Product Uses Include
Guanosine triphosphate (GTP). 100 mM solution of GTP. Provided as a lyophilized powder. Required for tubulin stability and microtubule assembly and dynamics.
Chen et al., 2012. Protopine, a novel microtubule-stabilizing agent, causes mitotic arrest and apoptotic cell death in human hormone-refractory prostate cancer cell lines. Cancer Lett. 315, 1-11.
Hartley et al., 2012. Polygamain, a New Microtubule Depolymerizing Agent That Occupies a Unique Pharmacophore in the Colchicine Site. Mol. Pharmacol. 81, 431-439.
Chang et al., 2011. Mycotoxin Citrinin Induced Cell Cycle G2/M Arrest and Numerical Chromosomal Aberration Associated with Disruption of Microtubule Formation in Human Cells. Toxicol. Sci. 119, 84–92.
Risinger et al., 2011. ELR510444, A Novel Microtubule Disruptor with Multiple Mechanisms of Action. J. Pharmacol. Exp. Ther. 336, 652–660.
Faridi et al., 2011. Proteomics indicates modulation of tubulin polymerization by L-menthol inhibiting human epithelial colorectal adenocarcinoma cell proliferation. Proteomics. 11, 2115-2119.
Carletti et al., 2011. Effect of protein glutathionylation on neuronal cytoskeleton: a potential link to neurodegeneration. Neuroscience. 192, 285-294.
O'Boyle et al., 2010. Synthesis and Evaluation of Azetidinone Analogues of Combretastatin A-4 as Tubulin Targeting Agents. J. Med. Chem. 53, 8569-8584.
Kushkuley et al., 2009. Neurofilament cross-bridging competes with kinesin-dependent association of neurofilaments with microtubules. J Cell Sci. 122, 3579-86.
Chen et al., 2005. A-432411, a novel indolinone compound that disrupts spindle pole formation and inhibits human cancer cell growth. Mol. Cancer Ther. 4, 562-568.
Huang et al., 2005. CIL-102 interacts with microtubule polymerization and causes mitotic arrest following apoptosis in the human prostate cancer PC-3 cell line. J. Biol. Chem. 280, 2771-2779.
Rouzier et al., 2005. Microtubule-associated protein tau: A marker of paclitaxel sensitivity in breast cancer. Proc. Natl. Acad. Sci. U.S.A. 102, 8315-8320.
Jiang et al., 2002. Double blockade of cell cycle at G1-S transition and M phase by 3-iodoacetamido benzoyl ethyl ester, a new type of tubulin ligand. Cancer Res. 62, 6080-6088.
Mooberry et al., 1999. Laulimalide and isolaulimalide, new paclitaxel-like microtubule-stabilizing agents. Cancer Res. 59, 653-660.
Question 1: Does GTP need to be included in tubulin buffers prepared for reconstituting and storing tubulin if the tubulin will not be used for polymerization?
Answer 1: Cytoskeleton’s tubulin proteins are lyophilized in a buffer that contains 1 mM GTP, so omitting additional GTP (Cat. # BST06) in the reconstitution buffer can be done if the tubulin will not be used for polymerization. However, this is not recommended. Under most preparation conditions, GTP is required for tubulin stability and for the formation of microtubules in vivoand in vitro and we recommend adding fresh GTP (1 mM) to the PEM buffer used for reconstitution and polymerization.
Question 2: How long is GTP-containing tubulin buffer stable for when stored on ice the day of the experiment?
Answer 2:Buffers that contain GTP (Cat. # BST06) should not be kept longer than 2 hours on ice. After this time, GTP-supplemented buffers should be discarded. Do not attempt to freeze and re-use these buffers.
If you have any questions concerning this product, please contact our Technical Service department at email@example.com