Cdc42 is one of most extensively studied member of Rho GTPase family. This family is involved in a wide range of cellular responses, including cytoskeletal reorganization, regulation of transcription, cell migration, cellular transformation and metastasis. Cdc42 is expressed in a large number of different cell types.
Product Uses Include
This antibody is recommended for detection of Cdc42 in human, mouse, rat or other extracts. The following protocols have been tested with this antibody:
The anti-Cdc42 antibody (Cat. # ACD03) is a mouse monoclonal antibody that only recognizes Cdc42, not Rac1, Rac2, Rac3, RhoA, RhoB, RhoC or H-Ras. Other commercially available Cdc42 antibodies recognize one or more of these close isoforms Platelet extract (Cat. # EXT01) is included as a positive control. ACD03 is supplied as a lyophilized white powder.
Figure 1: Western blot analysis of purified protein and cell extracts using ACD03 as primary antibody. Recombinant small G-proteins and tissue extracts were separated by SDS-PAGE and transferred to PVDF membrane. ACD03 was diluted to 250 ng/ml (1:1000) in PBST plus 0.1% non-fat milk powder and Western analysis was as described in the Methods section of the datasheet. Lane 1; 25 ng Cdc42-6xHIS, Lane 2; 250 ng RhoA-6xHIS, Lane 3; 250 ng Rac1-6xHIS, Lane 4; 40 µg Swiss 3T3 cell extract, Lane 5; 40 µg bovine brain extract, Lane 6; 40 µg human platelet extract. Note: Cdc42-6xHIS runs at 25kDal whereas the native Cdc42 runs at 22kDal.
Table 1 - Reactivity of other commercially available Cdc42 antibodies
Cytoskeleton, Inc. (Cat. # ACD03)
Santa Cruz (Cat. #sc-87-G)
Cell Signalling (Cat. # 2462)
NT = Not tested
|Kamata, Tamihiro et al.||Statins mediate anti- and pro-tumourigenic functions by remodelling the tumour microenvironment||DMM Disease Models and Mechanisms||2022||ISSN 1754-8411|
|Barbera, Stefano et al.||The C-type lectin CD93 controls endothelial cell migration via activation of the Rho family of small GTPases||Matrix Biology||2021||ISSN 1569-1802|
|Howden, Jake D. et al.||α2β1 integrins spatially restrict Cdc42 activity to stabilise adherens junctions||BMC biology||2021||ISSN 1741-7007|
|Li, Jingxuan et al.||Eva1a ameliorates atherosclerosis by promoting re-endothelialization of injured arteries via Rac1/Cdc42/Arpc1b||Cardiovascular Research||2021||ISSN 1755-3245|
|Jayabal, Panneerselvam et al.||NELL2-cdc42 signaling regulates BAF complexes and Ewing sarcoma cell growth||Cell Reports||2021||ISSN 2211-1247|
|Hosseini, Kamran et al.||EMT-Induced Cell-Mechanical Changes Enhance Mitotic Rounding Strength||Advanced Science||2020||ISSN 2198-3844|
|Gorisse, Laetitia et al.||Ubiquitination of the scaffold protein IQGAP1 diminishes its interaction with and activation of the Rho GTPase CDC42||Journal of Biological Chemistry||2020||ISSN 1083-351X|
|Gu, Jiawen et al.||Rho-GEF trio regulates osteoclast differentiation and function by Rac1/Cdc42||Experimental Cell Research||2020||ISSN 1090-2422|
|Oni, Tobiloba E. et al.||SOAT1 promotes mevalonate pathway dependency in pancreatic cancer||Journal of Experimental Medicine||2020||ISSN 1540-9538|
|Vestre, Katharina et al.||Rab6 regulates cell migration and invasion by recruiting Cdc42 and modulating its activity||Cellular and Molecular Life Sciences||2019||ISSN 1420-9071|
|Singh, Rajesh K. et al.||Dynamic Actin Reorganization and Vav/Cdc42-Dependent Actin Polymerization Promote Macrophage Aggregated LDL (Low-Density Lipoprotein) Uptake and Catabolism||Arteriosclerosis, Thrombosis, and Vascular Biology||2019||ISSN 1524-4636|
|Dupraz, Sebastian et al.||RhoA Controls Axon Extension Independent of Specification in the Developing Brain||Current Biology||2019||ISSN 0960-9822|
|Huang, Yuxing et al.||Arp2/3-branched actin maintains an active pool of GTP-RhoA and controls RhoA abundance||Cells||2019||ISSN 2073-4409|
|MacKeil, Jodi L. et al.||Phosphodiesterase 3B (PDE3B) antagonizes the anti-angiogenic actions of PKA in human and murine endothelial cells||Cellular Signalling||2019||ISSN 1873-3913|
|Li, Cao et al.||Regulation of staphylococcus aureus infection of macrophages by CD44, reactive oxygen species, and acid sphingomyelinase||Antioxidants and Redox Signaling||2018||ISSN 1557-7716|
|Stypulkowski, Ewa et al.||The depalmitoylase APT1 directs the asymmetric partitioning of Notch and Wnt signaling during cell division||Science Signaling||2018||ISSN 1937-9145|
|Kawasaki, Natsumi et al.||TUFT1 interacts with RABGAP1 and regulates mTORC1 signaling||Cell Discovery||2018||ISSN 2056-5968|
|Ghézali, Grégory et al.||Connexin 30 controls astroglial polarization during postnatal brain development||Development (Cambridge)||2018||ISSN 1477-9129|
|Vodicska, Barbara et al.||MISP regulates the IQGAP1/Cdc42 complex to collectively orchestrate spindle orientation and mitotic progression||Scientific Reports||2018||ISSN 2045-2322|
|Wu, Nan et al.||RCC2 over-expression in tumor cells alters apoptosis and drug sensitivity by regulating Rac1 activation||BMC Cancer||2018||ISSN 1471-2407|
|Lam, Jonathan G.T. et al.||Host cell perforation by listeriolysin O (LLO) activates a Ca2+-dependent cPKC/Rac1/Arp2/3 signaling pathway that promotes Listeria monocytogenes internalization independently of membrane resealing||Molecular Biology of the Cell||2018||ISSN 1939-4586|
|Liu, Xiaolei et al.||Rasip1 controls lymphatic vessel lumen maintenance by regulating endothelial cell junctions||Development (Cambridge)||2018||ISSN 1477-9129|
|Peretti, Amanda S. et al.||The R-Enantiomer of Ketorolac Delays Mammary Tumor Development in Mouse Mammary Tumor Virus-Polyoma Middle T Antigen (MMTV-PyMT) Mice||American Journal of Pathology||2018||ISSN 1525-2191|
|Dudvarski Stanković, Nevenka et al.||EGFL7 enhances surface expression of integrin α 5 β 1 to promote angiogenesis in malignant brain tumors||EMBO Molecular Medicine||2018||ISSN 1757--4676|
|Saito, Masaki et al.||Tctex‐1 controls ciliary resorption by regulating branched actin polymerization and endocytosis||EMBO reports||2017||ISSN 1469--221X|
|Bucka, Kenneth B. et al.||Local Arp2/3-dependent actin assembly modulates applied traction force during apCAM adhesion site maturation||Molecular Biology of the Cell||2017||ISSN 1939-4586|
|Chang, Ting Ya et al.||Paxillin facilitates timely neurite initiation on soft-substrate environments by interacting with the endocytic machinery||eLife||2017||ISSN 2050-084X|
|Takeuchi, Hiroki et al.||Intracellular periodontal pathogen exploits recycling pathway to exit from infected cells||Cellular Microbiology||2016||ISSN 1462-5822|
|Seidelin, Jakob Benedict et al.||Cellular inhibitor of apoptosis protein 2 controls human colonic epithelial restitution, migration, and Rac1 activation||American Journal of Physiology - Gastrointestinal and Liver Physiology||2015||ISSN 1522-1547|
|Thomas, Audrey et al.||Involvement of the Rac1-IRSp53-Wave2-Arp2/3 Signaling Pathway in HIV-1 Gag Particle Release in CD4 T Cells||Journal of Virology||2015||ISSN 0022--538X|
|Liu, Chunqiao et al.||Null and hypomorph Prickle1 alleles in mice phenocopy human Robinow syndrome and disrupt signaling downstream of Wnt5a||Biology Open||2014||ISSN 2046-6390|
|David, Muriel D. et al.||The RhoGAP ARHGAP19 controls cytokinesis and chromosome segregation in T lymphocytes||Journal of Cell Science||2014||ISSN 0021-9533|
|Valtcheva, Nadejda et al.||The orphan adhesion G protein-coupled receptor GPR97 regulates migration of lymphatic endothelial cells via the small GTPases RhoA and Cdc42||Journal of Biological Chemistry||2013||ISSN 0021-9258|
|Barrio, Laura et al.||TLR4 Signaling Shapes B Cell Dynamics via MyD88-Dependent Pathways and Rac GTPases||The Journal of Immunology||2013||ISSN 0022--1767|
|Steele, Brian M. et al.||WNT-3a modulates platelet function by regulating small GTPase activity||FEBS Letters||2012||ISSN 0014-5793|
|Mercer, Jason et al.||Vaccinia virus strains use distinct forms of macropinocytosis for host-cell entry||Proceedings of the National Academy of Sciences of the United States of America||2010||ISSN 0027-8424|
Question 1: What is the antigen that this antibody was raised against?
Answer 1: The antibody has been raised against a peptide sequence of human Cdc42 (amino acids 128-138).
Question 2: What species has this antibody been tested against?
Answer 2: This antibody is recommended for western blot detection of Cdc42 in human, mouse, rat and bovine cell/tissue extracts.
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