• Buffer of choice for the polymerization of actin proteins
• Used to create actin filaments
• Compatible with the polymerization of labeled actin proteins
• Compatible with actin proteins from a wide range of species

Supplied as a lyophilized powder. When resuspended in 2 ml of 100 mM Tris HCl, pH 7.5, it provides a 10x working strength buffer consisting of 100 mM Tris HCl, pH 7.5, 500 mM potassium chloride, 20 mM magnesium chloride, 10 mM adenosine 5’ triphosphate disodium salt (ATP) (50 mM guanidine carbonate is present to stabilize ATP).

Storage and handling

• After resuspension, aliquot into experiment-sized volumes and store at –80 °C to prevent ATP hydrolysis
• Once thawed, keep the buffer on ice and use within 1–2 hours
• Discard any unused thawed buffer

All reagents used in this buffer are ≥ American Chemical Society (ACS) grade and ≥99% pure

The biological activity of BSA02 is confirmed by its ability to polymerize rabbit skeletal muscle actin AKL99 to ≥90% actin filaments.