As part of the Signal-Seeker™ product line, SUMOylation 2/3 affinity beads have been optimized in order to detect endogenous levels of SUMO 2/3 modified proteins, which often represent <1% of the target protein. SUMOylation 2/3 affinity beads comprise an anti-SUMO-2/3 antibody (Clone 11G2) that has been chemically conjugated to Protein G beads. Validation studies done in Hela cells have shown that these beads can immunoprecipitate a wide range of SUMO 2/3 modified proteins without detectable leaching of either heavy or light chains in an IP assay. A comprehensive Signal-Seeker™ SUMOylation 2/3 Detection Kit is also available (BK162) and is recommended for first time users.
Immunoprecipitation using the Signal-Seeker™ SUMOylation 2/3 Detection Kit
Denatured cell lysates were prepared as previously reported1 from HeLa cells ("HS43": Heat Shock treated 42°C for 10 min, and "CT37": untreated) and HeLa siRNA SUMO knockdown ("KDS2"). 1mg of lysate was used for the immunoprecipitation (IP) of SUMOylated 2/3 proteins. Western blots of immunoprecipitated proteins were developed using anti-SUMO-2/3 antibody (Cytoskeleton cat# ASM23) (A) or anti-Ubc9 antibody (B). The level of SUMO-2/3 conjugates in heat shock treated cells is higher than control, and shRNA SUMO-2 knock-down reduced the level of SUMOylated 2/3 modified proteins. Chemical conjugation of SUMO-2/3 antibody (11G2) to the affinity bead matrix prevents heavy and light chain leaching. Unconjugated free SUMO is also captured by the SUMO-2/3 affinity beads.
(B) Unmodified Ubc9 is visible near 18kDa. High molecular-weight band indicates that Ubc9 is SUMOylated by a single SUMO-2/3 protein. Ubc9 has previously been reported to be a target for SUMOylation1,2.
1. Barysch S. et al. 2014. Identification and analysis of endogenous SUMO1 and SUMO2/3 targets in mammalian cells and tissues using monoclonal antibodies. Nat Protoc. 9(4):896-909
2. Becker J. et al. 2013. Detecting endogenous SUMO targets in mammalian cells and tissues. Nature Struc. & Mol. Biol. 20, 525-531.
Each package contains enough SUMOylation 2/3 beads for 20 reactions.
For more information contact: email@example.com
Signal-Seeker™ SUMOylation 2/3 Detection Kit (Cat. # BK162)
Signal-Seeker™ Ubiquitination Detection Kit (Cat. # BK161)
Signal-Seeker™ Phosphotyrosine Detection Kit (Cat. # BK160)
Signal-Seeker™: BlastR™ Rapid Lysate Prep Kit (Cat. # BLR01)
Signal-Seeker™: PTMtrue™ SUMOylation 2/3 Antibody (Cat.# ASM23)
|Waters, Emily et al.||The SUMO protease SENP3 regulates mitochondrial autophagy mediated by Fis1||EMBO reports||2022||ISSN 1469--221X|
|Zhou, Liwen et al.||SUMOylation stabilizes hSSB1 and enhances the recruitment of NBS1 to DNA damage sites||Signal Transduction and Targeted Therapy||2020||ISSN 2059-3635|
|Horita, Henrick et al.||Utilizing a comprehensive immunoprecipitation enrichment system to identify an endogenous post-translational modification profile for target proteins||Journal of Visualized Experiments||2018||ISSN 1940-087X|
|Horita, Henrick et al.||Utilizing optimized tools to investigate PTM crosstalk: Identifying potential PTM crosstalk of acetylated mitochondrial proteins||Proteomes||2018||ISSN 2227-7382|
|Horita, Henrick et al.||A simple toolset to identify endogenous post-translational modifications for a target protein: A snapshot of the EGFR signaling pathway||Bioscience Reports||2017||ISSN 1573-4935|
|Horita, Henrick et al.||Identifying Regulatory Posttranslational Modifications of PD-L1: A Focus on Monoubiquitinaton||Neoplasia (United States)||2017||ISSN 1476-5586|