Proprietary labeling yields 0.2–0.7 dyes/heterodimer for consistent, active tubulin. Abs/Em: 650/670 nm. Useful for in vitro microtubule, tubulin, and kinesin motility assays.

• Fluorescent microtubule substrates for in vitro kinesin motility assays
• Microtubule dynamics studies in vitro
• Analyze effects of MAPs & drugs on microtubule dynamics in vitro

Tubulin protein T240 is labeled on random surface lysines using HiLyte™647 activated ester;

• Ex: 649 nm, Em:674 nm – far-red spectrum
• Labeling stoichiometry: ~0.1-0.3 dyes per tubulin heterodimer
• Supplied as a lyophilized powder

Protein purity is assessed by scanning densitometry of Coomassie Blue stained protein on a12% polyacrylamide gel. Purity is determined to be >99% pure.

TL670M tubulin retains high biological activity, with >80% polymerizing into microtubules in vitro, as confirmed by a spin-down assay. This performance is comparable to that of unmodified tubulin.