Anti-Rac1: mouse Mab

Anti-Rac1: mouse Mab
$0.00

Introduction

Rac1 is one of most extensively studied member of Rho GTPase family. This family is involved in a wide range of cellular responses, including cytoskeletal reorganization, regulation of transcription, cell migration, cellular transformation and metastasis. Rac1 is expressed in a large number of different cell types.

 

Product Uses Include

This antibody is recommended for detection of Rac1 in human, mouse, rat or other extracts.  The following protocols have been tested with this antibody:

  • Western blot:  recommended  
  • Immunocytochemistry:  recommended

 

RRID

AB_10709099

Material

The anti-Rac1 antibody (Cat. # ARC03) is a mouse monoclonal antibody that only recognizes Rac1, not Rac2, Rac3, Cdc42 or other small GTPases. Other commercially available Rac1 antibodies recognize Rac2 and 3, some even recognize Cdc42 (Table 1 and Figure 1).  Platelet extract (Cat. # EXT01) is included as a positive control. ARC03 is supplied as a lyophilized white powder.

Figure 1 - Comparison of other commercially available antibodies by western analysis.

Blots

Figure 1 legend - Western blot of purified Rac1, 2, 3, Cdc42 and platelet extracts probed with anti-Rac1 monoclonal antibody (Cat. # ARC03) and other commercially availabe Rac1 antibodies. The detection of 25 ng His-Rac1 ( lane 1), His-Rac2 (lane 2), GST-Rac3 (lane 3), GST-Cdc42 (lane 4) and 50 µg of platelet extract (lane 5). ARC03 does not cross-react with Rac2, 3 or Cdc42, while all other commercially available Rac1 antibody crossreact with GTPases other than Rac1. The blot was probed with a 1 µg/ml (1:500) dilution of ARC03. Other blots were probed according to manufacturers’ protocols.

 Table 1 - Reactivity of other commercially available Rac antibodies

Rac1

Rac2

Rac3

Cdc42

Cytoskeleton, Inc. (Cat. # ARC03)

Yes

No

No

No 

BD Biosciences (Cat. #610650)

Yes

Yes

Yes

Yes

Santa Cruz  (Cat. #sc-95)

Yes

No

Yes

Yes

Upstate  (Cat. # 05-389)

Yes

No

Yes

No 



Immunocytochemistry

Figure 2 - Cell staining by ARC03 using immunocytochemistry.

Cell_image_ARC03

Figure 2 - Immunofluorescence image of mouse Swiss 3T3 cells stained with Anti-Rac1  monoclonal antibody (Cat. # ARC03). Swiss 3T3 cells were grown to semi-confluency and fixed with methanol. Immunofluorescence staining using 1 µg/ml (1:20 dilution) of ARC03 antibody is shown (red). The primary antibody was detected with a 1:500 dilution of goat anti-mouse rhodamine conjugated antibody. Photograph was taken with a 100X objective lens.

Other associated products:

Anti-RhoA monoclonal antibody (Cat.# ARH03)

Anti-Cdc42 monoclonal antibody (Cat.# ACD03)

Cdc42 G-LISA Activation Assay (Cat.# BK127)

RhoA G-LISA Activation Assay (Cat.# BK124)

Rac1 G-LISA Activation Assay (Cat.# BK126)

Rac1,2,3 G-LISA Activation Assay (Cat.# BK125)

For product Datasheets and MSDSs please click on the PDF links below.   For additional information, click on the FAQs tab above or contact our Technical Support department at tservice@cytoskeleton.com

AuthorTitleJournalYearArticle Link
Sha, Zhilin et al.Hepatic Ischemia-reperfusion Injury in Mice was Alleviated by Rac1 Inhibition – More Than Just ROS-inhibitionJournal of Clinical and Translational Hepatology2022ISSN 2310-8819
Weddle, Erin A. et al.The type three secretion system effector protein IpgB1 promotes Shigella flexneri cell-to-cell spread through double-membrane vacuole escapePLoS Pathogens2022ISSN 1553-7374
An, Ni et al.Inhibition of Rac1 attenuates radiation-induced lung injury while suppresses lung tumor in miceCell Death Discovery2022ISSN 2058-7716
Romano, Roberta et al.Alteration of the late endocytic pathway in Charcot-Marie-Tooth type 2B diseaseCellular and molecular life sciences : CMLS2021ISSN 1420--9071
Arandjelovic, Sanja et al.ELMO1 signaling is a promoter of osteoclast function and bone lossNature Communications2021ISSN 2041-1723
Pickering, K. A. et al.A RAC-GEF network critical for early intestinal tumourigenesisNature Communications2021ISSN 2041-1723
Li, Jingxuan et al.Eva1a ameliorates atherosclerosis by promoting re-endothelialization of injured arteries via Rac1/Cdc42/Arpc1bCardiovascular Research2021ISSN 1755-3245
Afanasyeva, Elena A. et al.Kalirin-RAC controls nucleokinetic migration in ADRN-type neuroblastomaLife Science Alliance2021ISSN 2575-1077
Romano, Roberta et al.Alteration of the late endocytic pathway in Charcot–Marie–Tooth type 2B diseaseCellular and Molecular Life Sciences2021ISSN 1420-9071
Kim, Yeon Sun et al.Cannabinoid and planar cell polarity signaling converges to direct placentationProceedings of the National Academy of Sciences of the United States of America2021ISSN 1091-6490
Kalargyrou, Aikaterini A et al.Nanotube‐like processes facilitate material transfer between photoreceptorsEMBO reports2021ISSN 1469--221X
Floerchinger, Alessia et al.Optimizing metastatic-cascade-dependent Rac1 targeting in breast cancer: Guidance using optical window intravital FRET imagingCell Reports2021ISSN 2211-1247
Miroshnikova, Yekaterina A. et al.Calcium signaling mediates a biphasic mechanoadaptive response of endothelial cells to cyclic mechanical stretchMolecular Biology of the Cell2021ISSN 1939-4586
Fulmer, Diana et al.Desert hedgehog-primary cilia cross talk shapes mitral valve tissue by organizing smooth muscle actinDevelopmental Biology2020ISSN 1095-564X
Kogler, Melanie et al.HACE1 prevents lung carcinogenesis via inhibition of RAC-Family GTPasesCancer Research2020ISSN 1538-7445
Gorisse, Laetitia et al.Ubiquitination of the scaffold protein IQGAP1 diminishes its interaction with and activation of the Rho GTPase CDC42Journal of Biological Chemistry2020ISSN 1083-351X
Rodríguez-Fdez, Sonia et al.Vav2 catalysis-dependent pathways contribute to skeletal muscle growth and metabolic homeostasisNature Communications2020ISSN 2041-1723
Lorenzo-Martín, L. Francisco et al.Vav2 pharmaco-mimetic mice reveal the therapeutic value and caveats of the catalytic inactivation of a Rho exchange factorOncogene2020ISSN 1476-5594
D'Amore, Claudio et al.“Janus” efficacy of CX-5011: CK2 inhibition and methuosis induction by independent mechanismsBiochimica et Biophysica Acta - Molecular Cell Research2020ISSN 1879-2596
Fray, Michael A. et al.Vav2 lacks Ca2+entry-promoting scaffolding functions unique to Vav1 and inhibits T cell activation via Cdc42Journal of Cell Science2020ISSN 1477-9137
Tilve, Sharada et al.Phospholipid phosphatase related 1 (PLPPR1) increases cell adhesion through modulation of Rac1 activityExperimental Cell Research2020ISSN 1090-2422
Draicchio, Fulvia et al.Integrin-associated ILK and PINCH1 protein content are reduced in skeletal muscle of maintenance haemodialysis patientsJournal of Physiology2020ISSN 1469-7793
Hervieu, Alexia et al.A PI3K- And GTPase-independent Rac1-mTOR mechanism mediates MET-driven anchorage-independent cell growth but not migrationScience Signaling2020ISSN 1937-9145
Lo, Louisa Hoi Ying et al.The Protein Arginine Methyltransferase PRMT8 and Substrate G3BP1 Control Rac1-PAK1 Signaling and Actin Cytoskeleton for Dendritic Spine MaturationCell Reports2020ISSN 2211-1247
Frye, Maike et al.Ephrinb2-ephb4 signalling provides rho-mediated homeostatic control of lymphatic endothelial cell junction integrityeLife2020ISSN 2050-084X
Guo, Yaxiu et al.Cytotoxic necrotizing factor 1 promotes bladder cancer angiogenesis through activating RhoCFASEB Journal2020ISSN 1530-6860
Won, So Yeon et al.Fibulin 5, a human Wharton's jelly-derived mesenchymal stem cells-secreted paracrine factor, attenuates peripheral nervous system myelination defects through the Integrin-RAC1 signaling axisStem Cells2020ISSN 1549-4918
Löber, Jens et al.Vav family proteins constitute disparate branching points for distinct BCR signaling pathwaysEuropean Journal of Immunology2020ISSN 1521-4141
Goulielmaki, Evi et al.Tissue-infiltrating macrophages mediate an exosome-based metabolic reprogramming upon DNA damageNature Communications2020ISSN 2041-1723
Balaji Ragunathrao, Vijay Avin et al.Sphingosine-1-Phosphate Receptor 1 Activity Promotes Tumor Growth by Amplifying VEGF-VEGFR2 Angiogenic SignalingCell Reports2019ISSN 2211-1247
Singh, Rajesh K. et al.Dynamic Actin Reorganization and Vav/Cdc42-Dependent Actin Polymerization Promote Macrophage Aggregated LDL (Low-Density Lipoprotein) Uptake and CatabolismArteriosclerosis, Thrombosis, and Vascular Biology2019ISSN 1524-4636
Wong, Bin Sheng et al.A direct podocalyxin–dynamin-2 interaction regulates cytoskeletal dynamics to promote migration and metastasis in pancreatic cancer cellsCancer Research2019ISSN 1538-7445
Ramadass, Mahalakshmi et al.The trafficking protein JFC1 regulates Rac1-GTP localization at the uropod controlling neutrophil chemotaxis and in vivo migrationJournal of Leukocyte Biology2019ISSN 1938-3673
Chellini, Lidia et al.Regulation of extracellular matrix degradation and metastatic spread by IQGAP1 through endothelin-1 receptor signalling in ovarian cancerMatrix Biology2019ISSN 1569-1802
Huang, Yuxing et al.Arp2/3-branched actin maintains an active pool of GTP-RhoA and controls RhoA abundanceCells2019ISSN 2073-4409
Mills, Shirley C. et al.Rac1 plays a role in CXCL12 but not CCL3-induced chemotaxis and Rac1 GEF inhibitor NSC23766 has off target effects on CXCR4Cellular Signalling2018ISSN 1873-3913
Olthoff, John T. et al.Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscleNature Communications2018ISSN 2041-1723
Wu, Yuqing et al.Mycobacterial infection is promoted by neutral sphingomyelinase 2 regulating a signaling cascade leading to activation of β1-integrinCellular Physiology and Biochemistry2018ISSN 1421-9778
Hayes, Madeline N. et al.Vangl2/RhoA Signaling Pathway Regulates Stem Cell Self-Renewal Programs and Growth in RhabdomyosarcomaCell Stem Cell2018ISSN 1875-9777
Dudvarski Stanković, Nevenka et al. EGFL7 enhances surface expression of integrin α 5 β 1 to promote angiogenesis in malignant brain tumors EMBO Molecular Medicine2018ISSN 1757--4676
Stierwalt, Harrison D. et al.Insulin-stimulated Rac1-GTP binding is not impaired by palmitate treatment in L6 myotubesPhysiological Reports2018ISSN 2051-817X
Raun, Steffen H. et al.Rac1 muscle knockout exacerbates the detrimental effect of high-fat diet on insulin-stimulated muscle glucose uptake independently of AktJournal of Physiology2018ISSN 1469-7793
Murillo, Miguel M. et al.Disruption of the Interaction of RAS with PI 3-Kinase Induces Regression of EGFR-Mutant-Driven Lung CancerCell Reports2018ISSN 2211-1247
Wu, Nan et al.RCC2 over-expression in tumor cells alters apoptosis and drug sensitivity by regulating Rac1 activationBMC Cancer2018ISSN 1471-2407
Cheng, Yuyan et al.TNFα disrupts blood brain barrier integrity to maintain prolonged depressive-like behavior in miceBrain, Behavior, and Immunity2018ISSN 1090-2139
Liu, Xiaolei et al.Rasip1 controls lymphatic vessel lumen maintenance by regulating endothelial cell junctionsDevelopment (Cambridge)2018ISSN 1477-9129
Peretti, Amanda S. et al.The R-Enantiomer of Ketorolac Delays Mammary Tumor Development in Mouse Mammary Tumor Virus-Polyoma Middle T Antigen (MMTV-PyMT) MiceAmerican Journal of Pathology2018ISSN 1525-2191
Li, Cao et al.Regulation of staphylococcus aureus infection of macrophages by CD44, reactive oxygen species, and acid sphingomyelinaseAntioxidants and Redox Signaling2018ISSN 1557-7716
Poudel, Kumud R. et al.Competition between TIAM1 and Membranes Balances Endophilin A3 Activity in Cancer MetastasisDevelopmental Cell2018ISSN 1878-1551
Chen, Yuxiao et al.Restoring striatal WAVE-1 improves maze exploration performance of GluN1 knockdown micePLoS ONE2018ISSN 1932-6203
Lam, Jonathan G.T. et al.Host cell perforation by listeriolysin O (LLO) activates a Ca2+-dependent cPKC/Rac1/Arp2/3 signaling pathway that promotes Listeria monocytogenes internalization independently of membrane resealingMolecular Biology of the Cell2018ISSN 1939-4586
Nakamuta, Shinichi et al.Dual role for DOCK7 in tangential migration of interneuron precursors in the postnatal forebrainJournal of Cell Biology2017ISSN 1540-8140
Sylow, Lykke et al.Rac1 and AMPK account for the majority of muscle glucose uptake stimulated by ex vivo contraction but not in vivo exerciseDiabetes2017ISSN 1939-327X
Barrera-Chimal, Jonatan et al.Benefit of mineralocorticoid receptor antagonism in AKI: Role of vascular smooth muscle Rac1Journal of the American Society of Nephrology2017ISSN 1533-3450
Chang, Ting Ya et al.Paxillin facilitates timely neurite initiation on soft-substrate environments by interacting with the endocytic machineryeLife2017ISSN 2050-084X
Bucka, Kenneth B. et al.Local Arp2/3-dependent actin assembly modulates applied traction force during apCAM adhesion site maturationMolecular Biology of the Cell2017ISSN 1939-4586
Margiotta, Azzurra et al.Rab7a regulates cell migration through Rac1 and vimentinBiochimica et Biophysica Acta - Molecular Cell Research2017ISSN 1879-2596
Gao, Jie et al.The E3 ubiquitin ligase IDOL regulates synaptic ApoER2 levels and is important for plasticity and learningeLife2017ISSN 2050-084X
Tarr, Joseph T. et al.The pivotal role of CCN2 in mammalian palatogenesisJournal of Cell Communication and Signaling2017ISSN 1873-961X
Atala, AnthonyRe: AKT-Mediated Stabilization of Histone Methyltransferase WHSC1 Promotes Prostate Cancer MetastasisThe Journal of urology2017ISSN 1527-3792
Sabbir, Mohammad G. et al.Dlc1 interaction with non-muscle myosin heavy chain II-A (Myh9) and Rac1 activationBiology Open2016ISSN 2046-6390
Gervais, Elise M. et al.Par-1b is required for morphogenesis and differentiation of myoepithelial cells during salivary gland developmentOrganogenesis2016ISSN 1555-8592
Moodley, Serisha et al.Stimulus-dependent dissociation between XB130 and Tks5 scaffold proteins promotes airway epithelial cell migrationOncotarget2016ISSN 1949-2553
Cui, Hong Yong et al.CD147 regulates cancer migration via direct interaction with Annexin A2 and DOCK3-β-catenin-WAVE2 signalingOncotarget2016ISSN 1949-2553
Wang, Hui et al.LOVTRAP: An optogenetic system for photoinduced protein dissociationNature Methods2016ISSN 1548-7105
Müller, Alena et al.Comparative study of antitumor effects of bromelain and papain in human cholangiocarcinoma cell linesInternational Journal of Oncology2016ISSN 1791-2423
Cao, Xuan et al.A phosphorylation switch controls the spatiotemporal activation of Rho GTPases in directional cell migrationNature Communications2015ISSN 2041-1723
Thomas, Audrey et al.Involvement of the Rac1-IRSp53-Wave2-Arp2/3 Signaling Pathway in HIV-1 Gag Particle Release in CD4 T CellsJournal of Virology2015ISSN 0022--538X
Sun, Ling Ling et al.A three-gene signature and clinical outcome in esophageal squamous cell carcinomaInternational Journal of Cancer2015ISSN 1097-0215
Seidelin, Jakob Benedict et al.Cellular inhibitor of apoptosis protein 2 controls human colonic epithelial restitution, migration, and Rac1 activationAmerican Journal of Physiology - Gastrointestinal and Liver Physiology2015ISSN 1522-1547
Ko, Hyun Kyung et al.Suppression of chemotaxis by SSeCKS via scaffolding of phosphoinositol phosphates and the recruitment of the Cdc42 GEF, frabin, to the leading edgePLoS ONE2014ISSN 1932-6203
Liu, Chunqiao et al.Null and hypomorph Prickle1 alleles in mice phenocopy human Robinow syndrome and disrupt signaling downstream of Wnt5aBiology Open2014ISSN 2046-6390
Ezzine, Aymen et al.Recombinant form of mammalian gp91phox is active in the absence of p22phoxBiochemical Journal2014ISSN 1470-8728
Stanley, Amanda C. et al.The Rho GTPase Rac1 is required for recycling endosome-mediated secretion of TNF in macrophagesImmunology and Cell Biology2014ISSN 1440-1711
Mackay, Joanna L. et al.Simultaneous and independent tuning of RhoA and Rac1 activity with orthogonally inducible promotersIntegrative Biology (United Kingdom)2014ISSN 1757-9708
Hori, Kei et al.Cytoskeletal Regulation by AUTS2 in Neuronal Migration and NeuritogenesisCell Reports2014ISSN 2211-1247
Galic, Milos et al.Dynamic recruitment of the curvature-sensitive protein ArhGAP44 to nanoscale membrane deformations limits exploratory filopodia initiation in neuronseLife2014ISSN 2050-084X
Tong, Junfeng et al.Phosphorylation of Rac1 T108 by extracellular signal-regulated kinase in response to epidermal growth factor: a novel mechanism to regulate Rac1 functionMolecular and cellular biology2013ISSN 1098--5549
Huelsenbeck, Stefanie C. et al.Expression and cytoprotective activity of the small GTPase RhoB induced by the Escherichia coli cytotoxic necrotizing factor 1International Journal of Biochemistry and Cell Biology2013ISSN 1878-5875
Golden, Sam A. et al.Epigenetic regulation of RAC1 induces synaptic remodeling in stress disorders and depressionNature Medicine2013ISSN 1078-8956
Buranda, Tione et al.Rapid parallel flow cytometry assays of active GTPases using effector beadsAnalytical Biochemistry2013ISSN 1096-0309
Floc'h, Audrey Le et al.Annular PIP3 accumulation controls actin architecture and modulates cytotoxicity at the immunological synapseJournal of Experimental Medicine2013ISSN 1540-9538
Perez, Sylvia E. et al.Rac1b increases with progressive tau pathology within cholinergic nucleus basalis neurons in alzheimer's diseaseAmerican Journal of Pathology2012ISSN 0002-9440
Lehtinen, Laura et al.15-Hydroxyprostaglandin dehydrogenase associates with poor prognosis in breast cancer, induces epithelial-mesenchymal transition, and promotes cell migration in cultured breast cancer cellsJournal of Pathology2012ISSN 0022-3417
Wong, Hon Kit et al.Merlin/NF2 regulates angiogenesis in schwannomas through a Rac1/semaphorin 3F-dependent mechanismNeoplasia2012ISSN 1476-5586
Härmä, V. et al.Lysophosphatidic acid and sphingosine-1-phosphate promote morphogenesis and block invasion of prostate cancer cells in three-dimensional organotypic modelsOncogene2012ISSN 0950-9232
Vidaki, Marina et al.Rac1-dependent cell cycle exit of MGE precursors and GABAergic interneuron migration to the cortexCerebral cortex (New York, N.Y. : 1991)2012ISSN 1460--2199
Cheadle, Lucas et al.The novel synaptogenic protein farp1 links postsynaptic cytoskeletal dynamics and transsynaptic organizationJournal of Cell Biology2012ISSN 0021-9525
Orange, Jordan S. et al.IL-2 induces a WAVE2-dependent pathway for actin reorganization that enables WASp-independent human NK cell functionJournal of Clinical Investigation2011ISSN 0021-9738
Itokowa, Takashi et al.Osteoclasts lacking rac2 have defective chemotaxis and resorptive activityCalcified Tissue International2011ISSN 0171-967X
Khan, Omar M. et al.Geranylgeranyltransferase type I (GGTase-I) deficiency hyperactivates macrophages and induces erosive arthritis in miceJournal of Clinical Investigation2011ISSN 0021-9738
Mercer, Jason et al.Vaccinia virus strains use distinct forms of macropinocytosis for host-cell entryProceedings of the National Academy of Sciences of the United States of America2010ISSN 0027-8424
Su, Shih Chi et al.Annexin 2 regulates endothelial morphogenesis by controlling AKT activation and junctional integrityJournal of Biological Chemistry2010ISSN 0021-9258
Castillo-Lluva, Sonia et al.SUMOylation of the GTPase Rac1 is required for optimal cell migrationNature Cell Biology2010ISSN 1465-7392
Duan, Lei et al.Distinct roles for Rho Versus Rac/Cdc42 GTPases downstream of Vav2 in regulating mammary epithelial acinar architectureJournal of Biological Chemistry2010ISSN 0021-9258
Thomas, Penny S. et al.Neural crest cell-specific deletion of Rac1 results in defective cell-matrix interactions and severe craniofacial and cardiovascular malformationsDevelopmental Biology2010ISSN 1095-564X
Ford-Speelman, Diana L. et al.The rho-guanine nucleotide exchange factor domain of obscurin activates rhoA signaling in skeletal muscleMolecular Biology of the Cell2009ISSN 1059-1524
Hsu, Hsiang Hao et al.Mechanisms of angiotensin II signaling on cytoskeleton of podocytesJournal of Molecular Medicine2008ISSN 0946-2716
Ivanov, Andrei I. et al.Myosin II regulates the shape of three-dimensional intestinal epithelial cystsJournal of Cell Science2008ISSN 0021-9533
Mitchell, Troy et al.Primary granule exocytosis in human neutrophils is regulated by Rac-dependent actin remodelingAmerican Journal of Physiology - Cell Physiology2008ISSN 1522-1563

Question 1:  What is the antigen that this antibody was raised against?

Answer 1:  The Rac1 specific antibody (Cat. # ARC03) is a mouse monoclonal antibody and it was raised against the C-terminal peptide of human Rac1.

 

Question 2:  What species has this antibody been tested against?

Answer 2:  The Rac1 specific antibody has been used to label Rac1 proteins in human, bovine, rat and mouse extracts.

 

If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com.