubule association||Human molecular genetics||2012||ISSN 1460--2083|
|Sidhaye, Venkataramana K. et al.||A Novel Role for Aquaporin-5 in Enhancing Microtubule Organization and Stability||PLOS ONE||2012||ISSN 1932--6203|
|Nguyen, Tam Luong et al.||Evading Pgp activity in drug-resistant cancer cells: A structural and functional study of antitubulin furan metotica compounds||Molecular Cancer Therapeutics||2012||ISSN 1535-7163|
|Fitzgerald, Daniel P. et al.||TPI-287, a new taxane family member, reduces the brain metastatic colonization of breast cancer cells||Molecular Cancer Therapeutics||2012||ISSN 1535-7163|
|Kawaratani, Yasuyuki et al.||New microtubule polymerization inhibitors comprising a nitrooxymethylphenyl group||Bioorganic and Medicinal Chemistry||2011||ISSN 0968-0896|
|Dyrager, Christine et al.||Inhibitors and promoters of tubulin polymerization: synthesis and biological evaluation of chalcones and related dienones as potential anticancer agents||Bioorganic & medicinal chemistry||2011||ISSN 1464--3391|
|Hwang, Ji Hwan et al.||Induction of tubulin polymerization and apoptosis in malignant mesothelioma cells by a new compound JBIR-23||Cancer letters||2011||ISSN 1872--7980|
|Kim, Insook et al.||Zinc stimulates tau S214 phosphorylation by the activation of Raf/mitogen-activated protein kinase-kinase/extracellular signal-regulated kinase pathway||NeuroReport||2011||ISSN 0959-4965|
|Oussenko, Irina A. et al.||Effect of on 01910.Na, an anticancer mitotic inhibitor, on cell-cycle progression correlates with RanGAP1 hyperphosphorylation||Cancer Research||2011||ISSN 0008-5472|
Answer 1:Cytoskeleton's Biochem Kits are an extremely quick way to become familiar with some of the most useful in vitro and in vivo assays for assaying different aspects of cytoskeletal biochemistry and signal transduction. These kits come with all the reagents needed for your assay as well as detailed instruction on how to use them, so you will be ready to do your assays as soon as you have the kits! Each kit is carefully quality controlled and very user friendly and will therefore save time and money even for experienced cytoskeletal researchers. Furthermore, Cytoskeleton, Inc. guarantees that the cost of each kit is substantially less than the sum of the component parts purchased separately. Cytoskeleton, Inc. provides Biochem Kits for actin, tubulin, G-protein activation, G-protein signal transduction and molecular motor research.
If you are new to the field of cytoskeletal research, the Biochem Kits will prove to be an invaluable introductory aid, providing many useful technical tips. For example, if you are new to the field of microtubule research, you may not know that tubulin requires GTP, Mg2+ and >50 mM PIPES for stability and that taxol (a microtubule stabilizing drug) added too quickly will cause aberrant tubulin arrangements to form. If any of these points are overlooked, the results could lead to inappropriate experimental interpretation.Biochem kits are designed to overcome this bottleneck in research.
Answer 2:Cytoskeleton, Inc. has a variety of kits that can help the researcher examine how recombinant proteins, drugs or even cell lysates either interact and/or affect the function of cytoskeletal proteins including monomers and polymers of actin and tubulin.
Some examples of kits specifically designed for this purpose include the actin binding protein assay Biochem Kit (Cat. # BK001 or BK013) and the tubulin binding protein assay Biochem Kit (Cat. # BK029).
Actin binding protein assay Biochem kit (Cat. # BK001 or BK013): This kit provides clear instructions and all the reagents and controls necessary to determine the ability of a given protein to bind to actin monomers versus actin filaments. F-actin binding can be measured by using a spin down assay where centrifugation is used to separate F-actin from G-actin by differential sedimentation. This kit can be used to determine (i) whether a test protein binds F-actin or affects the equilibrium between G-actin and F-actin, (ii) whether a test protein has G-actin sequestering or F-actin polymerization enhancing activity or (iii) whether a test protein of interest can bundle F-actin. We suggest coupling this assay with the Actin polymerization assay Biochem kit in order to obtain a more complete profile of your potential actin binding protein.
Tubulin binding protein assay Biochem Kit(Cat. # BK029):This kit provides clear instructions and all the reagents and controls necessary to determine the ability of a given protein to bind to tubulin monomers versus tubulin polymers (microtubules). Tubulin binding can be measured by using a spin down assay where centrifugation is used to separate microtubules from tubulin monomers by differential sedimentation. This kit can be used to: (i) identify novel microtubule associated proteins (MAPs), (ii) confirm in vivodata suggesting a given protein is a MAP, (iii) characterize MAPs, (iv) identify/characterize MAP regulating proteins or (v) identify/characterize compounds that inhibit MAP binding to microtubules. We suggest coupling this assay with the tubulin polymerization assay Biochem Kit (Cat. # BK006P or BK011P) in order to obtain a more complete profile of your potential tubulin binding protein.
Actin polymerization assay Biochem kit (Cat. # BK003): This kit uses modified actin to follow polymerization kinetics. There are many types of actin binding proteins, including nucleating, capping, severing, side-binding and monomer sequestering actin binding proteins. All of these can be characterized by their effect on one or more of the three stages of actin polymerization: nucleation, growth and steady-state equilibrium. A compound’s effects on depolymerization can also be evaluated. Cytoskeleton has adapted this powerful assay into a user friendly Biochem kit format.
Tubulin polymerization assay Biochem kit (Cat. # BK006P or BK001P): We offer both absorbance-based and fluorescence-based tubulin polymerization kits to follow polymerization kinetics. The absorbance-based kit (Cat. # BK006P) takes advantage of the fact that light is scattered by microtubules to an extent that is proportional to the concentration of microtubule polymer. The fluorescence-based kit (Cat. # BK011P) measures polymerization as a function of fluorescence enhancement following the incorporation of a fluorescent reporter into microtubules as polymerization occurs. There are many proteins and drugs that either enhance or inhibit polymerization or depolymerization. These compounds can be characterized by their effect on one or more of the three stages of tubulin polymerization: nucleation, growth, and steady-state equilibrium. A compound’s effects on depolymerization can also be evaluated. Cytoskeleton has adapted these powerful assays into user friendly Biochem Kit formats.