• Fluorescent microtubule substrates for in vitro kinesin motility assays
• Microtubule dynamics studies in vitro
• Analyze effects of MAPs & drugs on microtubule dynamics in vitro

Tubulin protein T240 is labeled on random surface lysines using an activated ester of rhodamine;

• Ex: 547 nm, Em:576 nm – orange spectrum
• Labeling stoichiometry: ~1–2 dyes per tubulin heterodimer
• Supplied as a lyophilized powder

Protein purity is assessed by scanning densitometry of Coomassie Blue stained protein on a12% polyacrylamide gel. Purity is determined to be >99% pure.

TL590M tubulin retains high biological activity, with >80% polymerizing into microtubules in vitro, as confirmed by a spin-down assay. This performance is comparable to that of unmodified tubulin.