Cdc42 protein: GSt tagged: human wid type

Cdc42 protein: GSt tagged: human wid type

Product Uses Include

  • Cdc42 biochemistry
  • Cdc42 GTPase assays
  • Cdc42 nucleotide exchange assays
  • Cdc42 binding studies

The human Cdc42 protein has been produced in a bacterial expression system. The protein is supplied as a lyophilized powder. When it is reconstituted in distilled water to 1 mg/ml, the protein is in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl2, 0.5% sucrose, 0.1% dextran. Protein concentration is determined by the Precision Red Advanced Protein Assay Reagent, Cat. # ADV02.

The recombinant protein is 50 kDa, consisting of the 22 kDa Cdc42 protein plus a 28 kDa GST tag in the amino-terminus.

For other forms of Cdc42 as well as many other purified small G-proteins, see our main small G-protein product page.

Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. GST-Cdc42 samples are >90% pure.

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Figure 1: GST-Cdc42 protein purity determination. A 20 µg sample of CDG01 (GST-Cdc42 molecular weight approx. 50 kDa) was separated by electrophoresis in a 12% SDS-PAGE system. The protein was stained with Coomassie Blue.

Biological Activity
Biological activity of the protein is determined by demonstrating that in GTP bound CDG01 associates with PAK protein while the GDP bound form of CDG01 does not associate with PAK protein in a pull down assay.

For product Datasheets and MSDSs please click on the PDF links below.   For additional information, click on the FAQs tab above or contact our Technical Support department at

AuthorTitleJournalYearArticle Link
Cheng, Zhi Jie et al.Co-regulation of caveolar and Cdc42-dependent fluid phase endocytosis by phosphocaveolin-1Journal of Biological Chemistry2010ISSN 0021-9258
Zhang, Xiao Feng et al.Rho-Dependent Contractile Responses in the Neuronal Growth Cone Are Independent of Classical Peripheral Retrograde Actin FlowNeuron2003ISSN 0896-6273
Lesser, C. F. et al.Expression of microbial virulence proteins in Saccharomyces cerevisiae models mammalian infectionThe EMBO Journal2001ISSN 1460--2075
Slater, S. J. et al.Interaction of Protein Kinase C Isozymes with Rho GTPases†Biochemistry2001ISSN 0006-2960


Question 1:  After reconstituting the lyophilized protein with water, what is the composition of the buffer the wild-type human Cdc42 protein is in?

Answer 1:  The protein should be reconstituted to 1 mg/ml by the addition of 25 μl of distilled water per tube.  The protein will then be in the following buffer: 10 mM Tris pH 7.5, 20 mM NaCl, 0.3 mM MgCl2, 1.0% sucrose, and 0.2% dextran.  In order to maintain high biological activity of the protein, it is strongly recommended that the protein solution be supplemented with DTT to 1 mM final concentration


Question 2:  What is the recommended way to store the wild-type Cdc42 protein to maintain high activity?

Answer 2:  In order to maintain high biological activity of the protein, it is strongly recommended that the protein solution be supplemented with DTT to 1 mM final concentration, aliquoted into experiment sized amounts (e.g. 10 μg per aliquot), snap frozen in liquid nitrogen, and stored at -70°C. The protein is stable for 6 months if stored at -70°C.  The protein should not be exposed to repeated freeze-thaw cycles.  The lyophilized protein is stable for 1 year if stored desiccated to <10% humidity at 4°C.



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