Anti-alpha/beta tubulin: sheep polyclonal

Anti-alpha/beta tubulin: sheep polyclonal
$0.00

Product Uses
This antibody is recommended for detection of tubulin in all yeast, fungi, and animal species (Fig. 2).
The following protocols have been tested with this antibody:

Western BlotImmunofluorescence
YesYes

RRID

AB_10708807

Material
The anti-tubulin antibody (Cat. # ATN02) is an affinity purified sheep polyclonal antibody that reacts to alpha and beta tubulin. The immunogen used for antibody production was a mixture of purified tubulins and therefore the antibody has broad species cross reactivity from yeast to humans. Porcine extract (Cat. # EXT03) is included as a positive control and ATN02 identifies a characteristic tubulin band at 55 kD on Western blots (see Fig. 1).  ATN02 is supplied as a lyophilized white powder.

 

Example Results


Western Blot of AAN01

Figure 1. Western blot analysis of anti-tubulin antibody.  Protein samples were separated by electrophoresis and transferred to PVDF membrane as described in the methods.  ATN02 antibody was diluted to 50 0 ng/ml (1:1000) for Western blot analysis.  Tubulin was detected in 10 µg of bovine brain extract (see arrow at 55 kD).  Molecular weight markers are from Invitrogen. 

Western Blot of ATN02

Figure 2. Western blot of cell extracts probed with anti-tubulin polyclonal antibody (Cat. # ATN02). Chemiluminescence detection of tubulin in extracts of Drosophila S2 cells (50 µg, lane 1) Xenopus A6 cells (50 µg, lane 2), mouse Swiss 3T3 cells (50 µg, lane 3), rat NRK cells (50 µg, lane 4), human HeLa cells (50 µg, lane 5), and bovine brain (50 µg, lane 6) at 55 kD (see arrow).  The blot was probed with a 500 ng/ml (1:1000) dilution of ATN02.

IF of AAN01

Figure 3. Immunofluorescence image of mouse Swiss 3T3 cells stained with Anti-Tubulin  polyclonal antibody (Cat. # ATN02). Swiss 3T3 cells were grown to semi-confluency and fixed with methanol. Immunofluorescence staining using 2.5 µg/ml (1:200 dilution) ATN02 antibody is shown (red). The primary antibody was detected with a 1:500 dilution of anti-sheep rhodamine conjugated antibody. DNA (blue) was stained with 100 nM DAPI in PBS. Photograph was taken with a 100X objective lens.

Immunofluorescence image using ATN02

Figure 4. Immunofluorescence image of Arabidopsis thaliana metaphase cell stained with Anti-Tubulin polyclonal antibody (Cat. # ATN02).
Microtubules are pseudocolored in green and chrmosomes in red.  

Image courtesy of Dr. Bo Liu, Department of Plant Biology, University of California, Davis.

For product Datasheets and MSDSs please click on the PDF links below.   For additional information, click on the FAQs tab above or contact our Technical Support department at tservice@cytoskeleton.com

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Question 1: What is the antigen that this antibody was raised against?

Answer 1:  The immunogen used for antibody production was a mixture of purified tubulins and therefore the polyclonal antibody has broad species cross reactivity from yeast to humans.

 

Question 2:  What species has this antibody been tested against?

Answer 2:  This polyclonal antibody is recommended for detection of tubulin in all yeast, fungi, and animal species including Drosophila melanogaster, Xenopus laevis, mouse, rat, human and bovine cell/tissue extracts. 

 

 

If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com