General tubulin buffer: 1x stock

$0.00

SKU

BST01

Datasheet

MSDS

Amount

1x10 ml (Cat. # BST01-001) + $77.00

1x100 ml (Cat. # BST01-010) + $171.00

Qty:

Details

Product Uses Include

Resuspension and storage of tubulin

Material
General tubulin buffer (PEM). Contains 80 mM PIPES pH 6.9, 2 mM MgCl₂ and 0.5 mM EGTA. Used as a tubulin working buffer in the form of G-PEM (i.e. PEM plus 1 mM GTP (Cat.# BST06)) or a tubulin polymerization buffer in the form of glycerol containing G-PEM (PEM plus 1 mM GTP (Cat. # BST06) and e.g. 5% glycerol (see Cat. # BST05)).

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About

For product Datasheets and MSDSs please click on the PDF links below. For additional information, click on the FAQs tab above or contact our Technical Support department at tservice@cytoskeleton.com

Citations

Author	Title	Journal	Year	Article Link
Singh, Sudhir K. et al.	Noncanonical interaction with microtubules via the N-terminal nonmotor domain is critical for the functions of a bidirectional kinesin	Science Advances	2024	ISSN 2375-2548
Hammarin, Greger et al.	No observable non-thermal effect of microwave radiation on the growth of microtubules	Scientific Reports	2024	ISSN 2045--2322
Singh, Rajendra K. et al.	Coordinated Biophysical Stimulation of MSCs via Electromagnetized Au-Nanofiber Matrix Regulates Cytoskeletal-to-Nuclear Mechanoresponses and Lineage Specification	Advanced Functional Materials	2023	ISSN 1616--3028
Feizabadi, Mitra Shojania et al.	The Effect of Tau and Taxol on Polymerization of MCF7 Microtubules In Vitro	International Journal of Molecular Sciences	2022	ISSN 1422-0067
Wu, R. et al.	Thermal hysteresis in microtubule assembly/disassembly dynamics: The aging-induced degradation of tubulin dimers	Biochemistry and Biophysics Reports	2022	ISSN 2405-5808
Pizzi, Rita et al.	Evidences of new biophysical properties of microtubules	Focus on Artificial Neural Networks	2021	Article Link
Hough, Cameron M. et al.	Disassembly of microtubules by intense terahertz pulses	Biomedical Optics Express	2021	ISSN 2156--7085
Li, Yaxin et al.	Small-Molecule HSP27 Inhibitor Abolishes Androgen Receptors in Glioblastoma	Journal of Medicinal Chemistry	2021	ISSN 1520-4804
Lin, Jong Wei et al.	Dexamethasone accelerates muscle regeneration by modulating kinesin-1-mediated focal adhesion signals	Cell Death Discovery	2021	ISSN 2058-7716
McElmurry, Kristi et al.	Dynein-mediated microtubule translocation powering neurite outgrowth in chick and Aplysia neurons requires microtubule assembly	Journal of Cell Science	2020	ISSN 1477-9137
Tuszynski, Jack A. et al.	Microtubules as Sub-Cellular Memristors	Scientific Reports	2020	ISSN 2045-2322
Kalra, Aarat P. et al.	Behavior of α, β tubulin in DMSO-containing electrolytes	Nanoscale Advances	2019	ISSN 2516-0230
Melo, Esther et al.	HtrA1 Mediated Intracellular Effects on Tubulin Using a Polarized RPE Disease Model	EBioMedicine	2018	ISSN 2352-3964
Chen, Yang et al.	Visualizing Autophagic Lysosome Reformation in Cells Using In Vitro Reconstitution Systems	Current Protocols in Cell Biology	2018	ISSN 1934-2616
Lukinavičius, Gražvydas et al.	Fluorescent dyes and probes for super-resolution microscopy of microtubules and tracheoles in living cells and tissues	Chemical Science	2018	ISSN 2041-6539
Mangiatordi, Giuseppe Felice et al.	Automated identification of structurally heterogeneous and patentable antiproliferative hits as potential tubulin inhibitors	Chemical Biology and Drug Design	2018	ISSN 1747-0285
Santelices, Iara B. et al.	Response to Alternating Electric Fields of Tubulin Dimers and Microtubule Ensembles in Electrolytic Solutions	Scientific Reports	2017	ISSN 2045-2322
Tantak, Mukund P. et al.	Design and synthesis of bis(indolyl)ketohydrazide-hydrazones: Identification of potent and selective novel tubulin inhibitors	European Journal of Medicinal Chemistry	2017	ISSN 1768-3254
Mangiatordi, Giuseppe Felice et al.	Novel chemotypes targeting tubulin at the colch***** binding site and unbiasing P-glycoprotein	European Journal of Medicinal Chemistry	2017	ISSN 1768-3254
DeBruhl, Heather et al.	Rop, the Sec1/Munc18 homolog in Drosophila, is required for furrow ingression and stable cell shape during cytokinesis	Journal of Cell Science	2016	ISSN 1477-9137
Lee, Dongmin et al.	Enhanced expression and purification of inositol 1,4,5-trisphosphate 3-kinase A through use of the pCold1-GST vector and a C-terminal hexahistidine tag in Escherichia coli	Protein Expression and Purification	2014	ISSN 1046-5928
Hartley, R. M. et al.	Polygamain, a New Microtubule Depolymerizing Agent That Occupies a Unique Pharmacophore in the Colch***** Site	Molecular Pharmacology	2012	ISSN 0026-895X
Chen, Chun Han et al.	Protopine, a novel microtubule-stabilizing agent, causes mitotic arrest and apoptotic cell death in human hormone-refractory prostate cancer cell lines	Cancer Letters	2012	PMID 22033245
Carletti, B. et al.	Effect of protein glutathionylation on neuronal cytoskeleton: a potential link to neurodegeneration	Neuroscience	2011	ISSN 1873--7544
Risinger, A. L. et al.	ELR510444, a novel microtubule disruptor with multiple mechanisms of action	The Journal of pharmacology and experimental therapeutics	2011	ISSN 1521--0103
Faridi, Uzma et al.	Proteomics indicates modulation of tubulin polymerization by L-menthol inhibiting human epithelial colorectal adenocarcinoma cell proliferation	Proteomics	2011	ISSN 1615--9861
Chang, Chia Hao et al.	Mycotoxin citrinin induced cell cycle G2/M arrest and numerical chromosomal aberration associated with disruption of microtubule formation in human cells	Toxicological sciences : an official journal of the Society of Toxicology	2011	ISSN 1096--0929
Ratnikova, Tatsiana A. et al.	In vitro polymerization of microtubules with a fullerene derivative	ACS Nano	2011	ISSN 1936-0851
Kawaratani, Yasuyuki et al.	New microtubule polymerization inhibitors comprising a nitrooxymethylphenyl group	Bioorganic and Medicinal Chemistry	2011	ISSN 0968-0896
O'Boyle, Niamh M. et al.	Synthesis and evaluation of azetidinone analogues of combretastatin A-4 as tubulin targeting agents	Journal of Medicinal Chemistry	2010	ISSN 0022-2623
Gutiérrez-Medina, Braulio et al.	Visualizing individual microtubules by bright field microscopy	American Journal of Physics	2010	ISSN 0002--9505
Davis, Ashley et al.	A tubulin polymerization microassay used to compare ligand efficacy	Methods in Cell Biology	2010	ISSN 0091-679X
Kushkuley, Jacob et al.	Neurofilament cross-bridging competes with kinesin-dependent association of neurofilaments with microtubules	Journal of cell science	2009	ISSN 1477--9137
Prager-Khoutorsky, Masha et al.	Allicin inhibits cell polarization, migration and division via its direct effect on microtubules	Cell Motility and the Cytoskeleton	2007	ISSN 0886-1544
Huang, Yao Ting et al.	CIL-102 interacts with microtubule polymerization and causes mitotic arrest following apoptosis in the human prostate cancer PC-3 cell line	The Journal of biological chemistry	2005	ISSN 0021--9258
Chen, Zehan et al.	A-432411, a novel indolinone compound that disrupts spindle pole formation and inhibits human cancer cell growth		2005	Article Link

Faqs

Question 1: How does general tubulin buffer differ from G-PEM buffer?

Answer 1: Basically, these buffers are the same except that G-PEM contains 1mM GTP to support tubulin polymerization. General tubulin buffer (Cat. # BST01) and PEM buffer typically contain 80 mM PIPES, 2 mM MgCl2, 0.5 mM EGTA pH 7.0. Both buffers should be supplemented with 1 mM GTP (hence, the “G” in G-PEM buffer).

Question 2: Is glycerol necessary for the maintenance of biologically-active tubulin?

Answer 2: Glycerol is often added to a final concentration of 5 - 10% to enhance polymerization; however, glycerol is not necessary for the maintenance of biologically active tubulin.

If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com