• Rabbit skeletal muscle actin is a good choice for in vitro assays requiring actin filaments as a general substrate. Cyto offers specialized actins that may be more suited to non-skeletal muscle applications
• Measure in vitro skeletal muscle actin polymerization kinetics via fluorescence increase
• Actin-binding protein (ABP) effect on actin dynamics
• Drug effects on actin-ABP interactions

Rabbit skeletal muscle actin AKL99 is labeled with pyrene (N-1-pyrene iodoacetamide) at the reactive cysteine 374 residue:

• Ab: 360 nm, Em: 405 nm
• Labeling stoichiometry: ~0.4-0.6 pyrenes per actin monomer.
• Supplied as a lyophilized powder

Protein purity is assessed by scanning densitometry of Coomassie Blue stained protein on a12% polyacrylamide gel. Purity was determined to be >99% pure

The biological activity of pyrene-labeled actin has been determined by its ability to polymerize into filaments in vitro efficiently. Pyrene actin exhibits a 7–12-fold increase in fluorescence (Ex: 360 nm, Em: 405 nm) upon polymerization, equivalent to >80% polymerization.