• End point measurement of microtubule-activated kinesin ATPase activity
• High-throughput discovery of kinesin inhibitors

Kit contents (960 assays)

• CytoPhos reagent
• Phosphate standard
• Kinesin motor protein
• Pre-formed microtubules
• Kinesin reaction buffer
• Paclitaxel
• DMSO

Equipment & materials required

• Spectrophotometer, end-point setting, Absorbance 650 nm

The microtubule-activated kinesin ATPase assay uses a malachite green/molybdate-based colorimetric reaction, in which the release of inorganic phosphate (Pi) during ATP hydrolysis causes a visible color shift from yellow to green. This method is a rapid and cost-effective method for kinesin inhibitor drug discovery.

Key characteristics

• Quantitative assay
• Detection method: end-point absorbance 650 nm
• Assay volume: 100 µl
• Sensitivity: 0.05 – 2.5 nmoles Pi (100 µl volume)
• Timeframe: Approximately 2 hours