Validated for:

• Western blot-WB
• Immunofluorescence-IF

Anti-Rac1 antibody (ARC03) was raised against a proprietary peptide sequence of human Rac1 (carboxy terminal amino acids). It specifically recognizes Rac1 (21-23 kD). ARC03 is purified by Protein G affinity chromatography.

Host/isotype/clone:

• Mouse/IgG2b
• C7H2

Reactivity:

• Broad cross-species reactivity, including mammalian, reptile, avian, and amphibian sources

Amount of material:

• ARC03: 2 x 100 µl when reconstituted (70-90 µg per tube)
• ARC03-S: 1 x 25 µl when reconstituted (17.5-22.5 µg per tube)

Working concentrations:

• WB 1:500 dilution 1.4-1.8 µg/ml final 100 ml working volume - full size Ab

Formulation

• Supplied as a lyophilized powder
• Buffer composition after reconstitution in nanopure water: 100 mM PIPES pH7.0, 1% sucrose, 0.5% dextran

Sensitivity:

• Detects 1 ng recombinant Rac1
• In native samples, a ~21 kD Rac1 band is clearly visible in 50 µg of platelet extract

Specificity

• High selectivity: Detects 1 ng of recombinant Rac1 and does not detect 50 ng of Rac 2, Rac 3, or Cdc42 —demonstrating a ≥50-fold specificity.
• Across a panel of cell lysates analyzed via Western blotting, the only detectable band is at ~21 kDa, corresponding to Rac1.