Validated for:

• Western blot-WB
• Immunofluorescence-IF

Anti-tubulin antibody (ATN02) was raised against a mixture of purified tubulins from a variety of species. It detects α and β tubulin isotypes (~55 kDa). The polyclonal Ab is purified on a tubulin affinity column.

Host/isotype:

• Sheep
• Polyclonal IgG

Reactivity:

• Broad cross species reactivity from yeast to human

Amount of material:

• ATN02: 2 x 100 µl when reconstituted (20-40 µg per tube)
• ATN02-S: 1 x 25 µl when reconstituted (5-10 µg per tube)

Working concentrations:

• WB 1:1000 dilution 0.2–0.4 µg/ml final 200 ml working volume - full size Ab
• IF 1:500 dilution 0.4-0.8 µg/ml final 100 ml working volume - full size Ab

Formulation

• Supplied as a lyophilized powder
• Buffer composition after reconstitution in 30% glycerol in water: 50 mM PIPES pH7.0, 5% sucrose, 1% dextran, 30% glycerol

Sensitivity:

• Detects as little as 10 ng purified porcine brain tubulin.
• In native samples, a 55 kD actin band is clearly visible in 0.5 µg porcine brain lysate

Specificity:

• Across a panel of cell lysates (including Drosophila, Xenopus, and human), Western blotting revealed a single band at ~55 kDa, corresponding to α- and β-tubulin.
• Under the recommended fixation protocols (see data sheet), microtubule structures are clearly revealed, with minimal to no staining of other cellular structures.