SiR700-Lysosome Kit

Live human fibroblast cells stained with 1 µM SiR700-lysosome (red) and Hoechst (blue) for 1 h at 37°C and imaged by widefield microscopy.

SiR700-lysosome is based on the pepstatin A (a cathepsin D binding peptide) which has been labeled with the proprietary far-red fluorophore called silicon rhodamine 700 (SiR700). SiR700-lysosome is fluorogenic, cell permeable and highly specific for lysosomes. It stains early and late endocytic vesicles and lysosomes in live cells without the need for genetic manipulation or overexpression. Its emission in the far red minimizes phototoxicity and sample autofluorescence. SiR700-lysosome is compatible in dual fluorescence studies with other SIR dye conjugates, GFP and/or mCherry fluorescent proteins. It can be imaged with standard Cy5 filter sets, or high resolution filters for dual staining. SiR700-lysosome can be used for widefield, confocal, SIM or STED imaging in living cells and tissue. Probe quantity allows 35 – 140 staining experiments*. 

Optical properties

λ_abs    689 nm
λ_ex      716 nm

ε_max   1.0·10⁵ mol^-1·cm^-1

MW     1262.7 g/mol

MF      C₆₉H₁₀₃N₉O₁₁Si

*Based on the following conditions: 0.5 – 1 ml staining solution / staining experiments with 0.5 – 1 µM probe concentration. The number of staining experiments can be further increased by reducing volume or probe concentration.

Cytoskeleton, Inc. is the exclusive provider of Spirochrome, Ltd. products in North America.

For product Datasheets and MSDSs please click on the PDF links below.

Product Description

MSDS

Datasheet

Cat #

SiR700-Lysosome Kit CY-SC016

Originating Publication 

Lukinavicius G. et al. 2016. Fluorogenic probes for multi-color imaging in living cells.  J. Am. Chem. Soc. Doi: 10.1021/jacs.6b04782.

Lukinavicius, G. et al. 2015. SiR-Hoechst is a far-red DNA stain for live-cell nanoscopy. Nat. Commun. 6:8497 doi:10.1038/ncomms9497 (2015).

For comparison information with other nuclear stains click here.