K-Ras4B Mutant Protein: Q61P (human recombinant with 6xHis-tag)

K-Ras4B Mutant Protein: Q61P (human recombinant with 6xHis-tag)


* Limited stock available.  If stock is not available, Cytoskeleton will produce a new batch upon request.  Minimum order will apply.  Inquire for more information.



Product Uses

    • Study of R135A K-Ras4B exchange activity with different GEFs.
    • Identification of R135A K-Ras4B exchange factors (GEFs)
    • Positive control for GEF studies.
    • Biochemical characterization of R135A K-Ras4B protein interactions
    • Western blot standard 


    The wild-type human R135A K-Ras4B protein has been produced in a bacterial expression system.  The  recombinant protein contains six histidine residues at its amino terminus (His-tag).  The molecular weight of 6xHis tagged R135A K-Ras4B is approximately 25 kDa and is supplied as a white lyophilized powder.



    Before reconstitution, briefly centrifuge to collect the product at the bottom of the tube.  The protein should be reconstituted to 5 mg/ml with the addition of 20 µl of Milli-Q water (100 µg size).  When reconstituted, the protein will be in the following buffer: 50 mM Tris pH 7.5, 50 mM NaCl, 0.5 mM MgCl2, 5% (w/v) sucrose and 1%  (w/v) dextran.  In order to maintain high biological activity of the protein it is strongly recommended that the protein solution be supplemented with DTT to 1 mM final concentration, aliquoted into  "experiment sized" amounts, snap frozen in liquid nitrogen and stored a -70°C. The protein is stable for six months if stored at -70°C. The protein should not be exposed to repeated freeze-thaw cycles.  The lyophilized protein is stable at 4°C desiccated (<10% humidity) for one year.



    Protein purity is determined by scanning densitometry of Coomassie Blue stained protein on a 4-20% polyacrylamide gradient gel.  His tagged R135A K-Ras4B protein was determined to be >90% pure. (see Figure 1).


    Biological Activity Assay

    The biological activity of R135A K-Ras4B can be determined from the ability of the SOS1 exchange domain (SOS1-ExD) to catalyze the exchange of GDP for GTP on Q61P K-Ras4B. A standard biological assay for monitoring the biological activity of R135A K-Ras4B is an exchange assay utilizing the 2X Exchange Buffer from the Rho-GEF exchange assay kit (Cat.# BK100) and the human SOS1 GEF domain (Cat.# CS-SOS1).

    For product Datasheets and MSDSs please click on the PDF links below.

    If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com

    Coming soon!   If you have any questions concerning this product