Actin polymerization buffer: 10x stock

Actin polymerization buffer: 10x stock

Product Uses Include

  • Polymerization of G-actin into F-actin


This is a 10 x solution that contains 500 mM KCl, 20 mM MgCl2, 50 mM guanidine carbonate pH 7.5, and 10 mM ATP in 100 mM Tris, pH 7.5. It is used to add to G-actin solutions in A-buffer (General actin buffer (Cat. # BSA01), 0.2 mM ATP (Cat. # BSA04) and 0.5 mM DTT) to induce polymerization into F-actin.

For product Datasheets and MSDSs please click on the PDF links below.   For additional information, click on the FAQs tab above or contact our Technical Support department at

AuthorTitleJournalYearArticle Link
Delaunay, Marion et al.AKAP2-anchored extracellular signal-regulated kinase 1 (ERK1) regulates cardiac myofibroblast migrationBiochimica et Biophysica Acta (BBA) - Molecular Cell Research2024ISSN 0167--4889
Arbore, C. et al.α-catenin switches between a slip and an asymmetric catch bond with F-actin to cooperatively regulate cell junction fluidityNature Communications2022ISSN 2041-1723
Giampazolias, Evangelos et al.Secreted gelsolin inhibits DNGR-1-dependent cross-presentation and cancer immunityCell2021ISSN 1097-4172
Park, Jin Suk et al.Mechanical regulation of glycolysis via cytoskeleton architectureNature2020ISSN 1476-4687
Ergin, Volkan et al.Putative Coiled-Coil Domain-Dependent Autoinhibition and Alternative Splicing Determine SHTN1’s Actin-Binding ActivityJournal of Molecular Biology2020ISSN 1089-8638
Gardini, Lucia et al.A protocol for single molecule imaging and tracking of processive myosin motorsMethodsX2019ISSN 2215-0161
Limatola, Nunzia et al.Nicotine Induces Polyspermy in Sea Urchin Eggs through a Non-Cholinergic Pathway Modulating Actin DynamicsCells2019ISSN 2073-4409
Piroli, Gerardo G. et al.Identification of novel protein targets of DMF modification in neurons and astrocytes reveals actions independent of Nrf2 stabilizationMolecular and Cellular Proteomics2019ISSN 1535-9484
Laitila, Jenni et al.A nebulin super-repeat panel reveals stronger actin binding toward the ends of the super-repeat regionMuscle and Nerve2019ISSN 1097-4598
Patel, Vaibhav B. et al.PI3Kα-regulated gelsolin activity is a critical determinant of cardiac cytoskeletal remodeling and heart diseaseNature Communications2018ISSN 2041-1723
Gardini, Lucia et al.Dissecting myosin-5B mechanosensitivity and calcium regulation at the single molecule levelNature Communications2018ISSN 2041-1723
Gardini, L. et al.High-speed optical tweezers for the study of single molecular motorsMethods in Molecular Biology2018ISSN 1064-3745
Goryunov, D et al.Microtubule-actin cross-linking factor 1: domains, interaction partners, and tissue-specific functionsMethods in Enzymology2016Article Link
Wang, Y et al.Fluorescence imaging with one-nanometer accuracy (FIONA)JoVE (Journal of …2014Article Link
Garlick, Kristopher M. et al.Direct interaction between anthrax toxin receptor 1 and the actin cytoskeletonBiochemistry2009ISSN 0006-2960
Allingham, John S. et al.A Structural Basis for Regulation of Actin Polymerization by PectenotoxinsJournal of Molecular Biology2007ISSN 0022-2836
Allingham, John S. et al.Structures of microfilament destabilizing toxins bound to actin provide insight into toxin design and activityProceedings of the National Academy of Sciences of the United States of America2005ISSN 0027-8424
Goryunov, Dmitry et al.Studying cytolinker proteinsMethods in Cell Biology2004ISSN 0091-679X


Question 1: Once resuspended, can left-over actin polymerization buffer be stored at 4°C to maintain activity?

Answer 1: Upon reconstitution (2.0 ml of 100 mM Tris-HCl pH 7.5), the actin polymerization buffer (Cat. # BSA02) will be at 10X strength.  Set aside the volume needed for that day’s experiments (This buffer is stable for 30 min on ice) and aliquot the remainder into 100 μl volumes, snap frozen in liquid nitrogen, and stored at -70°C. These stocks are stable for 6 months at -70°C.


Question 2: After resuspension, does the actin polymerization buffer need to be used in a certain amount of time before losing activity?

Answer 2: The actin polymerization buffer (Cat. # BSA02) will be stable for 30 min after thawing the previously frozen aliquots (or using freshly prepared). Keep on ice until ready to use.  We do not recommend re-freezing aliquots.



If you have any questions concerning this product, please contact our Technical Service department at