General tubulin buffer: 1x stock

General tubulin buffer: 1x stock
$0.00

Product Uses Include

  •  Resuspension and storage of tubulin

Material
General tubulin buffer (PEM). Contains 80 mM PIPES pH 6.9, 2 mM MgCl2 and 0.5 mM EGTA. Used as a tubulin working buffer in the form of G-PEM (i.e. PEM plus 1 mM GTP (Cat.# BST06)) or a tubulin polymerization buffer in the form of glycerol containing G-PEM (PEM plus 1 mM GTP (Cat. # BST06) and e.g. 5% glycerol (see Cat. # BST05)).

For product Datasheets and MSDSs please click on the PDF links below.   For additional information, click on the FAQs tab above or contact our Technical Support department at tservice@cytoskeleton.com

AuthorTitleJournalYearArticle Link
Singh, Rajendra K. et al.Coordinated Biophysical Stimulation of MSCs via Electromagnetized Au-Nanofiber Matrix Regulates Cytoskeletal-to-Nuclear Mechanoresponses and Lineage SpecificationAdvanced Functional Materials2023ISSN 1616--3028
Feizabadi, Mitra Shojania et al.The Effect of Tau and Taxol on Polymerization of MCF7 Microtubules In VitroInternational Journal of Molecular Sciences2022ISSN 1422-0067
Wu, R. et al.Thermal hysteresis in microtubule assembly/disassembly dynamics: The aging-induced degradation of tubulin dimersBiochemistry and Biophysics Reports2022ISSN 2405-5808
Pizzi, Rita et al.Evidences of new biophysical properties of microtubulesFocus on Artificial Neural Networks2021Article Link
Hough, Cameron M. et al.Disassembly of microtubules by intense terahertz pulsesBiomedical Optics Express2021ISSN 2156--7085
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Question 1:  How does general tubulin buffer differ from G-PEM buffer?

Answer 1: Basically, these buffers are the same except that G-PEM contains 1mM GTP to support tubulin polymerization.  General tubulin buffer (Cat. # BST01) and PEM buffer typically contain 80 mM PIPES, 2 mM MgCl2, 0.5 mM EGTA pH 7.0.  Both buffers should be supplemented with 1 mM GTP (hence, the “G” in G-PEM buffer).

 

Question 2: Is glycerol necessary for the maintenance of biologically-active tubulin?

Answer 2: Glycerol is often added to a final concentration of 5 - 10% to enhance polymerization; however, glycerol is not necessary for the maintenance of biologically active tubulin.

 

 

If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com