Anti-Cdc42: Mouse Mab

$0.00

SKU

ACD04

Datasheet

MSDS

Amount

Full Size: 1x500 µl (Cat. # ACD04) + $403.00

Trial Size: 1x125 µl (Cat. # ACD04-S) + $113.00

Qty:

Details

Introduction
Cdc42 (mol. wt. 21 kDa) belongs to the Rho-family of small G-proteins. The Rho family consists of at least 22 members, the most extensively characterized of which are the Rac1, RhoA and Cdc42 proteins. In common with all other small G-proteins, Rho family proteins act as molecular switches that transmit cellular signals through an array of effector proteins. The family mediates a diverse number of cellular responses including cytoskeletal reorganization, regulation of transcription, apoptosis and neuronal morphology.

Proteins within the Rho-family share 40-95% amino acid identity within their GTPase domains.

Advantages

> ACD04 has superior lot-to-lot consistency: all Cytoskeleton Inc. antibodies are QC'd for highly reproducible signal and detection.

> ACD04 is priced favorably compared to other Cdc42 antibodies (see table below)

Click Here for Detailed Methods

Material
The anti-Cdc42 antibody is a mouse monoclonal antibody. The antibody was raised against a peptide sequence of human Cdc42 (amino acids 150-182), it specifically recognizes Cdc42 in a wide range of species, including mammalian, reptile and avian and amphibian sources. Quality Control analysis has shown that the antibody does not recognize Rac1. ACD04 is supplied as a lyophilized white powder. Each Lot of antibody is quality controlled to provide a high batch to batch consistency.

Figure 1: Western Blot: Demonstration of ACD04 sensitivity & specificity

Cell extracts were separated by SDS-PAGE and transferred to a PVDF membrane according to the method given in the associated datasheet. Anti-Cdc42 was diluted 1 in 1000 in TBST and western analysis was performed as detailed in the Western Blot Method section. The following lysates were run on the gel: Lane 1: 20 μg A431 cells, Lane 2: 20 μg HeLa cells, Lane 3: 20 μg porcine brain, Lane 4: 500 μg His-tagged Rac1 protein (m.wt. 21 kD). The native Cdc42 band is visible at approximately 23 kD. Exposure time 2 minutes.

About

For product Datasheets and MSDSs please click on the PDF links below.

Sample Size Datasheet (Cat. ACD04-S):

Citations

If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com

Select list of citations of our previous Cdc42 antibody (ACD03). These references may be a useful tool, as ACD04 and ACD03 performed very similarly in our various QC applications.

Author	Title	Journal	Year	Article Link
Jayabal, Panneerselvam et al.	Roles of USP1 in Ewing sarcoma	Genes & Cancer	2024	PMID 38323120
Ledoux, Benjamin et al.	Plasma membrane nanodeformations promote actin polymerization through CIP4/CDC42 recruitment and regulate type II IFN signaling	Science Advances	2023	ISSN 2375-2548
Fu, Lisheng et al.	Septin11 promotes hepatocellular carcinoma cell motility by activating RhoA to regulate cytoskeleton and cell adhesion	Cell Death & Disease 2023 14:4	2023	ISSN 2041--4889
Serwe, Guillaume et al.	CNK2 promotes cancer cell motility by mediating ARF6 activation downstream of AXL signalling	Nature Communications	2023	ISSN 2041--1723
Kamata, Tamihiro et al.	Statins mediate anti- and pro-tumourigenic functions by remodelling the tumour microenvironment	DMM Disease Models and Mechanisms	2022	ISSN 1754-8411
Flores-Muñoz, Carolina et al.	The Long-Term Pannexin 1 Ablation Produces Structural and Functional Modifications in Hippocampal Neurons	Cells	2022	ISSN 2073-4409
Chen, Bo et al.	Transient neuroinflammation following surgery contributes to long-lasting cognitive decline in elderly rats via dysfunction of synaptic NMDA receptor	Journal of Neuroinflammation	2022	ISSN 1742-2094
Tyckaert, François et al.	Rac1, the actin cytoskeleton and microtubules are key players in clathrin-independent endophilin-A3-mediated endocytosis	Journal of cell science	2022	ISSN 1477-9137
Kamata, Tamihiro et al.	Statins mediate anti- and pro-tumourigenic functions by remodelling the tumour microenvironment	DMM Disease Models and Mechanisms	2022	ISSN 1754-8411
Li, Jingxuan et al.	Eva1a ameliorates atherosclerosis by promoting re-endothelialization of injured arteries via Rac1/Cdc42/Arpc1b	Cardiovascular Research	2021	ISSN 1755-3245
Barbera, Stefano et al.	The C-type lectin CD93 controls endothelial cell migration via activation of the Rho family of small GTPases	Matrix Biology	2021	ISSN 1569-1802
Jayabal, Panneerselvam et al.	NELL2-cdc42 signaling regulates BAF complexes and Ewing sarcoma cell growth	Cell Reports	2021	ISSN 2211-1247
Howden, Jake D. et al.	α2β1 integrins spatially restrict Cdc42 activity to stabilise adherens junctions	BMC biology	2021	ISSN 1741-7007
Hosseini, Kamran et al.	EMT-Induced Cell-Mechanical Changes Enhance Mitotic Rounding Strength	Advanced Science	2020	ISSN 2198-3844
Gorisse, Laetitia et al.	Ubiquitination of the scaffold protein IQGAP1 diminishes its interaction with and activation of the Rho GTPase CDC42	Journal of Biological Chemistry	2020	ISSN 1083-351X
Oni, Tobiloba E. et al.	SOAT1 promotes mevalonate pathway dependency in pancreatic cancer	Journal of Experimental Medicine	2020	ISSN 1540-9538
Gu, Jiawen et al.	Rho-GEF trio regulates osteoclast differentiation and function by Rac1/Cdc42	Experimental Cell Research	2020	ISSN 1090-2422
Singh, Rajesh K. et al.	Dynamic Actin Reorganization and Vav/Cdc42-Dependent Actin Polymerization Promote Macrophage Aggregated LDL (Low-Density Lipoprotein) Uptake and Catabolism	Arteriosclerosis, Thrombosis, and Vascular Biology	2019	ISSN 1524-4636
Huang, Yuxing et al.	Arp2/3-branched actin maintains an active pool of GTP-RhoA and controls RhoA abundance	Cells	2019	ISSN 2073-4409
Dupraz, Sebastian et al.	RhoA Controls Axon Extension Independent of Specification in the Developing Brain	Current Biology	2019	ISSN 0960-9822
Vestre, Katharina et al.	Rab6 regulates cell migration and invasion by recruiting Cdc42 and modulating its activity	Cellular and Molecular Life Sciences	2019	ISSN 1420-9071
MacKeil, Jodi L. et al.	Phosphodiesterase 3B (PDE3B) antagonizes the anti-angiogenic actions of PKA in human and murine endothelial cells	Cellular Signalling	2019	ISSN 1873-3913
Dudvarski Stanković, Nevenka et al.	EGFL7 enhances surface expression of integrin α 5 β 1 to promote angiogenesis in malignant brain tumors	EMBO Molecular Medicine	2018	ISSN 1757--4676
Wu, Nan et al.	RCC2 over-expression in tumor cells alters apoptosis and drug sensitivity by regulating Rac1 activation	BMC Cancer	2018	ISSN 1471-2407
Peretti, Amanda S. et al.	The R-Enantiomer of Keto***** Delays Mammary Tumor Development in Mouse Mammary Tumor Virus-Polyoma Middle T Antigen (MMTV-PyMT) Mice	American Journal of Pathology	2018	ISSN 1525-2191
Liu, Xiaolei et al.	Rasip1 controls lymphatic vessel lumen maintenance by regulating endothelial cell junctions	Development (Cambridge)	2018	ISSN 1477-9129
Lam, Jonathan G.T. et al.	Host cell perforation by listeriolysin O (LLO) activates a Ca2+-dependent cPKC/Rac1/Arp2/3 signaling pathway that promotes Listeria monocytogenes internalization independently of membrane resealing	Molecular Biology of the Cell	2018	ISSN 1939-4586
Li, Cao et al.	Regulation of staphylococcus aureus infection of macrophages by CD44, reactive oxygen species, and acid sphingomyelinase	Antioxidants and Redox Signaling	2018	ISSN 1557-7716
Ghézali, Grégory et al.	Connexin 30 controls astroglial polarization during postnatal brain development	Development (Cambridge)	2018	ISSN 1477-9129
Kawasaki, Natsumi et al.	TUFT1 interacts with RABGAP1 and regulates mTORC1 signaling	Cell Discovery	2018	ISSN 2056-5968
Stypulkowski, Ewa et al.	The depalmitoylase APT1 directs the asymmetric partitioning of Notch and Wnt signaling during cell division	Science Signaling	2018	ISSN 1937-9145
Vodicska, Barbara et al.	MISP regulates the IQGAP1/Cdc42 complex to collectively orchestrate spindle orientation and mitotic progression	Scientific Reports	2018	ISSN 2045-2322
Bucka, Kenneth B. et al.	Local Arp2/3-dependent actin assembly modulates applied traction force during apCAM adhesion site maturation	Molecular Biology of the Cell	2017	ISSN 1939-4586
Chang, Ting Ya et al.	Paxillin facilitates timely neurite initiation on soft-substrate environments by interacting with the endocytic machinery	eLife	2017	ISSN 2050-084X
Saito, Masaki et al.	Tctex‐1 controls ciliary resorption by regulating branched actin polymerization and endocytosis	EMBO reports	2017	ISSN 1469--221X
Takeuchi, Hiroki et al.	Intracellular periodontal pathogen exploits recycling pathway to exit from infected cells	Cellular Microbiology	2016	ISSN 1462-5822
Seidelin, Jakob Benedict et al.	Cellular inhibitor of apoptosis protein 2 controls human colonic epithelial restitution, migration, and Rac1 activation	American Journal of Physiology - Gastrointestinal and Liver Physiology	2015	ISSN 1522-1547
Thomas, Audrey et al.	Involvement of the Rac1-IRSp53-Wave2-Arp2/3 Signaling Pathway in HIV-1 Gag Particle Release in CD4 T Cells	Journal of Virology	2015	ISSN 0022--538X
Liu, Chunqiao et al.	Null and hypomorph Prickle1 alleles in mice phenocopy human Robinow syndrome and disrupt signaling downstream of Wnt5a	Biology Open	2014	ISSN 2046-6390
David, Muriel D. et al.	The RhoGAP ARHGAP19 controls cytokinesis and chromosome segregation in T lymphocytes	Journal of Cell Science	2014	ISSN 0021-9533
Valtcheva, Nadejda et al.	The orphan adhesion G protein-coupled receptor GPR97 regulates migration of lymphatic endothelial cells via the small GTPases RhoA and Cdc42	Journal of Biological Chemistry	2013	ISSN 0021-9258
Barrio, Laura et al.	TLR4 Signaling Shapes B Cell Dynamics via MyD88-Dependent Pathways and Rac GTPases	The Journal of Immunology	2013	ISSN 0022--1767
Steele, Brian M. et al.	WNT-3a modulates platelet function by regulating small GTPase activity	FEBS Letters	2012	ISSN 0014-5793
Mercer, Jason et al.	Vaccinia virus strains use distinct forms of macropinocytosis for host-cell entry	Proceedings of the National Academy of Sciences of the United States of America	2010	ISSN 0027-8424