• Use FLIM microscopy to measure membrane tension changes in the plasma membrane of living cells

Flipper-TR® is a fluorogenic probe that specifically targets the plasma membrane of cells and reports membrane tension fluctuations through its fluorescence lifetime changes. It senses changes of the organization of lipid bilayer membranes through changes of the twist angle and polarization between the two twisted dithienothiophenes of the mechanophore. Flipper-TR spontaneously inserts into the plasma membrane of cells and is only fluorescent when inserted in a lipid membrane.

Key features

• Ab: 485 or 488 nm pulsed laser, Em: 600±50 nm
• Works on a wide range of organisms including bacteria, yeast, mammals and plants
• Fluorogenic character: enhanced fluorescence when inserted into plasma membrane
• Requires FLIM super resolution microscopy

The biological activity of CY-SC020 is assessed by the ability of the probe to efficiently detect plasma membrane tension changes in live cell culture.