Rhodamine Phalloidin

Rhodamine Phalloidin
$0.00

Product Uses Include

  • Stain F-actin in fixed cells
  • Stabilize actin filaments in vitro
  • Visualize actin filaments in vitro

Material
Phalloidin's usefulness as a laboratory tool is well established and lies in it's ability to inhibit microfilament de-polymerization. Thus, phalloidin stabilized microfilaments are used as substrates for the identification and characterization of the ever increasing number of microfilament associated proteins. In addition, rhodamine phalloidin can be used for staining the actin cytoskeleton in cells, and also as a fluorescent marker/stabilizer for microfilaments used in motility assays.

The rhodamine phalloidin is provided as a lyophilized powder. When reconstituted with 500 µl methanol, it is at 14 µM (200 x stock).

Purity
In keeping with our policy of providing only the highest quality products, we are now pleased to inform you that Cytoskeleton is offering rhodamine phalloidin at a chromatographic purity of >99%. This is the purest phalloidin commercially available for research use.

Biological Activity
We have determined that the microfilament stabilizing property of our product is equal or superior to any other commercially available phalloidins. Microfilaments in 70 nM phalloidin are stable at room temperature for over one week.

phdr1

Figure 1. A Swiss 3T3 fibroblast stained with rhodamine phalloidin (PHDR1). The phalloidin binds specifically to F-actin and does therefore stain the actin stress fibers in the cell

For product Datasheets and MSDSs please click on the PDF links below.   For additional information, click on the FAQs tab above or contact our Technical Support department at tservice@cytoskeleton.com

AuthorTitleJournalYearArticle Link
Skillin, Nathaniel P. et al.Stiffness anisotropy coordinates supracellular contractility driving long-range myotube-ECM alignmentScience Advances2024
Martin, Mary Ann et al.The Wolbachia WalE1 effector alters Drosophila endocytosisPLOS Pathogens2024
Hua, Wei et al.CD36-mediated podocyte lipotoxicity promotes foot process effacementOpen Medicine (Poland)2024
Oliveira, Ana et al.COX-2 inhibitor delivery system aiming intestinal inflammatory disordersBiomaterials Advances2024
Mu, Ning et al.Terahertz meta-biosensor for subtype detection and chemotherapy monitoring of glioma cellsMaterials & Design2024
Shi, Weili et al.Three-Dimensional Printed Silk Fibroin/Hyaluronic Acid Scaffold with Functionalized Modification Results in Excellent Mechanical Strength and Efficient Endogenous Cell Recruitment for Articular Cartilage RegenerationInternational Journal of Molecular Sciences2024
An, Junyan et al.Dual aldehyde cross-linked hyaluronic acid hydrogels loaded with PRP and NGF biofunctionalized PEEK interfaces to enhance osteogenesis and vascularizationMaterials Today Bio2024
Parekh, Mohit et al.Enhanced Migration of Fuchs Corneal Endothelial Cells by Rho Kinase Inhibition: A Novel Ex Vivo Descemet’s Stripping Only ModelCells2024
Motizuki, Mitsuyoshi et al.The Snail signaling branch downstream of the TGF-β/Smad3 pathway mediates Rho activation and subsequent stress fiber formationJournal of Biological Chemistry2024
Zhang, Yi-Ding et al.Nanoclay-Modified Hyaluronic Acid Microspheres for Bone Induction by Sustained rhBMP-2 DeliveryMacromolecular Bioscience2024
Kong, Lingwenyao et al.Multiscale engineered artificial compact bone via bidirectional freeze-driven lamellated organization of mineralized collagen microfibrilsBioactive Materials2024
Iwasaki, Yasuhiko et al.Suppression of bone resorption in ovariectomized mice using estrogen-immobilized polyphosphodiestersMaterialia2024
Yang, Chun Yi et al.Multi-needle blow-spinning technique for fabricating collagen nanofibrous nerve guidance conduit with scalable productivity and high performanceMaterials Today Bio2024
Chen, Mengxuan et al.The redox-sensitive GSK3β is a key regulator of glomerular podocyte injury in type 2 diabetic kidney diseaseRedox Biology2024
Wu, Xueping et al.Silibinin attenuates TGF-β2-induced fibrogenic changes in human trabecular meshwork cells by targeting JAK2/STAT3 and PI3K/AKT signaling pathwaysExperimental Eye Research2024
Seki, Hiromu et al.Involvement of RAGE in radiation-induced acquisition of malignant phenotypes in human glioblastoma cellsBiochimica et Biophysica Acta (BBA) - General Subjects2024
Bhattacharjee, Abhishek et al.Titania (TiO 2 ) nanotube surfaces doped with zinc and strontium for improved cell compatibilityNanoscale2024
Chun-Yi Chang et al.Triple click chemistry for crosslinking, stiffening, and annealing of gelatin-based microgelsRSC Applied Polymers2024
Xiang, Jingzhou et al.Exo70 Promotes the Invasion of Pancreatic Cancer Cells via the Regulation of ExosomesCancers2024
Ishikawa, Soichiro et al.EP4-induced mitochondrial localization and cell migration mediated by CALML6 in human oral squamous cell carcinomaCommunications Biology 2024
Liu, Huan et al.MSC-derived exosomes protect auditory hair cells from neomycin-induced damage via autophagy regulationBiological Research2024
Shimizu, Shota et al.An Autotaxin-Induced Ocular Hypertension Mouse Model Reflecting Physiological Aqueous BiomarkerInvestigative Ophthalmology & Visual Science2024
Harada, Shotaro et al.A mouse model of autoimmune inner ear disease without endolymphatic hydropsBiochimica et Biophysica Acta (BBA) - Molecular Basis of Disease2024
Tian, Chaoyong et al.Norep********* protects against cochlear outer hair cell damage and noise-induced hearing loss via α2A-adrenergic receptorBMC Neuroscience2024
Hua, Wei et al.CD36-mediated podocyte lipotoxicity promotes foot process effacementOpen Medicine (Poland)2024
Li, Jun et al.Helicobacter pylori promotes gastric fibroblast proliferation and migration by expulsing exosomal miR-124-3pMicrobes and Infection2024
Eddington, Casey et al.filoVision – using deep learning and tip markers to automate filopodia analysisJournal of Cell Science2024
Nie, Sheng et al.Effects of extract solution from magnesium alloys supplemented with different compositions of rare earth elements on in vitro epithelial and osteoblast progenitor cellsFrontiers in Bioengineering and Biotechnology2023
Hao, Xiaotian et al.3D printed structured porous hydrogel promotes osteogenic differentiation of BMSCsMaterials & Design2023
Nakashima, Mio et al.Pharmacologic Comparison of High-Dose Hesperetin and Quercetin on MDCK II Cell Viability, Tight Junction Integrity, and Cell ShapeAntioxidants2023
Li, Jia et al.Titanium surface interacting with blood clot enhanced migration and osteogenic differentiation of bone marrow mesenchymal stem cellsFrontiers in Bioengineering and Biotechnology2023
Wang, Xiaofang et al.A novel decellularized matrix of Wnt signaling-activated osteocytes accelerates the repair of critical-sized parietal bone defects with osteoclastogenesis, angiogenesis, and neurogenesisBioactive Materials2023
Ma, Yufei et al.Extended preconditioning on soft matrices directs human mesenchymal stem cell fate via YAP transcriptional activity and chromatin organizationAPL Bioengineering2023
Zhao, Juan et al.IRAK4 inhibition: an effective strategy for immunomodulating peri-implant osseointegration via reciprocally-shifted polarization in the monocyte-macrophage lineage cellsBMC Oral Health2023
Kim, JA, et al.Britanin inhibits titanium wear particle‑induced osteolysis and osteoclastogenesisMolecular Medicine Reports2023
Yang, Xiaosong et al.Ulinastatin ameliorates podocyte ferroptosis via regulating miR-144-3p/SLC7A11 axis in acute kidney injuryIn Vitro Cellular and Developmental Biology2023
Comelles, Jordi et al.Soft topographical patterns trigger a stiffness-dependent cellular response to contact guidanceMaterials Today Bio2023
Tan, Jun Kai et al.Evaluation of trabecular meshwork-specific promoters in vitro and in vivo using scAAV2 vectors expressing C3 transferaseInternational Journal of Ophthalmology2023
Wang, Chunqing et al.PKCζ phosphorylates VASP to mediate chemotaxis in breast cancer cellsExperimental Cell Research2023
Chang, Yongyun et al.Aspirin prevents estrogen deficiency-induced bone loss by inhibiting osteoclastogenesis and promoting osteogenesisJournal of Orthopaedic Surgery and Research2023
Nanmo, Ayaka et al.Bioprinting of hair follicle germs for hair regenerative medicineActa Biomaterialia2023
Krey, Jocelyn F. et al.Control of stereocilia length during development of hair bundlesPLOS Biology2023
Liu, Yang et al.Sulforaphane inhibits TGF-β-induced fibrogenesis and inflammation in human Tenon’s fibroblastsMolecular Vision2023
Yoshida, Koki et al.Macroscale Collagen-Actomyosin Hybrid Actuator Built from Bioderived MaterialsAdvanced Functional Materials2023
Ding, Xiaochu et al.A Next-Generation 3D Tissue-Engineered Model of the Human Brain Microvasculature to Study the Blood-Brain BarrierBioengineering2023
Whitehead, Clarissa A. et al.Invadopodia associated Thrombospondin-1 contributes to a post-therapy pro-invasive response in glioblastoma cellsExperimental Cell Research2023
Macedo, Maria Helena et al.The shape of our gut: Dissecting its impact on drug absorption in a 3D bioprinted intestinal modelBiomaterials Advances2023
Talukdar, Sattya N. et al.Respiratory Syncytial Virus Infection Does Not Induce Epithelial-Mesenchymal TransitionJournal of Virology2023
Zhu, Guang‐Jie et al.Cingulin regulates hair cell cuticular plate morphology and is required for hearing in human and mouseEMBO Molecular Medicine2023
Shimizu, Mikito et al.RGMa collapses the neuronal actin barrier against disease-implicated protein and exacerbates ALSScience Advances2023
Cai, Guanhui et al.Piezo1-mediated M2 macrophage mechanotransduction enhances bone formation through secretion and activation of transforming growth factor-β1Cell Proliferation2023
Wu, Yuanyuan et al.HBSP improves kidney ischemia-reperfusion injury and promotes repair in properdin deficient mice via enhancing phagocytosis of tubular epithelial cellsFrontiers in Immunology2023
Moon, So Youn et al.Cell contractility drives mechanical memory of oral squamous cell carcinomaMolecular Biology of the Cell2023
Whitehead, Clarissa A. et al.Small extracellular vesicles promote invadopodia activity in glioblastoma cells in a therapy-dependent mannerCellular Oncology2023
Foda, Bardees M. et al.Role of Rho/MRTF in Aggressive Vemurafenib-Resistant Murine Melanomas and Immune Checkpoint UpregulationInternational Journal of Molecular Sciences2023
Tian, Chaoyong et al.Expression and localization of α2A-adrenergic receptor in the rat post-natal developing cochleaEuropean Journal of Histochemistry : EJH2023
Zhu, Xinglong et al.Tissue-specific micropattern array chips fabricated via decellularized ECM for 3D cell cultureMethodsX2023
Talukdar, Sattya N. et al.RSV-induced expanded ciliated cells contribute to bronchial wall thickeningVirus Research2023
Müller, Joachim et al.Targeted and non-targeted proteomics to characterize the parasite proteins of Echinococcus multilocularis metacestodesFrontiers in Cellular and Infection Microbiology2023
Jerome, Kailey et al.Simultaneous detection and quantification of spike mRNA and protein in SARS-CoV-2 infected airway epithelium.MethodsX2023
Gan, Ziqi et al.Stiffness-tuned and ROS-sensitive hydrogel incorporating complement C5a receptor antagonist modulates antibacterial activity of macrophages for periodontitis treatmentBioactive Materials2023
Andrews, S. L. et al.SVEP1 influences monocyte to macrophage differentiation via integrin α4β1/α9β1 and Rho/Rac signallingBiochimica et Biophysica Acta (BBA) - Molecular Cell Research2023
Wheatley, Brittany A. et al.Nanotopography modulates cytoskeletal organization and dynamics during T cell activationMolecular Biology of the Cell2022
Zheng, Jingchuan et al.Biphasic mineralized collagen-based composite scaffold for cranial bone regeneration in developing sheepRegenerative Biomaterials2022
Bai, Peng et al.A Novel Method to Improve the Physical Property and Biocompatibility of Decellularized Heart Valve Scaffold with Sericin and PolydopamineJournal of Bionic Engineering2022
Ito, Reiji et al.SM22α cell-specific HIF stabilization mitigates hyperoxia-induced neonatal lung injuryAmerican journal of physiology. Lung cellular and molecular physiology2022
Xiang, Haibo et al.Cryogenic 3D printing of bifunctional silicate nanoclay incorporated scaffolds for promoted angiogenesis and bone regenerationMaterials & Design2022
Flores-Muñoz, Carolina et al.The Long-Term Pannexin 1 Ablation Produces Structural and Functional Modifications in Hippocampal NeuronsCells2022
Xu, Weiyi et al.Paxillin promotes breast tumor collective cell invasion through maintenance of adherens junction integrityhttps://doi.org/10.1091/mbc.E21-09-04322022
Lin, Lin et al.Phase separation-mediated condensation of Whirlin-Myo15-Eps8 stereocilia tip complexCell Reports2021
Jozic, Ivan et al.Glucocorticoid-mediated induction of caveolin-1 disrupts cytoskeletal organization, inhibits cell migration and re-epithelialization of non-healing woundsCommunications Biology2021
Hu, Ke et al.Dual anisotropicity comprising 3D printed structures and magnetic nanoparticle assemblies: towards the promotion of mesenchymal stem cell osteogenic differentiationNPG Asia Materials 2021
Stoecklein, Katy S. et al.Improved cryopreservation of in vitro produced bovine embryos using FGF2, LIF, and IGF1PLOS ONE2021
Fukusumi, Yoshiyasu et al.Nephrin–Ephrin-B1–Na+/H+ Exchanger Regulatory Factor 2–Ezrin–Actin Axis Is Critical in Podocyte InjuryThe American Journal of Pathology2021
Cenariu, Diana et al.Hsa-miR-125b Therapeutic Role in Colon Cancer Is Dependent on the Mutation Status of the TP53 GenePharmaceutics2021
Marchetti, Sara et al.Combustion-derived particles from biomass sources differently promote epithelial-to-mesenchymal transition on A549 cellsArchives of toxicology2021
Bakshi, Mariam et al.Ostertagia ostertagi mediates early host immune responses via macrophage and toll-like receptor pathwaysInfection and Immunity2021
Ihn, Hye Jung et al.PF-3845, a Fatty Acid Amide Hydrolase Inhibitor, Directly Suppresses Osteoclastogenesis through ERK and NF-κB Pathways In Vitro and Alveolar Bone Loss In VivoInternational Journal of Molecular Sciences2021
Nishimura, Yushi et al.Wide-field fluorescent nanodiamond spin measurements toward real-time large-area intracellular thermometryScientific Reports 2021
Lee, Geonhui et al.Cell-ECM contact-guided intracellular polarization is mediated via lamin A/C dependent nucleus-cytoskeletal connectionBiomaterials2021
Nishiguchi, Koji M. et al.A hypomorphic variant in EYS detected by genome-wide association study contributes toward retinitis pigmentosaCommunications Biology 2021
Huang, Yan Bing et al.Inhibition of TGF-β2-induced migration and epithelial-mesenchymal transition in ARPE-19 by sulforaphaneInternational Journal of Ophthalmology2021
Zhou, Xuefeng et al.Ca ions chelation, collagen I incorporation and 3D bionic PLGA/PCL electrospun architecture to enhance osteogenic differentiationMaterials & Design2021
Zerboni, Alessandra et al.Cellular mechanisms involved in the combined toxic effects of diesel exhaust and metal oxide nanoparticlesNanomaterials2021
Zhao, Fengyuan et al.Comparison of three different acidic solutions in tendon decellularized extracellular matrix bio-ink fabrication for 3D cell printingActa Biomaterialia2021
Logsdon, Deirdre M. et al.Evaluation of the TMRW vapor phase cryostorage platform using reproductive specimens and in vitro extended human embryo cultureF&S Science2021
Xing, Qianqian et al.Scavenger receptor MARCO contributes to macrophage phagocytosis and clearance of tumor cellsExperimental Cell Research2021
Danielsson, Brooke E. et al.Lamin microaggregates lead to altered mechanotransmission in progerin-expressing cellsNucleus2020
Lu, Fanqing et al.SILAC-based proteomic profiling of the suppression of TGF-β1-induced lung fibroblast-to-myofibroblast differentiation by trehaloseToxicology and applied pharmacology2020
Huang, Yuxing et al.Arp2/3-branched actin maintains an active pool of GTP-RhoA and controls RhoA abundanceCells2019
Cavalcanti, José Henrique de Lima et al.In vitro assessment of the functional dynamics of titanium with surface coating of hydroxyapatite nanoparticlesMaterials2019
Pena-Philippides, Juan Carlos et al.Inhibition of microRNA-155 supports endothelial tight junction integrity following oxygen-glucose deprivationJournal of the American Heart Association2018
Liang, Fengyi et al.Juxtanodin in retinal pigment epithelial cells: Expression and biological activities in regulating cell morphology and actin cytoskeleton organizationJournal of Comparative Neurology2018
Cetera, Maureen et al.Counter-rotational cell flows drive morphological and cell fate asymmetries in mammalian hair folliclesNature Cell Biology2018
Zhang, Ying et al.Effects of testosterone on the expression levels of AMH, VEGF and HIF-1α in mouse granulosa cellsExperimental and Therapeutic Medicine2016
Nader, Guilherme P.F. et al.FAK, talin and PIPKI 3 regulate endocytosed integrin activation to polarize focal adhesion assemblyNature Cell Biology2016
Larabee, Shannon M. et al.MiRNA-17 members that target Bmpr2 influence signaling mechanisms important for embryonic stem cell differentiation in vitro and gastrulation in embryosStem Cells and Development2015
Kumeta, Masahiro et al.Caprice/MISP is a novel F-actin bundling protein critical for actin-based cytoskeletal reorganizationsGenes to Cells2014
Beriault, Daniel R. et al.The mechanical behavior of mutant K14-R125P keratin bundles and networks in NEB-1 keratinocytesPLoS ONE2012
Gao, Chengzhuo et al.Histone deacetylase 7 (HDAC7) regulates myocyte migration and differentiationBiochimica et Biophysica Acta - Molecular Cell Research2010

Question 1:  Can I use fluorescently-labeled phalloidin to stain F-actin in living cells?

Answer 1:  Unfortunately, no, phalloidin cannot be used to stain F-actin in living cells.  Phalloidins are used to stain F-actin in fixed cells.  Fluorescent phalloidins only bind to the native quaternary structure of F-actin which provides a low background.  The correct fixation condition for phalloidin binding is 3.7% (v/v) paraformaldehyde in PBS for 10 min because it retains the quaternary protein structure which is necessary for high affinity binding of phalloidin.  Methanol fixation destroys the native conformation and hence is not suitable for F-actin staining with phalloidin.  To monitor actin dynamics in living cells, micro-injection of rhodamine-labeled actin (Cat. # APHR or AR05) is recommended.  Please see those datasheets for more information.

 

Question 2:  Which of the fluorescently-labeled phalloidin is the most stable/brightest?

Answer 2:  The brightest and most stable of the Acti-stains is Acti-stain 488 (green fluorescence; Cat. # PHDG1).  Please see the table below for additional information on all of our Acti-stains. 

Conjugate

Cat. #

Wavelengths (Ex/EM)

Brightness (AFU)

Stability to photobleaching* (1/2 life in sec)

Background (% of total AFU at 100 nM)

Acti-stain™ 488

PHDG1

485/535

832

57

5

Acti-stain™ 535

PHDR1

535/585

430

27

12

Acti-stain™ 555

PHDH1

535/585

551

46

16

Acti-stain™ 670

PHDN1

640/680

332

8

18

* = measured in the absence of antifade.

 

If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com.