Actin polymerization buffer: 10x stock
Product Uses Include
- Polymerization of G-actin into F-actin
Material
This is a 10 x solution that contains 500 mM KCl, 20 mM MgCl2, 50 mM guanidine carbonate pH 7.5, and 10 mM ATP in 100 mM Tris, pH 7.5. It is used to add to G-actin solutions in A-buffer (General actin buffer (Cat. # BSA01), 0.2 mM ATP (Cat. # BSA04) and 0.5 mM DTT) to induce polymerization into F-actin.
For product Datasheets and MSDSs please click on the PDF links below. For additional information, click on the FAQs tab above or contact our Technical Support department at tservice@cytoskeleton.com
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Question 1: Once resuspended, can left-over actin polymerization buffer be stored at 4°C to maintain activity?
Answer 1: Upon reconstitution (2.0 ml of 100 mM Tris-HCl pH 7.5), the actin polymerization buffer (Cat. # BSA02) will be at 10X strength. Set aside the volume needed for that day’s experiments (This buffer is stable for 30 min on ice) and aliquot the remainder into 100 μl volumes, snap frozen in liquid nitrogen, and stored at -70°C. These stocks are stable for 6 months at -70°C.
Question 2: After resuspension, does the actin polymerization buffer need to be used in a certain amount of time before losing activity?
Answer 2: The actin polymerization buffer (Cat. # BSA02) will be stable for 30 min after thawing the previously frozen aliquots (or using freshly prepared). Keep on ice until ready to use. We do not recommend re-freezing aliquots.
If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com
