Protocol video highlighting key steps and tools used in this comprehensive enrichment system.

The use of peer-reviewed, video articles to report technical methodology are an expanding resource that has great benefits compared to text formats, such as providing intricate details that are not effectively captured in written manuscripts. Recently, Horita et al. employed this format to describe a methodology for investigating post-translational modification (PTM) crosstalk.

  • Step-by-step instructions to detect acetylation, ubiquitination, SUMOylation 2/3, and tyrosine phosphorylation in a single lysate.
  • Highlights critical components/steps such as a lysate filter system that effectively removes, rather than shears, contaminating genomic DNA. 
  • Developed for use with the Signal-Seeker™ PTM detection kits that are effective tools for examining dynamic and endogenous levels of several different PTMs. 

As investigators move forward with PTM research and investigate a specific PTM’s physiologic role, it will be paramount to have effective tools and methodology, like those described in this study, to measure endogenous levels of a PTM for a target protein.

Link to citation:

Products used in this citation:

Signal-Seeker™ Phosphotyrosine Detection Kit (30 assay) (Cat. # BK160)

Signal-Seeker™ Ubiquitination Detection Kit (30 assay) (Cat. # BK161)

Signal-Seeker™ SUMOylation 2/3 Detection Kit (30 assay) (Cat. # BK162)

Signal-Seeker™ Acetyl-Lysine Detection Kit (30 assay) (Cat. # BK163)

BlastR Rapid Lysate Prep Kit (Cat. # BLR01)

BlastR Rapid Filtration Kit (Cat. # BLR02)

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alex castellanos