Product Uses Include
- Test binding activity of known or proteins or compounds to tubulin or microtubules.
- Test polymerization in the presence of microtubule / tubulin binding proteins.
- Test conditions that may perturb MAP binding.
Introduction
This kit is intended for those researchers who are not accustomed to working with tubulin protein in vitro. The contents of this kit will allow you to reproducibly prepare microtubules of a predetermined mean length. After polymerization the microtubules can be used directly or stabilized with taxol before use, depending upon the needs of your experimental system.
Kit contents
The kit contains enough materials for 8-200 assays depending on experimental setup. The following components are included:
- 8 x 250 µg Tubulin, >99% pure (Cat. # TL238)
- General Tubulin Buffer (Cat. # BST01)
- GTP (Cat. # BST06)
- Microtubule glycerol Buffer (Cat. # BST05)
- Taxol Stock Solution (Cat. # TXD01)
- Dry DMSO
- Manual with detailed protocols and extensive troubleshooting guide
For product Datasheets and MSDSs please click on the PDF links below. For additional information, click on the FAQs tab above or contact our Technical Support department at tservice@cytoskeleton.com
Product Description
MSDS
Datasheet
Cat #
Microtubules/Tubulin Biochem Kit
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BK015
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van Horck, F. P., Ahmadian, M. R., Haeusler, L. C., Moolenaar, W. H. and Kranenburg, O. (2001). Characterization of p190RhoGEF, a RhoA-specific guanine nucleotide exchange factor that interacts with microtubules. J. Biol. Chem. 276, 4948-4956.
Coming soon! If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com
