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BlastR Rapid Filtration Kit
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The BlastR™ filters allows for fast and reliable preparation of genomic DNA (gDNA)-free cell lysate for western blot or immunoprecipitation when used in combination with a denaturing buffer, such as guanidine, urea or high SDS based extraction buffers that usually produce copious viscosity. gDNA contamination is a significant problem with denaturing buffers that can interfere with downstream applications like immunoprecipitation and migration of proteins in SDS-PAGE. Unlike sonication or insulin needle gDNA shearing methods, the BlastR™ filter effectively removes gDNA contamination while having no effect on the integrity of the proteins in the lysate.
Product Uses Include
- Reduce viscosity of samples
- Rapid isolation of protein extracts
- Preparation of extracts from denaturing lysis buffers
Validation Data: BlastR Rapid Filtration Kit White Paper
Sample Kit Components (50 filters)
Clarification of gDNA from BlastR™ lysate (A) Viscous sample lysate loaded onto BlastR™ Filter. (B) Sample passed through Filter system where gDNA is captured. >90% recovery of protein in cell lysate.
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For the most recent publications citing this and other Signal-Seeker™ products, see our Signal-Seeker™ Validation Data Page click here
Visit our Signal-Seeker™ Tech Tips and FAQs page for technical tips and frequently asked questions regarding this and other Signal-Seeker™ products click here
If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com
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