BlastR Rapid Filtration Kit

The BlastR™ filters allows for fast and reliable preparation of genomic DNA (gDNA)-free cell lysate for western blot or immunoprecipitation when used in combination with a denaturing buffer, such as guanidine, urea or high SDS based extraction buffers that usually produce copious viscosity.  gDNA contamination is a significant problem with denaturing buffers that can interfere with downstream applications like immunoprecipitation and migration of proteins in SDS-PAGE. Unlike sonication or insulin needle gDNA shearing methods, the BlastR™ filter effectively removes gDNA contamination while having no effect on the integrity of the proteins in the lysate. 

Product Uses Include 

• Reduce viscosity of samples 
• Rapid isolation of protein extracts
• Preparation of extracts from denaturing lysis buffers 

Validation Data: BlastR Rapid Filtration Kit White Paper

Sample Kit Components (50 filters)

 BlastR Filter set 50 units.

 

Clarification of gDNA from BlastR™ lysate (A) Viscous sample lysate loaded onto BlastR™ Filter. (B) Sample passed through Filter system where gDNA is captured.  >90% recovery of protein in cell lysate.

 

For the most recent publications citing this and other Signal-Seeker™ products, see our Signal-Seeker™ Validation Data Page click here