Small G-protein

Cytoskeleton provides a range of antibodies raised to the Rho family of small G-proteins. They have exceptional isoform specificity and each are provided with human platelet extract as a positive control. For more information on each antibody please click on the products below.

 

In addition, Cytoskeleton has developed a unique line of fluorescent phalloidins with improved brightness and stability compared to other conjugates such as Alexa fluor and Cy dyes, for more information see the Acti-stain information page.

Anti-Rac1 Western blot Comparison

Q:  How do I know whether the Cdc42 monoclonal antibody is good for Western blotting and IF?

A: The datasheet (Cat. # ACD03) and the website's individual product page (ACD03) descibe the applications for all Cytoskeleton's antibodies. The Cdc42 antibody (Cat. # ACD03) was raised against a peptide sequence of human Cdc42 (amino acids 123-141).  The Cdc42 antibody will not cross-react with a ten-fold excess of RhoA, RhoB, RhoC, Rac1, Rac2 or Rac3 proteins. Non-specific bands have been observed at higher molecular weight in about 50% of cell lines tested.  The antibody has been tested by western blotting and immunocytochemistry.  Please see the Citations tab for references that have used this antibody. 

 

Q:  Can I test a sample of the RhoA monoclonal antibody?

A:  All of Cytoskeleton’s antibodies are rigorously quality-controlled and tested under the conditions described in the product datasheet.  In the rare event of a problem with an antibody, please contact our technical service group (tservice@cytoskeleton.com) for assistance.  Free samples of the RhoA antibody (cat# ARH03) are not available.  However, please contact Cytoskeleton’s customer service department (303-322-2254 or cserve@cytoskeleton.com) to check on any special discount offers. 

 

For more information, click on the Document tab above to see the datasheet.